1887

Abstract

Summary: strain 10050, grown anaerobically in the light on methanol, contained a methanol and formaldehyde dehydrogenase which could be coupled to phenazine methosulphate; an NAD-linked formaldehyde dehydrogenase which required GSH for activity; and an NAD-linked formate dehydrogenase. The specific activities of these enzymes varied in a non-coordinate manner when the organism was grown on different alcohols, formate or succinate. The affinity of the phenazine methosulphate linked methanol dehydrogenase for methanol was increased 10-fold if the cell-free extract was prepared and assayed in the absence of oxygen. Pulse-labelling experiments with [C]methanol and [C]bicarbonate indicated that fixation of carbon dioxide occurred via the ribulose diphosphate cycle and C + CO fixation reaction(s). No evidence was obtained for operation of a reduced C fixation sequence. This conclusion was borne out by the enzyme content of cell-free extracts of the organism.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-94-2-313
1976-06-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/94/2/mic-94-2-313.html?itemId=/content/journal/micro/10.1099/00221287-94-2-313&mimeType=html&fmt=ahah

References

  1. Anthony C., Zatman L. J. 1964; The microbial oxidation of methanol. The methanol oxidizing enzyme of Pseudomonas sp. M27. Biochemical Journal 96:808–812
    [Google Scholar]
  2. Bellion E., Hersh L. B. 1972; Methylamine metabolism in a Pseudomonas species. Archives of Biochemistry and Biophysics 153:368–374
    [Google Scholar]
  3. Cox R. B., Quayle J. R. 1975; The autotrophic growth of Micrococcus denitrificans on methanol. Biochemical Journal 150:569–571
    [Google Scholar]
  4. Dixon G. H., Kornberg H. L. 1959; Assay methods for key enzymes of the glyoxylate cycle. Biochemical Journal 72:3P
    [Google Scholar]
  5. Ferenci T., Strøm T., Quayle J. R. 1974; Purification and properties of 3-hexulose phosphate synthase and phospho-3-hexuloisomerase from Methylococcus capsulatus. Biochemical Journal 144:477–486
    [Google Scholar]
  6. Johnson P. A., Quayle J. R. 1964; Microbial growth on Cx compounds. 6. Oxidation of methanol, formaldehyde and formate by methanol-grown Pseudomonas ami. Biochemical Journal 93:281–290
    [Google Scholar]
  7. Knight M. 1962; The photometabolism of propionate by Rhodospirillum rubrum. Biochemical Journal 84:170–185
    [Google Scholar]
  8. Large P. J., Quayle J. R. 1963; Microbial growth on C1 compounds. 5. Enzyme activities in extracts of Pseudomonas AMI. Biochemical Journal 87:386–396
    [Google Scholar]
  9. Lawrence A. J., Kemp M. B., Quayle J. R. 1970; Synthesis of cell constituents by methane-grown Methylococcus capsulatus and Methanomonas methanooxidans. Biochemical Journal 115:631–639
    [Google Scholar]
  10. Lineweaver H., Burk D. 1934; The determination of enzyme dissociation constants. Journal of the American Chemical Society 56:658–666
    [Google Scholar]
  11. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  12. Nash T. 1953; The colorimetric estimation of formaldehyde by means of the Hantzsch reaction. Biochemical Journal 55:416–421
    [Google Scholar]
  13. Ogata K., Tani Y., Kato N. 1975; Oxidation of methanol by yeasts. In Microbial Growth on C1Compounds pp. 99–119 Tokyo: Society of Fermentation Technology, Japan;
    [Google Scholar]
  14. Pfennig N. 1969; Rhodopseudomonas acidophila, sp.n., a new species of the budding purple nonsulfur bacteria. Journal of Bacteriology 99:597–602
    [Google Scholar]
  15. Quayle J. R. 1972; The metabolism of one-carbon compounds by micro-organisms. In Advances in Microbial Physiology 7 pp. 119–203 Rose A. H., Tempest D. W. Edited by London and New York: Academic;
    [Google Scholar]
  16. Quayle J. R., Keech D. B. 1959; Carbon assimilation by Pseudomonas oxalaticus (oxi). 2. Formate and carbon dioxide utilization during growth on formate. Biochemical Journal 72:631–637
    [Google Scholar]
  17. Quayle J. R., Pfennig N. 1975; Utilization of methanol by Rhodospirillaceae. Archives of Microbiology 102:193–198
    [Google Scholar]
  18. Sahm H., Wagner F. 1973; Mikrobielle Verwertung von Methanol. Eigenschaften der Formaldehyde-dehydrogenase und der Formiatdehydrogenase aus Candida boidinii. Archiv für Mikrobiologie 90:263–268
    [Google Scholar]
  19. Salem A. R., Hacking A. J., Quayle J. R. 1973; Cleavage of malyl-coenzyme A into acetyl-coenzyme A and glyoxylate by Pseudomonas ami and other C1-unit-utilizing bacteria. Biochemical Journal 136:89–96
    [Google Scholar]
  20. Salem A. R., Large P. J., Quayle J. R. 1972; Glycine formation during growth of Pseudomonasami on methanol and succinate. Biochemical Journal 128:1203–1211
    [Google Scholar]
  21. Slater J. H., Morris I. 1973; Pathway of carbon dioxide assimilation in Rhodospirillum rubrum grown in turbidostat continuous flow culture. Archiv für Mikrobiologie 92:235–244
    [Google Scholar]
  22. Sperl G. T., Forrest H. S., Gibson D. T. 1974; Substrate specificity of the purified primary alcohol dehydrogenases from methanol-oxidizing bacteria. Journal of Bacteriology 118:541–550
    [Google Scholar]
  23. Stokes J. E., Hoare D. S. 1969; Reductive pentose cycle and formate assimilation in Rhodopseudomonas palustris. Journal of Bacteriology 100:890–894
    [Google Scholar]
  24. Strøm T., Ferenci T., Quayle J. R. 1974; The carbon assimilation pathways of Methylococcus capsulatus, Pseudomonas methanica and Methylosinus trichosporium (0B3B) during growth on methane. Biochemical Journal 144:465–476
    [Google Scholar]
  25. Yoch D. C., Lindstrom E. S. 1967; Photosynthetic conversion of formate and CO2 to glutamate by Rhodopseudomonas palustris. Biochemical and Biophysical Research Communications 28:65–69
    [Google Scholar]
  26. Yoch D. C., Lindstrom E. S. 1969; Nicotinamide adenine dinucleotide-dependent formate dehydrogenase from Rhodopseudomonas palustris. Archiv für Mikrobiologie 67:182–188
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-94-2-313
Loading
/content/journal/micro/10.1099/00221287-94-2-313
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error