1887

Abstract

Summary: Two phage-bound polysaccharide hydrolases specific for type 8 polysaccharides were isolated. Each enzyme was specific for the polysaccharide produced by the host strain. One enzyme hydrolysed a pyruvylated and acetylated polymer, while the other only acted on the substrate lacking these substituents. Both enzymes were galactosidases releasing tetrasaccharides from their substrates which were only hydrolysed to a limited extent.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-94-1-211
1976-05-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/94/1/mic-94-1-211.html?itemId=/content/journal/micro/10.1099/00221287-94-1-211&mimeType=html&fmt=ahah

References

  1. Adams M. H., Park B. H. 1956; An enzyme produced by a phage-host system. II. The properties of the polysaccharide depolymerase. Virology 2:719–736
    [Google Scholar]
  2. Bessler W., Freund-Moelbert E., Knuefermann C., Rudolph C., Thurow H., Stirm S. 1973; A bacteriophage-induced depolymerase (prolysin 11) active on Klebsiella KII capsular polysaccharide. Virology 56:134–151
    [Google Scholar]
  3. Bitter T., Muir H. M. 1962; A modified uronic acid carbazole reaction. Analytical Biochemistry 4:330–334
    [Google Scholar]
  4. Dudman W. F., Wilkinson J. F. 1956; The composition of the extracellular polysaccharide of Aerobacter-Klebsiella strains. Biochemical Journal 62:289–295
    [Google Scholar]
  5. Eklund C., Wyss O. 1962; Enzyme associated with bacteriophage infection. Journal of Bacteriology 84:1209–1215
    [Google Scholar]
  6. Grant W. M. 1948; Colorimetric micro-determination of formic acid based on reduction to formaldehyde. Analytical Chemistry 20:267–269
    [Google Scholar]
  7. Hestrin S. 1949; The reaction of acetylcholine and other carboxylic acid derivatives with hydroxylamine and its analytical application. Journal of Biological Chemistry 193:249–261
    [Google Scholar]
  8. Kauffmann F., Vahlne G. 1945; The serology of the Klebsiella group. Actapathologica et microbiologicascandinavica 22:119–135
    [Google Scholar]
  9. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  10. Sloneker J. H., Orentas D. G. 1962; Pyruvic acid, a unique component of an exocellular bacterial polysaccharide. Nature; London: 194478–479
    [Google Scholar]
  11. Stirm S. 1968; Escherichia coli K bacteriophages. I. Isolation and introductory characterization of five Escherichia coli K bacteriophages. Journal of Virology 2:1107–1114
    [Google Scholar]
  12. Stirm S., Freund-Moelbert E. 1971; Escherichia coli capsule bacteriophages. II. Morphology. Journal of Virology 8:330–342
    [Google Scholar]
  13. Sutherland I. W. 1970; Structure of Klebsiellaaerogenes type 8 polysaccharide. Biochemistry, New York 9:2180–2185
    [Google Scholar]
  14. Sutherland I. W. 1971; Enzymic hydrolysis of colanic acid. European Journal of Biochemistry 23:582–587
    [Google Scholar]
  15. Sutherland I. W., Wilkinson J. F. 1968; The exopolysaccharide of Klebsiellaaerogenes A3 (S1) (type 54). Biochemical Journal749–754
    [Google Scholar]
  16. Thurow H., Niemann H., Rudolph C., Stirm S. 1974; Host capsule depolymerase activity of bacteriophage particles active on Klebsiella K20 and K24 strains. Virology 58:306–309
    [Google Scholar]
  17. Yurewicz E. C., Ghalambor M. A., Duckworth D. H., Heath E. C. 1971; Catalytic and molecular properties of a phageinduced capsular polysaccharide depolymerase. Journal of Biological Chemistry 246:5607–5616
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-94-1-211
Loading
/content/journal/micro/10.1099/00221287-94-1-211
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error