1887

Abstract

Summary: UK1, and a mutant derived from it that can use methionine as its sole source of carbon and nitrogen, were used to study methionine degradation. Radioactive 2-oxomethionine, 2-oxobutyric acid and carbon dioxide comprised 95% of [1-C]methionine consumed by the mutant. Demethio-lating activity was detected in both strains when they were grown with methionine.

The ability to grow on methionine depended on the decarboxylation of 2-oxobutyric acid derived from it. This decarboxylating activity was lost if the growth media contained an additional carbon source.

No evidence was obtained for the operation of the cystathionine pathway.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-94-2-305
1976-06-01
2021-05-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/94/2/mic-94-2-305.html?itemId=/content/journal/micro/10.1099/00221287-94-2-305&mimeType=html&fmt=ahah

References

  1. Ariga N. 1972; Thin-layer chromatography of keto acid 2,4-dinitrophenylhydrazones. Analytical Biochemistry 49:436–441
    [Google Scholar]
  2. Bailey R. B., Parks L. W. 1972; Response of the intracellular adenosine triphosphate pool of Saccharomyces cerevisiae to growth inhibition induced by excess l-methionine. Journal of Bacteriology III:542–546
    [Google Scholar]
  3. Bieleski R. L., Turner N. A. 1966; Separation and estimation of amino acids in crude plant extracts by thin-layer chromatography. Analytical Biochemistry 17:278–293
    [Google Scholar]
  4. Challenger F. 1959 Aspects of the Organic Chemistry of Sulphur. New York: Academic;
    [Google Scholar]
  5. Delavier-Klutcko C., Flavin M. 1965; Enzymatic synthesis and cleavage of cystathionine in fungi and bacteria. Journal of Biological Chemistry 240:2537–2549
    [Google Scholar]
  6. Flavin M. 1962; Microbial transsulfuration: the mechanism of an enzymatic disulfide elimination reaction. Journal of Biological Chemistry 237:768–777
    [Google Scholar]
  7. Goodhue C. T., Snell E. E. 1966; The bacterial degradation of pantothenic acid. I. Overall nature of the reaction. Biochemistry, New York 5:393–398
    [Google Scholar]
  8. Greene R. C. 1957; Source of the three carbon chain of spermidine. Federation Proceedings 16:189
    [Google Scholar]
  9. Jakoby G. A. 1964; The induction and repression of amino acid oxidation in Pseudomonas fluorescens. Biochemical Journal 92:1–8
    [Google Scholar]
  10. Kallio R. E., Larson A. D. 1955; Methionine degradation by a species of Pseudomonas. In A Symposium on Amino Acid Metabolism pp. 616–631 McElroy W. D., Glass H. B. Edited by Baltimore: Johns Hopkins Press;
    [Google Scholar]
  11. Katzuki H., Yoshida T., Tanegashima C., Tanaka S. 1971; Improved direct method for determination of keto acids by 2,4-dinitrophenylhydrazine. Analytical Biochemistry 43:349–356
    [Google Scholar]
  12. Kreis W., Hession C. 1973; Isolation and purification of l-methionine-α-deamino-γ-mercapto- methane-lyase (l-methioninase) from Clostridium sporogenes. Cancer Research 33:1862–1865
    [Google Scholar]
  13. Mäntsälä P., Laakso S., Nurmikko V. 1974; Observations on methionine transport in Pseudomonas fluorescens uki. Journal of General Microbiology 84:19–27
    [Google Scholar]
  14. Meister A. J. 1952; Enzymatic preparation of α-ketoacids. Journal of Biological Chemistry 197:309–317
    [Google Scholar]
  15. Miwatani T., Omukai Y., Nakada D. 1954; Enzymatic cleavage of methionine and homocysteine by bacteria. Medical Journal of Osaka University 5:347–352
    [Google Scholar]
  16. Ruiz-Herrera J., Starkey R. L. 1969; Dissimilation of methionine by fungi. Journal of Bacteriology 99:544–551
    [Google Scholar]
  17. Ruiz-Herrera J., Starkey R. L. 1970; Dissimilation of methionine by Achromobacter starkeyi. Journal of Bacteriology 104:1286–1293
    [Google Scholar]
  18. Segal W., Starkey R. L. 1953; Quantitative determination of methyl mercaptan, dimethyl disulphide and dimethyl sulphide in a gas mixture. Analytical Chemistry 25:1645–1648
    [Google Scholar]
  19. Segal W., Starkey R. L. 1969; Microbial decomposition of methionine and identity of the resulting products. Journal of Bacteriology 98:908–913
    [Google Scholar]
  20. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic Pseudomonas: a taxonomic study. Journal of General Microbiology 43:159–271
    [Google Scholar]
  21. Yall I., Norrel S. A., Joseph R., Knudsen R. C. 1967; Effect of l-methionine and S-adenosyl-methionine on growth of an adenine mutant of Saccharomyces cerevisiae. Journal of Bacteriology 93:1551–1558
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-94-2-305
Loading
/content/journal/micro/10.1099/00221287-94-2-305
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error