1887

Abstract

Summary: Spores of were treated with hydrogen peroxide until their peripheries had lost refractility. The centres of such spores only retained refractility at acid pH. Adding monovalent cations or increasing the pH caused the treated spores to lose their remaining refractility and decreased the turbidity of spore suspensions. Divalent cations prevented or reversed this loss of central refractility and decreased the fall in turbidity. Calcium ions also prevented but did not reverse the loss of central refractility which occurred on drying or applying pressure. Electron micrographs of spores treated with hydrogen peroxide showed that the cortex was depleted or absent and that the loss of central refractility was accompanied by protoplast swelling. It is suggested that divalent cations make spores resistant to drying and pressure by cross-linking negatively charged groups within the protoplast, and that together with hydrogen ions they neutralize the negatively charged groups, thus preventing the swelling of the protoplast, loss of refractility and fall in extinction which occur when divalent cations are replaced by monovalent cations.

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1976-04-01
2024-11-04
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References

  1. Dring G.J., Gould G.W. 1971; Movement of potassium during l-alanine-initiated germination of Bacillus subtilis spores. In Spore Research- 1971 pp. 133–141 Barker A. N., Gould G. W., Wolf J. Edited by London: Academic Press;
    [Google Scholar]
  2. Edebo L. 1961; Lysis of bacteria. III. On the stability of protoplasts and spheroplasts in different pH ranges. Acta pathologica et microbiologica scandinavica 53:121–128
    [Google Scholar]
  3. Ehrström M., Eriksson L.E.G., Israelachvili J., Ehrenberg A. 1973; The effects of some cations and anions on spin labelled cytoplasmic membranes of Bacillus subtilis. Biochemical and Biophysical Research Communications 55:396–402
    [Google Scholar]
  4. Ellar D.J., Eaton M.W., Posgate J. 1974; Calcium release and germination of bacterial spores. Biochemical Society Transactions 2:947–948
    [Google Scholar]
  5. Fitz-James P.C. 1971; Formation of protoplasts from resting spores. Journal of Bacteriology 205:1119–1136
    [Google Scholar]
  6. Gould G.W., Dring G.J. 1972; Biochemical mechanisms of spore germination. In Spores V pp. 401–408 Halvorson H. O., Hanson R., Campbell L. L. Edited by Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  7. Grula E.A., Hartsell S.E. 1957; Lysozyme in the bacteriolysis of Gram-negative bacteria. II. Factors influencing clearing during the Nakamura treatment. Canadian Journal of Microbiology 3:23–34
    [Google Scholar]
  8. Hashimoto T., Frieben W.R., Conti S.F. 1969; Microgermination of Bacillus cereusspores. Journal of Bacteriology 100:1385–1392
    [Google Scholar]
  9. Haworth F., Cleaver T.J. 1961; Flame-photometric determination of calcium and magnesium in vegetables. Journal of the Science of Food and Agriculture 12:848–852
    [Google Scholar]
  10. Hirsch A., Grinsted E. 1954; Methods for the growth and enumeration of anaerobic spore-formers from cheese, with observations on the effect of nisin. Journal of Dairy Research 21:101–110
    [Google Scholar]
  11. Hitchins A.D., Gould G.W. 1964; Release of cores from bacterial spores by mechanical breakage in acidic media. Nature; London: 203895–896
    [Google Scholar]
  12. Janssen F.W., Lund A.J., Anderson L.E. 1958; Colorimetric assay for dipicolinic acid in bacterial spores. Science; New York: 12726–27
    [Google Scholar]
  13. Joos R.W., Carr C.W. 1967; The binding of calcium in mixtures of phospholipids. Proceedings of the Society for Experimental Biology and Medicine 124:1268–1272
    [Google Scholar]
  14. Kawata T., Inoue T. 1963; Spheroidal and polyhedral forms of Clostridium butyricum induced by penicillin. Journal of Bacteriology 86:168–170
    [Google Scholar]
  15. Lewis J.C., Snell N.L., Burr H.K. 1960; Water permeability of bacterial spores and the concept of a contractile cortex. Science; New York: 132544–545
    [Google Scholar]
  16. Linehan D.J. 1966; The effects of various intrinsic factors on the texture of the cooked potato as affected by environmental conditions. Ph.D. Thesis University of East Anglia:
    [Google Scholar]
  17. Murrell W.G. 1969; Chemical composition of spores and spore structures. In The Bacterial Spore pp. 215–273 Gould G. W., Hurst. A. Edited by London: Academic Press;
    [Google Scholar]
  18. Ogata S., Hongo M. 1973; Bacterial lysis of Clostridium species. I. Lysis of Clostridium species by univalent cation. Journal of General and Applied Microbiology 19:251–261
    [Google Scholar]
  19. Papahadjopoulos D., Ohki S. 1969; Stability of asymmetric phospholipid membranes. Science; New York: 1641075–1077
    [Google Scholar]
  20. Pearce S.M., Fitz-James P.C. 1971; Sporulation of a cortexless mutant of a variant of Bacillus cereus. Journal of Bacteriology 105:339–348
    [Google Scholar]
  21. Powell J.F., Strange R.E. 1953; Biochemical changes occurring during the germination of bacterial spores. Biochemical Journal 54:205–209
    [Google Scholar]
  22. Rojas E., Tobias J.M. 1965; Membrane model: association of inorganic cations with phospholipid monolayers. Biochimica et biophysica acta 94:394–404
    [Google Scholar]
  23. Rondle C.J.M., Morgan W.T.J. 1955; The determination of glucosamine and galactosamine. Biochemical Journal 61:586–589
    [Google Scholar]
  24. Sacks L.E. 1972; Influence of intra- and extracellular cations on the germination of bacterial spores. In Spores V pp. 437–442 Halvorson H. O., Hanson R., Campbell. L. L. Edited by Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  25. Slepecky R.A., Foster J.W. 1959; Alteration in metal content of spores of Bacillus megaterium and the effect on some spore properties. Journal of Bacteriology 78:117–123
    [Google Scholar]
  26. Vary J.C. 1973; Germination of Bacillus megaterium spores after various extraction procedures. Journal of Bacteriology 116:798–802
    [Google Scholar]
  27. Vinter V., Šťastná J., Čáslavská J. 1969; Interference of some cations and basic compounds with the germination and outgrowth of bacterial spores. In Spores IV pp. 289–297 Campbell L. L., Bethesda. Edited by Maryland: American Society for Microbiology;
    [Google Scholar]
  28. Waites W.M., Wyatt L.R. 1971; Germination of spores of Clostridium bifermentans by certain amino acids, lactate and pyruvate in the presence of sodium or potassium ions. Journal of General Microbiology 67:215–222
    [Google Scholar]
  29. Waites W.M., Wyatt L.R., Arthur B. 1972; Effect of alkali treatment on the germination and morphology of spores of Clostridium bifermentans. In Spores V pp. 430–436 Halvorson H. O., Hanson R., Campbell L. L. Edited by Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  30. Warth A.D., Ohye D.F., Murrell W.G. 1963a; The composition and structure of bacterial spores. Journal of Cell Biology 16:579–592
    [Google Scholar]
  31. Warth A.D., Ohye D.F., Murrell W.G. 1963b; Location and composition of spore mucopeptide in Bacillusspecies. Journal of Cell Biology 16:593–609
    [Google Scholar]
  32. Wyatt L.R., Waites W.M. 1971; Studies with spores of Clostridium bifermentans-,comparison of germination mutants. In Spore Research- 1971 pp. 123–131 Barker A. N., Gould G. W., Wolf J. Edited by London: Academic Press;
    [Google Scholar]
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