1887

Abstract

Summary: In , the synthesis of histidase, urocanase and amidase is severely repressed when succinate is added to a culture growing in pyruvate + ammonium salts medium. When growth is nitrogen-limited, catabolite repression by succinate of histidase and urocanase synthesis does not occur but succinate repression of amidase synthesis persists. Amidase synthesis is not regulated in the same way as histidase synthesis by the availability of other nitrogen compounds for growth.

Growth of strain PACI in succinate + histidine media is nitrogen-limited since this strain is defective in a histidine transport system. When methyl-ammonium chloride is added to succinate + histidine media, growth inhibition occurs. Mutants isolated from succinate + histidine + methylammonium chloride plates were found to be resistant to catabolite repression by succinate even in ammonium salts media. It is suggested that the genes of may be regulated in the same way as in , by induction by urocanate and activation by either the cyclic AMP-dependent activator protein or by glutamine synthetase.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-93-2-377
1976-04-01
2024-12-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/93/2/mic-93-2-377.html?itemId=/content/journal/micro/10.1099/00221287-93-2-377&mimeType=html&fmt=ahah

References

  1. Arst H.N. 1973; Nitrogen metabolite repression in Aspergillus nidulans.. Molecular and General Genetics 126:111–141
    [Google Scholar]
  2. Brammar W.J., Clarke P.H. 1964; Induction and repression of Pseudomonas aeruginosa amidase. Journal of General Microbiology 37:307–319
    [Google Scholar]
  3. Brammar W.J., Clarke P.H. 1967; Biochemical and genetic studies with regulator mutants of the Pseudomonas aeruginosa 8602 amidase system. Journal of General Microbiology 47:87–102
    [Google Scholar]
  4. Brill W.J., Magasanik B. 1969; Genetic and metabolic control of histidase and urocanase in Salmonella typhimurium strain 15-59 . Journal of Biological Chemistry 244:5392–5402
    [Google Scholar]
  5. De Crombrugghe B., Chen B., Anderson W., Nissley P., Gottesman M., Pastan I., Perlman R. 1971; Lac DNA, RNA polymerase and cyclic AMP receptor protein, cyclic AMP, lac repressor and inducer are the essential elements for controlled lac transcription. Nature New Biology 231:139–142
    [Google Scholar]
  6. Hynes M.J. 1970; Induction and repression of amidase enzymes in Aspergillus nidulans. Journal of Bacteriology 103:482–487
    [Google Scholar]
  7. Hynes M.J. 1972; Mutants with altered glucose repression of amidase enzymes in Aspergillus nidulans. Journal of Bacteriology 111:717–722
    [Google Scholar]
  8. Hynes M.J. 1974; Effect of ammonium, l-glutamate and l-glutamine on nitrogen catabolism in Aspergillus nidulans. Journal of Bacteriology 120:1116–1123
    [Google Scholar]
  9. Kelly M. 1961; An investigation of the action of Pseudomonas aeruginosa on amides and related compounds. Ph.D. Thesis University of London:
    [Google Scholar]
  10. Kelly M., Clarke P.H. 1962; An inducible amidase produced by a strain of Pseudomonas aeruginosa. Journal of General Microbiology 27:305–316
    [Google Scholar]
  11. Lessie T.G., Neidhardt F.C. 1967; Formation and operation of the histidine-degrading pathway in Pseudomonas aeruginosa. Journal of Bacteriology 93:1800–1810
    [Google Scholar]
  12. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. 1951; Protein measurements with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  13. Magasanik B., Prival M.J., Brenchley J.E., Tyler B.M., Deleo A.B., Streicher S.L., Bender R.A., Paris C.G. 1974; Glutamine synthesis as a regulator of enzyme synthesis. Current Topics in Cellular Regulation 8:119–138
    [Google Scholar]
  14. Potts J.R. 1975; Histidine catabolism in Pseudomonas aeruginosa. Ph.D. Thesis University of London;
    [Google Scholar]
  15. Potts J.R., Clarke P.H. 1974; The regulation of histidine catabolism in Pseudomonas aeruginosa. Society for General Microbiology Proceedings 1:63
    [Google Scholar]
  16. Prival M.J., Brenchley J.E., Magasanik B. 1973; Glutamine synthetase and the regulation of histidase formation in Klebsiella aerogenes. Journal of Biological Chemistry 248:4334–4344
    [Google Scholar]
  17. Prival M.J., Magasanik B. 1971; Resistance to catabolite repression of histidase and proline oxidase during nitrogen-limited growth. Journal of Biological Chemistry 246:6288–6296
    [Google Scholar]
  18. Smyth P. F. 1974; Catabolite repression in Pseudomonas aeruginosa. Ph.D. Thesis University of London:
    [Google Scholar]
  19. Smyth P.F., Clarke P.H. 1975a; Catabolite repression of Pseudomonas aeruginosa amidase: The effect of carbon sources on amidase synthesis. Journal of General Microbiology 90:81–90
    [Google Scholar]
  20. Smyth P.F., Clarke P.H. 1975b; Catabolite repression in Pseudomonas aeruginosa amidase: Isolation of promotor mutants. Journal of General Microbiology 90:91–99
    [Google Scholar]
  21. Tronick S.R., Ciardi J.E., Stadtman E.R. 1973; Comparative biochemical and immunological studies of bacterial glutamine synthetases. Journal of Bacteriology 115:858–868
    [Google Scholar]
  22. Tyler B., Deleo A.B., Magasanik B. 1974; Activation of hut DNA by glutamine synthetase. Proceedings of the National Academy of Sciences of the United States of America 71:225–229
    [Google Scholar]
/content/journal/micro/10.1099/00221287-93-2-377
Loading
/content/journal/micro/10.1099/00221287-93-2-377
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error