1887

Abstract

SUMMARY: The interaction of amphotericin B methyl ester (AME) with protoplasts of was measured indirectly by following the incorporation of [U-C]phenylalanine into the acid-insoluble material. The inhibitory effects of AME at the minimum inhibitory concentration were prevented by the addition of 85 m-KCl and 45 m-MgCl, as shown by Liras & Lampen (1974) for . In , pretreatment of the yeast before antibiotic addition was unnecessary. KCl and MgCl did not prevent AME from binding to the protoplast membrane. This interaction was reversed by incubating the protoplasts in the presence of the protecting salts.

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/content/journal/micro/10.1099/00221287-96-1-117
1976-09-01
2021-10-17
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