1887

Abstract

Summary: The DNA homology relationships of 25 micrococci (15 strains of Micrococcus, eight strains of Sarcina and two strains of Staphylococcus) were studied by the deoxyribonucleic acid hybridization method using nuclease S, an endonuclease specific for single-stranded DNA molecules. Nineteen of the strains were classified into three groups. Group I contained 1056, 11010, 12005 and 12006, 12007 and 12008, 12009, 381 and 382, and 11006. Group II contained 1315, 412, 185 and 1295. Group III contained 1099, 3232 and 383, 399 and 15306. 1097, 19099 and 19100, 1459 and 1470, and 1011 could not be assigned to any of the three groups.

The grouping corresponds to that derived from the results of differential lysis by lysozyme, ‘lytic enzyme 2’ from sp., or G enzyme; and to types of peptidoglycan in the cell walls and genetic transformation. The usefulness of classification based on sensitivity to various lytic enzymes was demonstrated. Group I probably coincides with of (1974), and groups II and III with and respectively.

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1976-05-01
2021-05-18
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