1887

Abstract

SUMMARY: Conditions are described for the isolation from the rumen and subsequent growth of six species of cellulolytic protozoa: and The protozoa were grown in an atmosphere of 95% nitrogen plus 5% carbon dioxide, or pure carbon dioxide, in a potassium phosphate-rich medium containing cysteine, sometimes 10% prepared fresh rumen fluid, and daily additions of powdered dried grass. Population densities of 10 to 6000 protozoa/ml (depending on the species) were obtained in cultures that were diluted with fresh medium twice each week. Extracts of these protozoa digested a [C]cellulose preparation, liberating soluble C-labelled compounds.

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/content/journal/micro/10.1099/00221287-95-1-144
1976-07-01
2021-10-24
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References

  1. Abou Akkada A. R., Bartley E. E., Berube R., Fina L. R., Meyer R. M., Hendricks D., Julius F. 1968; Simple method to remove completely ciliate protozoa of adult ruminants. Applied Microbiology 16:1475–1477
    [Google Scholar]
  2. Coleman G. S. 1958; Maintenance of oligotrich protozoa from the sheep rumen in vitro. Nature; London: 1821104–1105
    [Google Scholar]
  3. Coleman G. S. 1960; The cultivation of sheep oligotrich protozoa in vitro. Journal of General Microbiology 22:555–563
    [Google Scholar]
  4. Coleman G. S. 1969a; The cultivation of the rumen ciliate Entodinium simplex. Journal of General Microbiology 57:81–90
    [Google Scholar]
  5. Coleman G. S. 1969b; The metabolism of starch, maltose, glucose and some other sugars by the rumen ciliate Entodinium caudataum. Journal of General Microbiology 57:303–332
    [Google Scholar]
  6. Coleman G. S. 1972; The metabolism of starch, glucose, amino acids, purines, pyrimidines and bacteria by the rumen ciliate Entodinium simplex. Journal of General Microbiology 71:117–131
    [Google Scholar]
  7. Coleman G. S., Davies J. I., Cash M. A. 1972; The cultivation of the rumen ciliates Epidinium ecaudatum caudatum and Polyplastron multivesiculatum in vitro. Journal of General Microbiology 73:509–521
    [Google Scholar]
  8. Dawson R.M.C., Hemington N. 1974; Digestion of grass lipids and pigments in the sheep rumen. British Journal of Nutrition 32:327–340
    [Google Scholar]
  9. Dogiel V. A. 1925; Nouveaux infusoires de la famille des Ophryoscolecides parasites d̓antilopes africaines. Annales de parasitologies humaine et comparée 3:116–142
    [Google Scholar]
  10. Dogiel V. A. 1927; Monographic der Familie Ophryoscolecidae. Archiv für Protistenkunde 59:1–227
    [Google Scholar]
  11. Eadie J. M. 1962; Inter-relationships between certain rumen protozoa. Journal of General Microbiology 29:579–588
    [Google Scholar]
  12. Eadie J. M. 1967; Studies on the ecology of certain rumen ciliate protozoa. Journal of General Microbiology 49:175–194
    [Google Scholar]
  13. Fiorentini A. 1889 Intorno aiProstisto dello Stomacho dei Bovino. Pavia:: Fusi.;
    [Google Scholar]
  14. Hungate R. E. 1942; The culture of Eudiplodinium neglectum, with experiments on the digestion of cellulose. Biological Bulletin. Marine Biological Laboratory, Woods Hole, Massachussetts 83:303–319
    [Google Scholar]
  15. Hungate R. E. 1943; Further experiments on the cellulose digestion by the protozoa in the rumen of cattle. Biological Bulletin. Marine Biological Laboratory, Woods Hole, Massachusetts 84:157–163
    [Google Scholar]
  16. Jermyn M. A. 1955; Cellulose and hemicelluloses. In Modern Methods of Plant Analysis 2 pp. 197–225 Paech K., Tracey M. V. Edited by Berlin:: Springer-Verlag.;
    [Google Scholar]
  17. Kofoid C. A., Maclennan R. F. 1932; Ciliates from Bos indicus Linn. 2. A revision of Diplodinium Schuberg. University of California Publications in Zoology 37:53–152
    [Google Scholar]
  18. Tappeiner H. 1884; Untersuchungen liber die Garung der Cellulose, inbesondere iiber deren Losung im Darmkanal. Zeitschrift für Biologie 20:52–134
    [Google Scholar]
  19. Trier H. J. 1926; Der Kohlenhydratstoffwechsel der Panseninfusorien und die Bedeutung der grünen Pflanzenteile für diese Organismen. Zeitschrift für vergleichende Physiologie 4:305–330
    [Google Scholar]
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