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Summary: Utilization of acetate by four strains of Butyrivibrio fibrisolvens was influenced by the composition of their growth medium. Growth-limiting glucose concentrations, low availability of CO2 and the presence of sodium lactate all stimulated acetate uptake by three strains. The type strain, d 1, utilized acetate if the concentration of acetate added to the medium was at least 15 μmol ml−1. In batch culture, all strains produced acetate before entering a phase of acetate uptake. Continuous-culture studies with one strain showed that acetate uptake was dependent upon growth rate. The amount of n-butyrate produced in batch or continuous culture was closely linked to the amount of acetate taken up.
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