1887

Abstract

SUMMARY: Two hundred and seventy Gram-negative strains, representing aquatic members of the genus , were isolated and compared with 48 related clinical isolates and reference strains from a variety of genera. For each isolate, a total of 96 coded characters derived from 89 characteristics was determined using morphological, physiological, nutritional and biochemical features, in addition to sensitivities to several antibiotics and inhibitory agents. The data were analysed by computer to obtain a simple matching coefficient for each pair of strains. Clustering was performed by the unweighted pair-group method of association.

Two major phenons were formed which excluded the oxidase-positive, motile or facultatively anaerobic strains. Within each phenon, three ‘subphenons’ were delimited. The two phenons, comprising 291 isolates, were tentatively differentiated at the species level, while their shared characteristics indicated that both phenons should be included in the genus Phenon 2 contained most of the clinical isolates and corresponded to the type species as described originally by Baumann, Doudoroff & Stanier (1968). Phenon 1 was composed almost entirely of aquatic isolates and may prove to represent a second species of a less biochemically-active nature.

Distinguishing characters have been suggested as diagnostic criteria for the differentiation of these two phenons.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-95-2-220
1976-08-01
2021-05-13
Loading full text...

Full text loading...

/deliver/fulltext/micro/95/2/mic-95-2-220.html?itemId=/content/journal/micro/10.1099/00221287-95-2-220&mimeType=html&fmt=ahah

References

  1. Ayers S. H., Rupp P., Johnson W. T. 1919 A Study of the Alkali-forming Bacteria in Milk. United States Department of Agriculture Bulletin 82 Washington, U.S.A.: Department of Agriculture;
    [Google Scholar]
  2. Baumann P. 1968; Isolation of Acinetobacter from soil and water. Journal of Bacteriology 96:39–42
    [Google Scholar]
  3. Baumann P., Doudoroff M., Stanier R. Y. 1968; A study of the Moraxella group. II. Oxidasenegative species (genus Acinetobacter). Journal of Bacteriology 95:1520–1541
    [Google Scholar]
  4. Beijerinck M. W. 1911; Über Pigmentbildung bei Essigbakterien. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung II) 29:169–176
    [Google Scholar]
  5. Bennett E. A. 1969; A Study of the Distribution of Heterotrophic Bacteria in the Great Lakes. I. The Heterotrophs in Lake Water. Ontario, Canada: Bacteriology Branch, Division of Laboratories, Ontario Water Resources Commission;
    [Google Scholar]
  6. Billing E. 1955; Studies on a soap tolerant organism-a new variety of Bacterium anitratum. Journal of General Microbiology 13:252–260
    [Google Scholar]
  7. Board R. G., Holding A. J. 1960; The utilization of glucose by aerobic Gram-negative bacteria. Journal of Applied Bacteriology 23xi–xii
    [Google Scholar]
  8. Bövre K. 1967; Transformation and DNA base composition in taxonomy, with special reference to recent studies in Moraxella and Neisseria. Acta pathologica et microbiologica scandinavica 69:123–144
    [Google Scholar]
  9. Brodie J., Henderson A. 1964; Further observations on Mima polymorpha and Achromobacter (Bacterium anitratum). Journal of Clinical Pathology 17:513–516
    [Google Scholar]
  10. Brooks K., Sodeman T. 1974; Clinical studies on a transformation test for identification of Acineto-bacter (Mima and Herellea). Applied Microbiology 271023–1026
    [Google Scholar]
  11. Burrows S., King M. J. 1966; Meningitis due to Mima polymorpha. American Journal of Clinical Pathology 46:234–238
    [Google Scholar]
  12. Chapman G. S. 1952; A simple method for making multiple tests on a micro-organism. Journal of Bacterio-logy 63147
    [Google Scholar]
  13. Christensen W. B. 1946; Urea decomposition as a means of differentiating Proteus and para colon cultures from each other and from Salmonella and Shigella types. Journal of Bacteriology 54:461–466
    [Google Scholar]
  14. Colwell R. R., Mandel M. 1964; Adansonian analysis and deoxyribonucleic acid base composition of some Gram-negative bacteria. Journal of Bacteriology 87:1412–1422
    [Google Scholar]
  15. De ley J. 1968; DNA base composition and taxonomy of some Acinetobacter strains. Antonie van Leeuwenhoek 34:109–114
    [Google Scholar]
  16. Duguid J. P. 1951; The demonstration of bacterial capsules and slime. Journal of Pathogenic Bacteriology 63:673–685
    [Google Scholar]
  17. Ferguson W. W., Roberts L. F. 1950; A bacteriological and serological study of organisms B5W (Bacterium anitratum.). Journal of Bacteriology 59:171–183
    [Google Scholar]
  18. Goldberg A. S., Ronis B. J., Ronis M. L. 1966; Mima polymorpha as a cause of otitis externa. Report of a case. Archives of Otolaryngology 83:29–30
    [Google Scholar]
  19. Henriksen S. D. 1973; Moraxella, Acinetobacter and the Mimeae. Bacteriological Reviews 37:522–561
    [Google Scholar]
  20. Hugh R., Reese R. 1967; Designation of the type strain for Bacterium anitratum (Schaub and Hauber). International Journal of Systematic Bacteriology 17245–254
    [Google Scholar]
  21. Hugh R., Reese R. 1968; A comparison of 120 strains of Bacterium anitratum (Schaub and Hauber) with the type strain of this species. International Journal of Systematic Bacteriology 18:207–229
    [Google Scholar]
  22. Johnson J. L., Anderson R. S., Ordal E. J. 1970; Nucleic acid homologies among oxidase-negative Moraxella species. Journal of Bacteriology 101:568–573
    [Google Scholar]
  23. Judicial Commission 1971; Minutes of meeting of the Judicial Commission, Mexico City, Mexico. International Journal of Systematic Bacteriology 21213–214
    [Google Scholar]
  24. Juni E. 1972; Interspecies transformation of Acinetobacter: genetic evidence for a ubiquitous genus. Journal of Bacteriology 112:917–931
    [Google Scholar]
  25. Kun E., Abood L. G. 1949; Biochemical properties of succinoxidase from Salmonella aertrycke. Journal of Biological Chemistry 180:813–823
    [Google Scholar]
  26. Langhof H., Franken E. 1964; Pseudogonorrhoeal vulvovaginitis due to penicillin-resistant Mimeae. Deutsches Gesundheitswesen 19:574–575
    [Google Scholar]
  27. Lederberg J., Lederberg E. M. 1952; Replica plating and indirect selection of bacterial mutants. Journal of Bacteriology 63:399–406
    [Google Scholar]
  28. Leifson E., Mandel M. 1966; The DNA base composition of a flagellar mutant of Comamonas terrigena atcc8461. Antonie van Leeuwenhoek 32:57–59
    [Google Scholar]
  29. Manual Of Microbiological Methods 1957; Prepared by the Society of American Bacteriologists. New York: McGraw-Hill;
  30. Marmur J. 1961; A procedure for the isolation of DNA from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  31. Mcclung L. S., Heidenreich P., Toabe R. 1946; A medium for the Nagler plate reactions for the identification of certain Clostridia. Journal of Bacteriology 51:751–752
    [Google Scholar]
  32. Olafsson M., Lee Y. C., Abernathy T. J. 1958; Mima polymorpha meningitis. Report of a case and review of the literature. New England Journal of Medicine 258:465–470
    [Google Scholar]
  33. Opsahl R.Jr 1961; Bacterium anitratum (Acinetobacter anitratum) isolated from a case with a cerebral abscess and purulent meningitis. Acta pathologica et microbiologica scandinavica 51:72–74
    [Google Scholar]
  34. Pintér M., Bende I. 1967; Computer analysis of Acinetobacter Iwoffi (Moraxella Iwofft) and Acinetobacter anitratus (.Moraxella glucidolytica) strains. Journal of General Microbiology 46:267–272
    [Google Scholar]
  35. Reinarz J. A. 1965; The potential role of inhalation therapy equipment in nosocomial pulmonary infection. Journal of Clinical Investigation 44831–839
    [Google Scholar]
  36. Rhodes M. E. 1959; The characterization of Pseudomonas fluorescens. Journal of General Microbiology 21:221–263
    [Google Scholar]
  37. Rogers K. B., Taylor J. 1961; Laboratory diagnosis of gastroenteritis due to Escherichia coli. Bulletin of the World Health Organization 2459–71
    [Google Scholar]
  38. Rosebury T. 1962 Micro-organisms Indigenous to Man. New York: McGraw-Hill;
    [Google Scholar]
  39. Samuels S. B., Pittman B. P., Tatum H. W., Cherry W. B. 1972; Report on a study set of Moraxella and allied bacteria. International Journal of Systematic Bacteriology 2219–38
    [Google Scholar]
  40. Schaub I., Hauber F. E. 1948; A biochemical and serological study of a group of unidentifiable Gramnegative bacilli from human sources. Journal of Bacteriology 56:379–385
    [Google Scholar]
  41. Schildkraut C., Lifson S. 1965; Dependence of the melting temperature of DNA on salt concentration. Biopolymers 3:195
    [Google Scholar]
  42. Seyfried P. L. 1973; Sampling bacteria in Lake Ontario and the Toronto Harbour. Proceedings of the 16th Conference of Great Lakes Research pp. 163–182 Ann Arbor, Michigan, U.S.A.: International Associa-tion for Great Lakes Research;
    [Google Scholar]
  43. Shklair I. L. 1965; Mima-Herellea organisms in the saliva of healthy male naval recruits. Bacteriological Proceedings 49: abstract N60
    [Google Scholar]
  44. Simmons J. S. 1926; A culture medium for differentiating organisms of typhoid-colon-aerogenes groups and for isolation of certain fungi. Journal of Infectious Diseases 39:209–217
    [Google Scholar]
  45. Sneath P.H.A., Sokal R. R. 1973 Numerical Taxonomy. San Francisco: W. H. Freeman;
    [Google Scholar]
  46. Sokal R. R., Michener C. D. 1958; A statistical method for evaluating systematic relationships. Kansas University Science Bulletin 38:1409–1438
    [Google Scholar]
  47. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. Journal of General Microbiology 49:159–271
    [Google Scholar]
  48. Stumpf P. K. 1952; Phosphate assimilation in higher plants. In Phosphorus Metabolism 2 pp. 29–67 McElroy W. D., Baltimore B. Glass. Edited by Johns Hopkins;
    [Google Scholar]
  49. Taindel C., Oproiu L., Burlacu G. 1971; Acute meningitis in a child caused BY Acinetobacter. Viata Medicala 18:67–69
    [Google Scholar]
  50. Thornley M. J. 1960; The differentiation of Pseudomonas from other Gram-negative bacteria on the basis of arginine metabolism. Journal of Applied Bacteriology 2337–52
    [Google Scholar]
  51. Thornley M. J. 1967; A taxonomic study of Acinetobacter and related genera. Journal of General Microbiology 49:211–257
    [Google Scholar]
  52. Warskow A. L., Juni E. 1972; Nutritional requirements of Acinetobacter strains isolated from soil, water, and sewage. Journal of Bacteriology 112:1014–1016
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-95-2-220
Loading
/content/journal/micro/10.1099/00221287-95-2-220
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error