1887

Abstract

Summary: Four independent porphobilinogen-accumulating mutants of were isolated by selecting for dwarf colony formation on neomycin agar media. Cell-free extracts of the parent strain, but not of the mutants, were able to convert 5-aminolaevulinic acid or porphobilinogen to porphyrins. The results indicated that the mutants were deficient in uroporphyrinogen I synthase (EC.4.3.1.8) activity: these are the first mutants of this type reported in . Mapping of the locus (for uroporphyrinogen I synthase) by F-mediated conjugation and by P22-mediated transduction showed the gene sequence .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-94-2-359
1976-06-01
2024-11-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/94/2/mic-94-2-359.html?itemId=/content/journal/micro/10.1099/00221287-94-2-359&mimeType=html&fmt=ahah

References

  1. Bauer J. D., Ackermann P. G., Toro G. 1968 Bray’s Clinical Laboratory Methods, 7th edn.. St Louis, Missouri, U.S.A.: Mosby;
    [Google Scholar]
  2. Berek L., Miczak A., Ivanovics G. 1974; Mapping the Δ-aminolevulinic acid dehydrase and porphobilinogen deaminase loci in Bacillus subtilis. Molecular and General Genetics 132:233–239
    [Google Scholar]
  3. Bogorad L. 1958; The enzymatic synthesis of porphyrins from porphobilinogen. I. Uroporphyrin. Journal of Biological Chemistry 233:501–509
    [Google Scholar]
  4. Casse F., Pascal M. C., Chippaux M., Ratouchniak J. 1972; Mapping of the chlB gene in Salmonella typhimurium LT2. Molecular and General Genetics 119:67–70
    [Google Scholar]
  5. Cookson G. H., Rimington C. 1953; Porphobilinogen: chemical constitution. Nature, London 171:875–876
    [Google Scholar]
  6. Cookson G. H., Rimington C. 1954; Porphobilinogen. Biochemical Journal 57:476–484
    [Google Scholar]
  7. Cox R., Charles H. P. 1973; Porphyrin-accumulating mutants of Escherichia coli. Journal of Bacteriology 113:122–132
    [Google Scholar]
  8. Dresel E. I. B., Rimington C., Tooth C. E. 1956; Determination of urinary uroporphyrin by a direct extraction method. Scandinavian Journal of Clinical and Laboratory Investigation 8:73–78
    [Google Scholar]
  9. Eriksen L. 1958; Paper chromatographic separation of the coproporphyrin isomers I and III. Scandinavian Journal of Clinical and Laboratory Investigation 10:319–322
    [Google Scholar]
  10. Falk J. E. 1964 Porphyrins and metallo-porphyrins. New York: American Elsevier Publications;
    [Google Scholar]
  11. Falk J. E., Dresel E. I. B., Rimington C. 1953; Porphobilinogen as a porphyrin precursor, and interconversion of porphyrins, in a tissue system. Nature; London: 172292–294
    [Google Scholar]
  12. Gajdos A., Gajdos-Török M. 1969 Porphyrines et porphyries. Paris: Masson;
    [Google Scholar]
  13. Hatch T., Lascflles J. 1972; Accumulation of porphobilinogen and other pyrroles by mutant and wild-type Rhodopseudomonas spheroides: regulation by heme. Archives of Biochemistry and Biophysics 150:147–153
    [Google Scholar]
  14. Herbert D., Pinsent J. 1948; Crystalline bacterial catalase. Biochemical Journal 43:193–202
    [Google Scholar]
  15. Ivanovics G., Koczka S. 1952; Zusammenhang zwischen antibakterieller Wirkung des Mesohaematins und der Bakterien-Zellstruktur. Acta physiologica Academiae scientiarum hungariae 3:441–457
    [Google Scholar]
  16. Jordan P. M., Shemin D. 1973; Purification and properties of uroporphyrinogen I synthetase from Rhodopseudomonas spheroides. Journal of Biological Chemistry 248:1019–1024
    [Google Scholar]
  17. Kennedy G. Y. 1956; A new method for the extraction of uroporphyrin I from mollusc shells. Scandinavian Journal of Clinical and Laboratory Investigation 8:79–80
    [Google Scholar]
  18. Lascelles J. 1956; The synthesis of porphyrins and bacteriochlorophyll by cell suspensions of Rhodopseudomonas spheroides. Biochemical Journal 62:78–93
    [Google Scholar]
  19. Lascelles J. 1964 Tetrapyrrol Biosynthesis and its Regulation New York: Benjamin;
    [Google Scholar]
  20. Lascelles J., Hatch T. P. 1969; Bacteriochlorophyll and heme synthesis in Rhodopseudomonas spheroides: possible role of heme in regulation of the branched biosynthetic pathway. Journal of Bacteriology 98:712–720
    [Google Scholar]
  21. Layne E. 1957; Spectrophotometric and turbidimetric methods for measuring proteins. In Methods in Enzymology 3 pp. 447–454 Colowick S. P., Kaplan N. O. Edited by New York: Academic;
    [Google Scholar]
  22. Mauzerall D., Granick S. 1956; The occurrence and determination of Δ-aminolevulinic acid and porphobilinogen in urine. Journal of Biological Chemistry 219:435–446
    [Google Scholar]
  23. Perrin D. D., Armarego W. L. F., Perrin D. P. 1966 Purification of Laboratory Chemicals. Oxford: Pergamon;
    [Google Scholar]
  24. Porra R. J., Falk J. E. 1964; The enzymic conversion of coproporphyrinogen III into protoporphyrin IX. Biochemical Journal 90:69–75
    [Google Scholar]
  25. Powell K. A., Cox R., Mcconville M., Charles H. P. 1973; Mutations affecting porphyrin biosynthesis in Escherichia coli. Enzyme 16:65–73
    [Google Scholar]
  26. Rimington C. 1960; Spectral-absorption coefficients of some porphyrins in the Soret-Band region. Biochemical Journal 75:620–623
    [Google Scholar]
  27. Sanderson K. E. 1972; Linkage map of Salmonella typhimurium. Edition IV. Bacteriological Reviews 36:588–586
    [Google Scholar]
  28. Sanderson K. E., Demerec M. 1965; The linkage map of Salmonella typhimurium. Genetics 51:897–913
    [Google Scholar]
  29. Sǎsǎrman A., Chartrand P., Proschek R., Desrochers M., Tardif D., Lapointe C. 1975; Uroporphyrin-accumulating mutant of Escherichia coli KI2. Journal of Bacteriology 124:1205–1212
    [Google Scholar]
  30. Sǎsǎrman A., Sonea S. 1975; Two new hem loci in a cluster of respiratory genes of Enterobacteriaceae. In Proceedings of the First Intersectional Congress of the International Association of Microbiological Societies, Tokyo 1974 1 pp. 177–181 Hasegawa T. Edited by Tokyo: Science Council of Japan;
    [Google Scholar]
  31. Sǎsǎrman A., Surdeanu M., Szegli G., Horodniceanu T., Greceanu V., Dumitrescu A. 1968; Hemin-deficient mutants of Escherichia coli KI2. Journal of Bacteriology 96:570–572
    [Google Scholar]
  32. Tait G. H. 1968; General aspects of haem synthesis. In Porphyrins and Related Compounds pp. 19–34 Goodwin T. W. Edited by New York: Academic;
    [Google Scholar]
  33. Westall R. G. 1952; Isolation of porphobilinogen from the urine of a patient with acute porphyria. Nature; London: 170614–616
    [Google Scholar]
/content/journal/micro/10.1099/00221287-94-2-359
Loading
/content/journal/micro/10.1099/00221287-94-2-359
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error