1887

Abstract

Summary: Four independent porphobilinogen-accumulating mutants of were isolated by selecting for dwarf colony formation on neomycin agar media. Cell-free extracts of the parent strain, but not of the mutants, were able to convert 5-aminolaevulinic acid or porphobilinogen to porphyrins. The results indicated that the mutants were deficient in uroporphyrinogen I synthase (EC.4.3.1.8) activity: these are the first mutants of this type reported in . Mapping of the locus (for uroporphyrinogen I synthase) by F-mediated conjugation and by P22-mediated transduction showed the gene sequence .

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1976-06-01
2021-10-23
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