RT Journal Article SR Electronic(1) A1 Mccullough, Clare H. R. A1 Dee, JenniferYR 1976 T1 Defined and Semi-defined Media for the Growth of Amoebae of Physarum polycephalum JF Microbiology, VO 95 IS 1 SP 151 OP 158 DO https://doi.org/10.1099/00221287-95-1-151 PB Microbiology Society, SN 1465-2080, AB SUMMARY: Amoebae of the true slime mould Physarum polycephalum were cultured in two fully-defined liquid media containing amino acids, glucose, three vitamins and a buffered salts solution. Absolute requirements were demonstrated for methionine, haematin, thiamine and biotin, all of which were known to be specific requirements of the plasmodial stage. Methods are described for large-scale culture in three semi-defined media., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-95-1-151