1887

Abstract

Summary: An unusual 12 mutant for carbamyl phosphate synthetase is described. The mutation was generated by bacteriophage Mu1 insertion and left a 5 % residual activity of the enzyme using either ammonia or glutamine as donors. The mutation is recessive to the wild-type allele and maps at or near the gene, but the mutant requires only arginine and not uracil for growth. By a second block in the gene it was possible to shift the accumulated carbamyl phosphate to arginine biosynthesis. The values and the levels of ornithine activation and inhibition by UMP were normal in the mutant enzyme.

It was possible to distinguish serologically between and and the - group. The absence of consistent serological variation between and is consistent with the suggestion (Bumbieris, 1974) that and should be considered as one species.

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/content/journal/micro/10.1099/00221287-94-1-142
1976-05-01
2021-05-15
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