@article{mbs:/content/journal/micro/10.1099/00221287-94-1-204, author = "Gealt, M.A. and Sheir-Neiss, G. and Morris, N.R.", title = "The Isolation of Nuclei from the Filamentous Fungus Aspergillus nidulans", journal= "Microbiology", year = "1976", volume = "94", number = "1", pages = "204-210", doi = "https://doi.org/10.1099/00221287-94-1-204", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-94-1-204", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Summary: A method has been developed for isolating nuclei from the filamentous fungus Aspergillus nidulans. In this procedure, the mycelia from 14 to 16 h spore-derived cultures of A. nidulans NR1, a stable diploid strain, were frozen with liquid nitrogen and homogenized in a Waring blender. After homogenization, the mycelia were warmed to 4 °C and the nuclei were purified from the homogenate by differential centrifugation followed by sedimentation through 2·1 m-sucrose. The final nuclear yield was 15 to 20%, based on DNA estimations. The purified nuclear pellet was free of whole cells. The morphology of the isolated nuclei resembled that of the in situ nuclei; they contained a nucleolus, chromatin, and had a surrounding double membrane. The purified nuclei were characterized by a DNA:RNA: protein ratio of 1:2.8:8·7.", }