1887

Abstract

SUMMARY: Phagocytes obtained from fresh human buffy coat (predominantly polymorpho-nuclear phagocytes) or from human buffy coat which had been incubated on a glass surface for 1 to 3 days (predominantly mononuclear phagocytes) were allowed to ingest gonococci, and then incubated with penicillin. More intracellular gonococci were killed at high than at low penicillin concentrations, indicating that penicillin penetrated the phagocytes. This was supported by autoradiography experiments with radiolabelled penicillin.

A pilated, small-colony-forming gonococcal strain survived and multiplied for at least 15 h in polymorphonuclear phagocytes which were incubated with penicillin at the optimum concentration for killing the extracellular bacteria but not the intracellular ones; whereas a non-pilated, large-colony-forming strain survived for only 10 h. The former strain survived for at least 6 h in similar experiments with mononuclear phagocytes. Intracellular survival and growth may be an important facet of the pathogenicity of gonococci.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-95-2-353
1976-08-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/95/2/mic-95-2-353.html?itemId=/content/journal/micro/10.1099/00221287-95-2-353&mimeType=html&fmt=ahah

References

  1. Cole J., Brostoff J. 1975; Intracellular killing of Listeria monocytogenes by activated macrophages (Mackaness system) is due to antibiotic. Nature; London: 256515–517
    [Google Scholar]
  2. Farzadeghan H., Roth I. L. 1975; Scanning electron microscopy and freeze-etching of gonorrhoeal urethral exudate. British Journal of Venereal Disease 51:83–91
    [Google Scholar]
  3. Frost A. L., Smith H., Witt K., Keppie J. 1972; The chemical basis of the virulence of Brucella abortus. X. A surface virulence factor which facilitates intracellular growth of Brucella abortus in bovine phagocytes. British Journal of Experimental Pathology 53:587–596
    [Google Scholar]
  4. Gibbs D. L., Roberts R. B. 1975; The interaction in vitro between human polymorphonuclear leukocytes and Neisseria gonorrhoeae cultivated in the chick embryo. Journal of Experimental Medicine 141:155–171
    [Google Scholar]
  5. Holmes B., Quie P. G., Windhorst D. B., Pollara B., Good R. A. 1966; Protection of phagocytosed bacteria from the killing action of antibiotics. Nature; London: 2101131–1132
    [Google Scholar]
  6. Kellogg D. S. Jr Peacock W. L. Jr Brown L., Pirkle C. I. 1963; Neisseria gonorrhoeae. I. Virulence genetically linked in clonal variation. Journal of Bacteriology 85:1274–1279
    [Google Scholar]
  7. Kellogg D. S. Jr cohen I. R., Norins L. C., Schroeter A. L., Reising G. 1968; Neisseria gonorrhoeae. II. Colonial variation and pathogenicity during 35 months in vitro. . Journal of Bacteriology 96:596–605
    [Google Scholar]
  8. Kenny C. P., Aris B. J. 1969; The infection of tissue cells in culture with Neisseria gonorrhoeae. . Canadian Journal of Public Health 60:34
    [Google Scholar]
  9. Mandell G. L. 1973; Interaction of intraleucocytic bacteria and antibiotics. Journal of Clinical Investigation 52:1673–1679
    [Google Scholar]
  10. Novotny P., Short J. A., Walker P. D. 1975; An electron-microscope study of naturally occurring and cultured cells of Neisseria gonorrhoeae. . Journal of Medical Microbiology 8:413–427
    [Google Scholar]
  11. Ota F., Morita J., Yoshida N., Ashton F., Diena B. 1975; Studies on gonococcal infection. I. Electron microscope studies on phagocytosis of Neisseria gonorrhoeae by macrophages. Japanese Journal of Microbiology 19:149–155
    [Google Scholar]
  12. Ovcinnikov N. M., Delektorskij V. V. 1971; Electron microscope studies of gonococci in the urethral secretions of patients with gonorrhoea. British Journal of Venereal Disease 47:419–439
    [Google Scholar]
  13. Ovcinnikov N. M., Bohenek A. A., Litvak P. B. 1934; Phagocytosis or endocytobiotics, its significance in gonorrhoea. Urologiya 11:84–88
    [Google Scholar]
  14. Ovcinnikov N. M., Delektorskij V. V., Afansev B. A. 1974; Electron microscopy of gonococci in the urethral secretions of patients with gonorrhoea treated with penicillin and erythromycin. British Journal of Venereal Disease 50:179–194
    [Google Scholar]
  15. Patterson R. J., Youmans G. P. 1970; Multiplication of Mycobacterium tuberculosis within normal and ‘immune’ mouse macrophages cultivated with and without streptomycin. Infection and Immunity 1:30–40
    [Google Scholar]
  16. Rogers A. W. 1973 Techniques of Autoradiography, 2nd ed.. Amsterdam: Elsevier.;
    [Google Scholar]
  17. Smith H. 1968; Biochemical challenge of microbial pathogenicity. Bacteriological Reviews 32:164–184
    [Google Scholar]
  18. Thayer J. D., Perry M. I., Field F. W., Garson W. 1956; Failure of penicillin, chloramphenicol, erythromycin and novobiocin to kill phagocytosed gonococci in tissue culture. Antibiotics Annual513–517
    [Google Scholar]
  19. Thomas D. W., Hill J. C., Tyeryar F. J. Jr 1973; Interaction of gonoccoci with phagocytic leukocytes from men and mice. Infection and Immunity 8:98–104
    [Google Scholar]
  20. Veale D. R., Smith H., Witt K. 1975a; Penetration of penicillin into human phagocytes containing gonococci. Lancet i:306–308
    [Google Scholar]
  21. Veale D. R., Smith H., Witt K., Marshali R. B. 1975b; Differential ability of colonial types of Neisseria gonorrhoeae to produce infection and an inflammatory response in subcutaneously perforated plastic chambers in guinea-pigs and rabbits. Journal of Medical Microbiology 8:325–335
    [Google Scholar]
  22. Veale D. R., Witt K., Smith H. 1975c; Penetration of penicillin into human phagocytes containing gonococci: intracellular survival at minimal concentrations of antibiotic. Proceedings of the Society for General Microbiology 2:75
    [Google Scholar]
  23. Ward M. E., Glynn A. A., Watt P. J. 1972; The fate of gonococci in polymorphonuclear leukocytes: an electron microscope study of the natural disease. British Journal of Experimental Pathology 53:289–294
    [Google Scholar]
  24. Watt P. J. 1970; The fate of gonococci in polymorphonuclear leukocytes. Journal of Medical Microbiology 3:501–509
    [Google Scholar]
  25. Witt K., Veale D. R., Smith H. 1976; Resistance to ingestion and digestion of Neisseria gonorrhoeae by phagocytes of human buffy coat. Journal of Medical Microbiology 9:1–12
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-95-2-353
Loading
/content/journal/micro/10.1099/00221287-95-2-353
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error