1887

Abstract

SUMMARY: Using an enrichment procedure, mutant strains of were isolated that lacked the ability to utilize octopine as a nitrogen source. Of 55 such isolates, 44 were unable to utilize several amino acids; the remaining 11 strains were altered solely in their ability to utilize octopine, octopinic acid and lysopine. It is concluded that only the latter were plasmid mutations. Among them, there was a high, but no absolute, correlation with avirulence. All strains contained the T1 plasmid. All virulent strains showed active transport of octopine when they had previously been grown in medium containing octopine, whereas the avirulent strains failed to show such transport. All the virulent mutants induced tumours containing octopine. The results are discussed in relation to the hypothesis that the genes which code for the octopine synthesizing enzymes in the tumour are of bacterial origin.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-96-1-155
1976-09-01
2021-07-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/96/1/mic-96-1-155.html?itemId=/content/journal/micro/10.1099/00221287-96-1-155&mimeType=html&fmt=ahah

References

  1. Biemann K., Lioret C., Asselineau K., Lederer E., Polonski J. 1960; Sur la structure chimique de la lysopine, nouvelacide aminé isolé de tissu de crown-gall. Bulletin Société Chimique Biologique 42:979–991
    [Google Scholar]
  2. Bomhoff G. H. 1974 Studies on crown gall- a plant tumour. Investigations on protein composition and on the use of guanidine compounds as a marker for transformed cells. Thesis, University of Leiden, The Netherlands.:
    [Google Scholar]
  3. Bomhoff G. H., Klapwijk P. M., Kester H.C.M., Schilperoort R. A., Hernalsteens J. P., Schell J. 1976; Octopine and nopaline: synthesis and breakdown genetically controlled by a plasmid of Agrobacterium tumefaciens. Molecular and General Genetics 145:177–181
    [Google Scholar]
  4. Chilton M. D., Farrand S. K., Eden F., Currier T. C., Bendich A. T., Gordon M. P., Nester E. W. 1975; Is there foreign DNA in crown gall tumor DNA ?. In The Modification of the Information Content of Plant Cells, pp. 297–311 Markham R., Davies D. R., Hopwood D. A., Horne R. W. Edited by Amsterdam :: North Holland/American Elsevier.;
    [Google Scholar]
  5. Dons J.J.M. 1975 Crown gall- a plant tumor. Investigations on the nuclear DNA content and on the presence of Agrobacterium tumefaciens and phage PS8 DNA in crown gall cells. Thesis, University of Leiden, The Netherlands.:
    [Google Scholar]
  6. Drlica K. A., Kado C. I. 1975; Crown gall tumors : are bacterial nucleic acids involved ?. Bacteriological Reviews 39:186–196
    [Google Scholar]
  7. Engler G., Holsters M., Van Montagu M., Schell J., Hernalsteens J. P., Schilperoort R. A. 1975; Agrocin 84 sensitivity, a plasmid determined property. Molecular and General Genetics 138:345–349
    [Google Scholar]
  8. Goldmann A., Thomas D. W., Morel G. 1969; Sur la structure de la nopaline metabolite anormal de certainestumeurs de crown gall. Comptes rendus hebdomadaire des séances de l̓Academie des sciences 268:852–854
    [Google Scholar]
  9. Goldmann-MÉnagÉ A. 1970 Recherchessur la métabolisme azoté des tissus de Crown gall cultivés ̒in vitro̓. Thesis, Université de Paris, France.:
    [Google Scholar]
  10. Jubier M. F. 1972; Degradation of lysopine by an inducible membrane-bound oxidase in Agrobacterium tumefaciens. FEBS Letters 28:129–132
    [Google Scholar]
  11. Klapwijk P. M., De Jonge A.J.R., Schilperoort R. A., Rorsch A. 1975; An enrichment technique for auxotrophs of Agrobacterium tumefaciens using a combination of carbenicillin and lysozyme. Journal of General Microbiology 91:177–182
    [Google Scholar]
  12. Larebeke N., Van Engler G., Holsters M., Van Den Elsacker S., Zaenen I., Schilperoort R. A., Schell J. 1974; Large plasmid in Agrobacterium tumefaciens essential for crown gall inducing ability. Nature; London: 252169–170
    [Google Scholar]
  13. Larebeke N., Van Genetello CH., Schell J., Schilperoort R. A., Hermans A. K., Hernalsteens J. P., Van Montagu M. 1975; Acquisition of tumor inducing ability by non-oncogenic Agrobacteria as a result of plasmid transfer. Nature; London: 255:742–743
    [Google Scholar]
  14. Ledeboer A. M., Krol A.J.M., Dons J.J.M., Spier F., Schilperoort R. A., Zaenen I., Van Larebeke N., Schell J. 1976; On the isolation of T.I. plasmid from Agrobacterium tumefaciens. Nucleic Acids Research 3:449–464
    [Google Scholar]
  15. Lin E.C.C., Lerner S. A., Jorgensen S. E. 1962; A method for isolating constitutive mutants for carbohydrate catalyzing enzymes. Biochimica et biophysica acta 60:422–424
    [Google Scholar]
  16. Lioret C. 1956; Sur la mise en évidence d̓un acide aminé non identifié particulier aux tissus de crown-gall. Bulletin de la Société frangaise de physiologie végétale 2:76
    [Google Scholar]
  17. Lippincott J. A., Lippincoit B. B. 1975; The genus Agrobacterium and plant tumorigenesis. Annual Review of Microbiology 29:377–405
    [Google Scholar]
  18. MÉnagÉ A., Morel G. 1964; Sur la présence d̓octopinedans les tissus de crown-gall. Comptes rendus hebdomadaire des seances de l̓Académie des sciences 259:4795–4796
    [Google Scholar]
  19. MÉnagÉ A., Morel G. 1965; Sur la présence d̓uneacide aminé nouveau dans les tissus de crown-gall. Comptes rendus hebdomadaire des séances de l̓Académie des sciences 261:2001–2002
    [Google Scholar]
  20. Morel G. 1971; Déviations du métabolisme azote des tissus de crown gall. In Les Cultures de Tissus de Plantes pp. 463–471 Colloques Internationaux du C.N.R.S. Morel G. Edited by Paris:: Editions de l̓Institut National de la RechercheAgronomique.;
    [Google Scholar]
  21. Petit A., Delhaye S., TempÉ G., Morel G. 1970; Recherchessur les guanidines des tissus de crown gall. Mise en évidence d̓une relation biochimiquespecifique entre les souches d̓ Agrobacterium tumefaciens et les tumeurs qu̓elles induisent. Physiologie Végétale 8:205–213
    [Google Scholar]
  22. Schell J. 1975; The role of plasmids in crown-gall formation by Agrobacterium tumefaciens. . In Genetic Manipulations with Plant Material pp. 163–181 Ledoux L. Edited by New York:: Plenum Press.;
    [Google Scholar]
  23. Schilperoort R. A., Bomhoff G. H. 1975; Crown gall: a model for tumor research and genetic engineering. In Genetic Manipulations with Plant Material pp. 141–162 Ledoux L. Edited by New York:: Plenum Press.;
    [Google Scholar]
  24. Van Thoai N., Robin Y. 1961; Métabolisme des dérivés guanidylés. IX. Biosynthèse de l̓octopine: étude du méchanisme de la réaction et de quelques propriétés de l̓octopine synthetase. Biochimica et biophysica acta 52:221–233
    [Google Scholar]
  25. Vervliet G., Holsters M., Teuchy H., Van Montagu M., Schell J. 1975; Characterization of different plaque forming and defective temperate phages in Agrobacterium strains. Journal of General Virology 26:33–48
    [Google Scholar]
  26. Watson B., Currier T. C., Gordon M. P., Chilton M. D., Nester E. W. 1975; Plasmid required for virulence of Agrobacterium tumefaciens. Journal of Bacteriology 123:255–264
    [Google Scholar]
  27. Yamada S., Itano H. A. 1966; Phenantrene quinone as an analytical reagent for arginine and other mono-substituted guanidines. Biochimica et biophysica acta 130:538–540
    [Google Scholar]
  28. Zaenen I., Van Larebeke N., Teuchy H., Van Montagu M., Schell J. 1974; Supercoiled circular DNA in crown gall inducing Agrobacterium strains. Journal of Molecular Biology 86:109–127
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-96-1-155
Loading
/content/journal/micro/10.1099/00221287-96-1-155
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error