Methionine Degradation by UK1 and its Methionine-utilizing Mutant Free

Abstract

Summary: UK1, and a mutant derived from it that can use methionine as its sole source of carbon and nitrogen, were used to study methionine degradation. Radioactive 2-oxomethionine, 2-oxobutyric acid and carbon dioxide comprised 95% of [1-C]methionine consumed by the mutant. Demethio-lating activity was detected in both strains when they were grown with methionine.

The ability to grow on methionine depended on the decarboxylation of 2-oxobutyric acid derived from it. This decarboxylating activity was lost if the growth media contained an additional carbon source.

No evidence was obtained for the operation of the cystathionine pathway.

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1976-06-01
2024-03-29
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