Effect of Growth Temperature on the Cryopreservation of Prototheca Free

Abstract

Summary: The temperature at which spp. were grown determined their response to freezing to −196 °C and subsequent thawing. Cells cultured at 35 °C were the most sensitive to freezing injury; at lower growth temperatures, resistance to freezing damage was seen. At all culture temperatures examined, the freezing tolerance varied with the age of the culture.

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1976-06-01
2024-03-28
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