1887

Abstract

SUMMARY: Cell walls prepared from vegetative yeast cells and from hormone-induced conjugation tubes of the basidiomycete had similar compositions. Evidence was found for 1,3-α-glucan (yeast 38%, tube 25%), 1,3--1,6--glucan (yeast 33%, tube 48%) and chitin (both < 3%) in the walls. The walls also contained xylose (5 to 7%), mannose (6%), glucuronic acid (approx. 2%), and traces of galactose. Protein amounted to less than 2% of the wall weight. The cell capsule was very insoluble and could not be removed from the cell wall. The conjugation hormone did not appear to exert its effect on cell shape by causing gross changes in wall composition.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-96-1-35
1976-09-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/96/1/mic-96-1-35.html?itemId=/content/journal/micro/10.1099/00221287-96-1-35&mimeType=html&fmt=ahah

References

  1. Angyal S. J., Bender V. J., Ralph B. J. 1974; Structure of polysaccharides from thePolyporus tumulosus cell wall. Biochimica et biophysica acta 362:175–187
    [Google Scholar]
  2. Ankel H., Ankel E., Schxttzbach J. S., Garancis J. C. 1970; Mannosyl transfer inCryptococcus laurentii. Journal of Biological Chemistry 245:3945–3955
    [Google Scholar]
  3. Bacon J.S.D., Jones D., Farmer V. C., Webley D. M. 1968; The occurrence of a α-(I→3)-glucan inCryptococcus, Schizosaccharomyces andPolyporus species, and its hydrolysis by aStreptomyces culture filtrate lysing cell walls ofCryptococcus. Biochimica et biophysica acta 158:313–315
    [Google Scholar]
  4. Bandoni R. J. 1965; Secondary control of conjugation inTremella mesenterica. Canadian Journal of Botany 43:627–630
    [Google Scholar]
  5. Bartnicki-Garcia S., Lindberg B. 1972; Partial characterization of mucoran: the glucuronomannan component. Carbohydrate Research 23:75–85
    [Google Scholar]
  6. Bartnicki-Garcia S., Lippman E. 1969; Fungal morphogenesis. Cell wall construction inMucor rouxii. Science; New York: 165:302–304
    [Google Scholar]
  7. Bartnicki-Garcia S., Nickerson W. J. 1962; Isolation, composition and structure of cell walls of filamentous and yeast-like forms ofMucor rouxii. Biochimica et biophysica acta 58:102–119
    [Google Scholar]
  8. Bitter T., Muir H. M. 1962; A modified uronic acid carbazole reaction. Analytical Biochemistry 4:330–334
    [Google Scholar]
  9. Blake J. D., Richards G. N. 1970; A critical re-examination of problems inherent in compositional analysis of hemicelluloses by gas-liquid chromatography. Carbohydrate Research 14:375–387
    [Google Scholar]
  10. Bouveng H. O., Fraser R. N., Lindberg B. 1967; Polysaccharides elaborated byArmillaria mellea(Tricholomataceae). II. Water-soluble mycelium polysaccharides. Carbohydrate Research 4:20–31
    [Google Scholar]
  11. Cameron D. S., Taylor I.E.P. 1976; Quantitative microanalysis of cell walls ofSaprolegnia diclina Humphrey andTremella mesenterica Fries. Biochimica et biophysica acta (in the Press).
    [Google Scholar]
  12. Crook E. M., Johnston I. R. 1962; The qualitative analysis of the cell walls of selected species of fungi. Biochemical Journal 86:325–331
    [Google Scholar]
  13. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  14. Fraser C. G., Jennings H. J. 1971; A glucan fromTremella mesenterica NRRL-Y6158. Canadian Journal of Chemistry 49:1804–1807
    [Google Scholar]
  15. Fraser C. G., Jennings H. J., Moyna P. 1973; Structural analysis of an acidic polysaccharide fromTremella mesenterica NRRL-Y6158. Canadian Journal of Biochemistry 51:219–224
    [Google Scholar]
  16. Gatt R., Berman E. R. 1966; A rapid procedure for the estimation of amino sugars on a micro scale. Analytical Biochemistry 15:167–171
    [Google Scholar]
  17. Houwink A. L., Kreger D. R. 1953; Observations on the cell wall of yeasts. An electron microscope and X-ray diffraction study. Antonie van Leeuwenhoek 19:1–24
    [Google Scholar]
  18. Huotari F. I., Nelson T. E., Smith F., Kirkwood S. 1968; Purification of anexo-β-D-{1→3)- glucanase fromBasidiomycete species QM8O6. Journal of Biological Chemistry 243:952–956
    [Google Scholar]
  19. Jones J.K.N., Stoodley R. J. 1965; Fractionation using copper complexes. in Methods in Carbohydrate Chemistry, 5:36 Whistler. R. L. Edited by New York:: Academic Press.;
    [Google Scholar]
  20. Kanetsuna F., Carbonell L. M., Azuma I., Yamamura Y. 1972; Biochemical studies on the thermal dimorphism ofParacoccidioides brasiliensis. Journal of Bacteriology 110:208–218
    [Google Scholar]
  21. Kanetsuna F., Carbonell L. M., Gil F., Azuma I. 1974; Chemical and ultrastructural studies on the cell walls of the yeast-like and mycelial forms ofHistoplasma capsulatum. Mycopathologia et mycologia applicata 54:1–13
    [Google Scholar]
  22. Lippman E., Erwin D. C., Bartnicki-Garcia S. 1974; Isolation and chemical composition of oospore- oogonium walls ofPhytophthora megasperma var.sojae. Journal of General Microbiology 80:131–141
    [Google Scholar]
  23. Michell A. J., Scurfield G. 1970; An assessment of infrared spectra as indicators of fungal cell wall composition. Australian Journal of Biological Sciences 23:345–360
    [Google Scholar]
  24. Moulki H., Bonaly R. 1974; Etude des parois de levures du genreRhodotorula. IV. Variation de la teneur en chitine. Biochimica et biophysica acta 338:120–134
    [Google Scholar]
  25. O'BRIEN R. W., Ralph B. J. 1966; The cell wall composition and taxonomy of some Basidiomycetes and Ascomycetes. Annals of Botany, N.S. 30:831–843
    [Google Scholar]
  26. Reid I. D. 1974; Properties of conjugation hormones (erogens) from the basidiomyceteTremella mesenterica. Canadian Journal of Botany 52:521–524
    [Google Scholar]
  27. Reid I. D. 1975; Growth ofTremella mesenterica haplonts with various nitrogen sources. Canadian Journal of Botany 53:73–77
    [Google Scholar]
  28. Slodki M. E., Wickerham L. J., Bandoni R. J. 1966; Extracellular heteropolysaccharides fromCryptococcus andTremella: a possible taxonomic relationship. Canadian Journal of Microbiology 12:489–494
    [Google Scholar]
  29. Spackman D. H., Stein W. H., Moore S. 1958; Automatic recording apparatus for use in the chromatography of amino acids. Analytical Chemistry 30:1190–1206
    [Google Scholar]
  30. Tracey M. V. 1955; Chitin. In Modern Methods of Plant Analysis, 2: pp. 269–280 Paech K., Tracey. M. V. Edited by Berlin:: Springer-Verlag.;
    [Google Scholar]
  31. Wang M. C., Bartnicki-Garcia S. 1970; Structure and composition of walls of the yeast form ofVerticillium albo-atrum. Journal of General Microbiology 64:41–54
    [Google Scholar]
  32. Wessels J.G.H., De Vries O.M.H. 1973; Wall structure, wall degradation, protoplast liberation and wall regeneration inSchizophyllum commune. . In Yeast, Mould, and Plant Protoplasts, pp. 295–306 Villanueva J. R., Garcia-Acha I., Gascon S., Uruburu. F. Edited by Londen New York:: Academic Press.;
    [Google Scholar]
  33. Wessels J.G.H., Kreger D. R., Marchant R., Regensburg B. A., De Vries O.M.H. 1972; Chemical and morphological characterization of the hyphal wall surface of the basidiomyceteSchizophyllum commune. Biochimica et biophysica acta 273:346–358
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-96-1-35
Loading
/content/journal/micro/10.1099/00221287-96-1-35
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error