RT Journal Article SR Electronic(1) A1 Taylor, I. J. A1 Anthony, C.YR 1976 T1 Acetyl-CoA Production and Utilization during Growth of the Facultative Methylotroph Pseudomonasam1 on Ethanol, Malonate and 3-Hydroxybutyrate JF Microbiology, VO 95 IS 1 SP 134 OP 143 DO https://doi.org/10.1099/00221287-95-1-134 PB Microbiology Society, SN 1465-2080, AB SUMMARY: In Pseudomonas ami, conversion of 3-hydroxybutyrate to acetyl-CoA is mediated by an inducible 3-hydroxybutyrate dehydrogenase, an acetoacetate: succinate coenzyme A transferase (specific for succinyl-CoA) and an inducible β-ketothiolase. Ethanol is oxidized to acetate by the same enzymes as are involved in methanol oxidation to formate. An inducible acetyl-CoA synthetase has been partially purified and characterized; it is essential for growth only on ethanol, malonate and acetate plus glyoxylate, as shown by the growth characteristics of a mutant (ict54) lacking this enzyme. Free acetate is not involved in the assimilation of acetyl-CoA, and hydroxypyruvate reductase is not involved in the oxidation of acetyl-CoA to glyoxylate during growth on 3-hydroxybutyrate. A mutant (ict51), lacking malate synthase activity, has been isolated and its characteristics indicate that this activity is normally essential for growth of Pseudomonas ami on ethanol, malonate and 3-hydroxybutyrate, but not for growth on other substrates such as pyruvate, succinate and C1 compounds. The growth properties of a revertant ( ict51r) and of a mutant lacking malyl-CoA lyase (pct57) indicate that an alternative route must exist for assimilation of compounds metabolized exclusively by way of acetyl-CoA., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-95-1-134