1887

Abstract

SUMMARY: The differentiation of filaments of a continuous culture of after removal of fixed nitrogen has been examined. Rapid development of proheterocysts (4·5 h) and mature heterocysts (14 h) in an ordered sequence was observed; the development of the latter was concomitant with the onset of nitrogenase activity. Commitment times of 2·3 h (proheterocyst) and 5·0 h (heterocyst) were measured. Evidence is presented that shows that heterocyst development, rather than any product of heterocyst function in nitrogen fixation, is responsible for the imposition of the pattern of heterocysts in a filament of vegative cells. Both phycocyanin content and carbon dioxide fixation declined markedly throughout the filament during the early stages of heterocyst development, indicating that all the cells of the filament are involved in the initial stages of the differentiation process. Heterocysts do not evolve oxygen in the light, but do respire. Nitrogenase activity in isolated heterocysts and in intact filaments was stimulated by phosphoenolpyruvate, ATP and dichlorophenolindophenol-ascorbate.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-96-1-175
1976-09-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/96/1/mic-96-1-175.html?itemId=/content/journal/micro/10.1099/00221287-96-1-175&mimeType=html&fmt=ahah

References

  1. Allen M. B., Arnon D. I. 1955; Studies on nitrogen-fixing blue-green algae, 1. Growth and nitrogen fixation byAnabaena cylindrica. . Plant Physiology 30:366–372
    [Google Scholar]
  2. Allen M. B., Smith A. J. 1969; Nitrogen chlorosis in blue-green algae. Archiv für Mikrobiologie 69:114–120
    [Google Scholar]
  3. Bennett K. J., Silvester W. B., Brown J.M.A. 1975; The effect of pyruvate on nitrogenase activity in the blue-green algaAnabaena cylindrica. . Archives of Microbiology 105:61–66
    [Google Scholar]
  4. Bothe H., Falkenberg B. 1973; Demonstration and possible role of a ferredoxin-dependent pyruvate decarboxylation in the nitrogen fixing blue-green algaAnabaena cylindrica. . Plant Science Letters I:151–156
    [Google Scholar]
  5. Bradley S., Carr N. G. 1971; The absence of functional photosystem II in heterocysts ofAnabaena cylindrica. . Journal of General Microbiology 68:xiii–xiv
    [Google Scholar]
  6. Bulen W. A., Burns. R. C., Le Compte J. R. 1964; Nitrogen fixation : cell free system with extracts ofAzotobacter. . Biochemical and Biophysical Research Communications 17:265–271
    [Google Scholar]
  7. Carr N. G., Bradley S. 1973; Aspects of development in blue-green algae. Symposium of the Society for General Microbiology 23:161–188
    [Google Scholar]
  8. Codd G. A., Rowell P., Stewart W.D.P. 1974; Pyruvate and nitrogenase activity in cell-free extracts of the blue-green algaAnabaena cylindrica. . Biochemical and Biophysical Research Communications 61:424–431
    [Google Scholar]
  9. Donze M., Haveman J., Schiereck P. 1972; Absence of photosystem 2 in heterocysts of the blue-green algaAnabaena. . Biochimica et biophysica acta 256:157–161
    [Google Scholar]
  10. Drozd J. W., Tubb R. S., Postgate J. R. 1972; A chemostat study of the effect of fixed nitrogen sources on nitrogen fixation, membranes and free amino acids inAzotobacter chroococcum. . Journal of General Microbiology 73:221–232
    [Google Scholar]
  11. Fay P. 1973; Heterocysts. In The Biology of the Blue-Green Algae, pp. 238–259 Carr N. G., Whitton. B. A. Edited by Oxford :: Blackwells Scientific Publications.;
    [Google Scholar]
  12. Fay P., Walsby A. E. 1966; Metabolic activities of isolated heterocysts of the blue-green algaAnabaena cylindrica. . Nature; London: 209:94–95
    [Google Scholar]
  13. Fay P., Stewart W.D.P., Walsby A. E., Fogg G. E. 1968; Is the heterocyst the site of nitrogen fixation in blue-green algae ?. Nature; London: 220:810–812
    [Google Scholar]
  14. Fogg G. E. 1949; Growth and heterocyst production inAnabaena cylindrica Lemm. II. in relation to carbon and nitrogen metabolism. Annals of Botany, N.S. 13:241–259
    [Google Scholar]
  15. Itzhaki R. F., Gill D. M. 1964; A micro-biuret method for estimating proteins. Analytical Biochemistry 9:401–410
    [Google Scholar]
  16. Kulasooriya S. A., Fay F. 1974; On the reversibility of heterocyst differentiation. British Journal of Phycology 9:97–100
    [Google Scholar]
  17. Layne E. 1957; Spectrophotometric and turbidimetric methods for measuring protein. In Methods in Enzymology, I: pp. 447–454 Colowick S. P., Kaplan. N. O. Edited by New York:: Academic Press.;
    [Google Scholar]
  18. Leach C. K., Carr N. G. 1968; Reduced nicotinamide-adenine dinucleotide phosphate oxidase in the autotrophic blue-green alga, Anabaena variabilis. . Biochemical Journal 109:4P–5P
    [Google Scholar]
  19. Leach C. K., Carr N. G. 1970; Electron transport and oxidative phosphorylation in the blue-green algaAnabaena variabilis. . Journal of General Microbiology 64:55–70
    [Google Scholar]
  20. Leach C. K., Carr N. G. 1971; Pyruvate: ferredoxin oxidoreductase and its activation by ATP in the blue-green algaAnabaena variabilis. . Biochimica et biophysica acta 245:165–174
    [Google Scholar]
  21. Ohmori M., Hattori A. 1972; Effect of nitrate on nitrogen fixation by the blue-green algaAnabaena cylindrica. . Plant and Cell Physiology 13:589–599
    [Google Scholar]
  22. Stewart W.D.P., Lex M. 1970; Nitrogenase activity in the blue-green algaPlectonema boryanum strain 594. Archiv für Mikrobiologie 73:250–260
    [Google Scholar]
  23. Stewart W.D.P., Haystead A., Pearson H. W. 1969; Nitrogenase activity in heterocysts of blue-green algae. Nature; London: 224:226–228
    [Google Scholar]
  24. Tel-Or E., Stewart W.D.P. 1975; Manganese and photosynthetic oxygen evolution by algae. Nature; London: 258:715–716
    [Google Scholar]
  25. Thomas J. 1970; Absence of the pigments of photosystem II of photosynthesis in heterocysts of a blue-green alga. Nature; London: 228:181–183
    [Google Scholar]
  26. Tydgi V.V.S. 1975; The heterocysts of blue-green algae (Myxophyceae). Biological Reviews 50:247–284
    [Google Scholar]
  27. Vernon L. P., Kamen M. D. 1953; Studies in the metabolism of photosynthetic bacteria. XV. Photo-autoxidation of ferrocytochromec in extracts ofRhodospirillum rubrum. . Archives of Biochemistry and Biophysics 44:298–311
    [Google Scholar]
  28. Wilcox M., Mitchison G. J., Smith R. J. 1975; Spatial control of differentiation in the blue-green algaAnabaena. . In Microbiology-1975, pp. 453–463 Schlessinger. D. Edited by Washington:: American Society for Microbiology.;
    [Google Scholar]
  29. Wolk C. P. 1975; Differentiation and pattern formation in filamentous blue-green algae. In Spores VI, pp. 85–96 Gerhardt P., Sadoff H. L., Costilow. R. N. Edited by Washington:: American Society for Microbiology.;
    [Google Scholar]
  30. Wolk C. P., Wojciuch E. 1971; Photoreduction of acetylene by heterocysts. Planta 97:126–134
    [Google Scholar]
  31. Wyatt J. T., Silvey J.K.G. 1969; Nitrogen fixation byGloeocapsa. . Science; New York: 165:908–909
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-96-1-175
Loading
/content/journal/micro/10.1099/00221287-96-1-175
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error