SUMMARY: Conditions are described for the isolation from the rumen and subsequent growth of six species of cellulolytic protozoa: and The protozoa were grown in an atmosphere of 95% nitrogen plus 5% carbon dioxide, or pure carbon dioxide, in a potassium phosphate-rich medium containing cysteine, sometimes 10% prepared fresh rumen fluid, and daily additions of powdered dried grass. Population densities of 10 to 6000 protozoa/ml (depending on the species) were obtained in cultures that were diluted with fresh medium twice each week. Extracts of these protozoa digested a []cellulose preparation, liberating soluble C-labelled compounds.


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