1887

Abstract

Summary: An alkali-soluble glucan was obtained from the cell walls of NCYC1109 and baker's yeast by extraction with cold, dilute sodium hydroxide under nitrogen. The glucan, which represented approximately 20% of the cell wall, precipitated as a gel when the alkaline extract was neutralized. The purified glucan was homogeneous and was shown to be free from contamination by other cell-wall polysaccharides by ultracentrifuging, gel filtration and electro-phoresis. In addition to glucose, the glucan contained traces of mannose and nitrogen, but no hexosamine. Structural analyses revealed the presence of 80–85% (1→3)-β- linkages, 8–12% (1→6)-β- linkages and 3·4% branched residues linked through C-1, C-3 and C-6. The molecular weight of the glucan was estimated to be about 250000. Electron-microscopic examination of the cell walls after alkali extraction showed that an amorphous surface layer had been removed revealing numerous bud scar structures.

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1976-05-01
2021-05-11
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References

  1. Aspinall G. O., Ferrier R. J. 1957; A spectrophotometric method for the determination of periodate consumed during the oxidation of carbohydrates. Chemistry and Industry1216
    [Google Scholar]
  2. Bacon J. S. D., Jones D., Farmer V. C., Webley D. M. 1968; The occurrence of α-(1→3)-glucan in Cryptococcus, Schizosaccharomyces and Polyporus species, and its hydrolysis by a Streptomyces culture filtrate lysing cell walls of Cryptococcus. Biochimica et biophysica acta 158:313–315
    [Google Scholar]
  3. Bacon J. S. D., Farmer V. C., Jones D., Taylor I. F. 1969; The glucan components of the cell wall of baker’s yeast (Saccharomyces cerevisiae) considered in relation to its ultrastructure. Biochemical Journal 114:557–567
    [Google Scholar]
  4. Ballou C. E. 1974; Some aspects of the structure, biosynthesis and genetic control of yeast mannans. . In Advances in Enzymology 40 pp. 239–270 Meister A. Edited by New York: Wiley;
    [Google Scholar]
  5. Bjorndal H., Hellerqvist C. G., Lindberg B., Svenson S. 1970; Gas-liquid chromatography and mass spectrometry in methylation analysis of polysaccharides. Angewandte Chemie International Edition in English 9:610–619
    [Google Scholar]
  6. Bowden J. K., Hodgson B. 1970; Evidence against the presence of fibres or chemically distinct layers in the cell wall of Saccharomyces. Antonie van Leeuwenhoek 36:81–108
    [Google Scholar]
  7. Bush O. A., Horisberger M., Horman I., Wursch P. 1974; The wall structure of Schizosaccharomyces pombe. Journal of General Microbiology 81:199–206
    [Google Scholar]
  8. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  9. Eddy A. A., Woodhead J. S. 1968; An alkali-soluble glucan fraction from the cell walls of the yeast Saccharomyces carlsbergensis. Federation of European Biochemical Societies Letters 1:67–68
    [Google Scholar]
  10. Elson L. A., Morgan W. T. J. 1933; A colorimetric method for the determination of glucosamine and chondrosamine. Biochemical Journal 27:1824–1828
    [Google Scholar]
  11. Fleet G. H., Manners D. J. 1975; Gel chromatography of polysaccharides. Transactions of the Biochemical Society 3:981–983
    [Google Scholar]
  12. Fleet G. H., Phaff H. J. 1973; Effect of glucanases of yeast and bacterial origin on cell walls of Schizosaccharomyces species. . In Yeast, Mould and Plant Protoplasts pp. 33–59 Villanueva J. R., Garcia-Acha I., Gascón S., Urubaru F. Edited by London: Academic;
    [Google Scholar]
  13. Fleet G. H., Phaff H. J. 1974a; Lysis of yeast cell walls: Glucanases from Bacillus circulans WL-12. Journal of Bacteriology 119:207–219
    [Google Scholar]
  14. Fleet G. H., Phaff H. J. 1974b; Glucanases in Schizosaccharomyces: isolation and properties of the cell wall-associated β-(1→3)-glucanases. Journal of Biological Chemistry 249:1717–1728
    [Google Scholar]
  15. Fleming M., Hirst E., Manners D. J. 1966; The constitution of laminarin. Part VI. The fine structure of soluble laminarins. In Proceedings of the Fifth International Seaweed Symposium pp. 255–260 Oxford and New York: Pergamon;
    [Google Scholar]
  16. Goldstein I. J., Hay G. W., Lewis B. A., Smith F. 1965; Controlled degradation of polysaccharides by periodate oxidation, reduction and hydrolysis. In Methods in Carbohydrate Chemistry V pp. 361–370 Whistler R. L. New York and London: Academic;
    [Google Scholar]
  17. Gunja-Smith Z., Smith E. 1974; Evidence for the periplasmic location of glycogen in Saccharomyces. Biochemical and Biophysical Research Communications 56:588–592
    [Google Scholar]
  18. Hellerqvist C. G., Lindberg B., Samuelsson K. 1968; Methylation analysis of pustulan. Acta chemica scandanavica 22:2736–2737
    [Google Scholar]
  19. Hoffmann G. C., Simson B. N., Timell R. E. 1971; Structure and molecular size of pachyman. Carbohydrate Research 20:185–188
    [Google Scholar]
  20. Houwink A. L., Kreger D. R. 1953; Observations on the cell wall of yeast. Antonie van Leeuwenhoek 19:1–24
    [Google Scholar]
  21. Huggett A. ST. G., Nixon D. A. 1957; Enzymic determination of blood glucose. Biochemical Journal 66:12P
    [Google Scholar]
  22. Jones D., Gordon A. H., Bacon J. S. D. 1974; Co-operative action by endo- and exo-β-(1→3)-glu- canases from parasitic fungi in the degradation of cell wall glucans of Sclerotinia sclerotium (Lib) de Bary. Biochemical Journal 140:47–55
    [Google Scholar]
  23. Kessler G., Nickerson W. J. 1959; Glucomannan-protein complexes from cell walls of yeasts. Journal of Biological Chemistry 234:2281–2283
    [Google Scholar]
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