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Volume 94, Issue 1

Decreased Permeability as a Mechanism of Resistance to Methyl Benzimidazol-2-yl Carbamate (MBC) in Free

Abstract

Summary: Mutants of resistant to benzimidazole fungicides varied in their responses to 2-(thiazol-4-yl)benzimidazole (thiabendazole, TBZ), methyl I-(butylcarbamoyl)-benzimidazol-2-yl carbamate (benomyl) and methyl benzimidazol-2-yl carbamate (carbendazim, MBC). Incorporation of [C]MBC into trichloroacetic acid extracts of the sensitive strain S increased during a 2 h incubation period, whereas incorporation into the resistant mutant M55 was unchanged. [C]MBC uptake by S4 cells was five times higher than that by M55. MBC was identified as the main radioactive compound inside the S4 cells and reached a level of 2·4 g/100 mg dry wt.

The compound MBC enters the cells of by a temperature-, energy-, pH- and concentration-dependent transport system which may be specific for compounds containing a benzimidazole nucleus. It is suggested that tolerance of 55 to MBC is due to decreased permeability of the cell to this compound

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© Society for General Microbiology 1976
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1976-05-01
2025-01-21
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References

  1. Aharonson N., Kafkafi U. 1975; Adsorption of benzimidazole fungicides on montmorillonite and kaolinite clay surface. Journal of Agricultural and Food Chemistry 23:434–437
     [Google Scholar]
  2. Bollen G. J. 1971; Resistance to benomyl in strains of Penicillium sp. Netherlands Journal of Plant Pathology 77:187–192
     [Google Scholar]
  3. Byrde R. J. W. 1970; The new systemic fungicides and their potential use in the tropics. Tropical Science 12:105–108
     [Google Scholar]
  4. Clemons G. P., Sisler H. D. 1969; Formation of a fungitoxic derivative from benlate. Phytopathology 59:705–708
     [Google Scholar]
  5. Clemons G. P., Sisler H. D. 1971; Localization of the site of action of a fungitoxic benomyl derivative. Pesticide Biochemistry and Physiology 1:32–43
     [Google Scholar]
  6. Davidse L. C. 1973; Antimitotic activity of methyl benzimidazole-2-yl carbamate (MBC) in Aspergillus nidulans.. Pesticide Biochemistry and Physiology 3:317–329
     [Google Scholar]
  7. Erwin D. C. 1973; Systemic fungicides: disease control, translocation and mode of action. Annual Review of Phytopathology 11:389–422
     [Google Scholar]
  8. Evans E. 1971; Systemic fungicides in practice. Pesticide Science 2:192–196
     [Google Scholar]
  9. Gross Y., Kenneth R. 1973; The fate of benomyl and thiabendazole in seed and soil treated plants. Annals of Applied Biology 73:307–318
     [Google Scholar]
  10. Hammerschlag R. S., Sisler H. D. 1972; Differential action of benomyl and methyl-2-benzimidazole carbamate (MBC) in Saccharomyces pastorianus.. Pesticide Biochemistry and Physiology 2:126–131
     [Google Scholar]
  11. Lindegren G., Hwang Y. L., Oshima Y. G., Lindegren C. L. 1965; Genetical mutants induced by ethyl methanesulphonate in Saccharomyces. Canadian Journal of Genetics and Cytology 7:491–499
     [Google Scholar]
  12. Schroeder W. T., Prowidenti R. 1969; Resistance to benomyl in powdery mildew of cucurbits. Plant Disease Reporter 53:271–273
     [Google Scholar]
  13. Stutzenberger F. J., Parle J. N. 1972; Binding of benzimidazole compounds to conidia of Pit homy ces chartarum.. Journal of General Microbiology 73:85–94
     [Google Scholar]
/content/journal/micro/10.1099/00221287-94-1-167
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Decreased Permeability as a Mechanism of Resistance to Methyl Benzimidazol-2-yl Carbamate (MBC) in Sporobolomyces roseus
Microbiology 94, 167 (1976); https://doi.org/10.1099/00221287-94-1-167
/content/journal/micro/10.1099/00221287-94-1-167
/content/journal/micro/10.1099/00221287-94-1-167
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