1887

Abstract

SUMMARY: Oxygen-limited (N-fixing) chemostat cultures of supplied with a N-free medium were established by introducing low atmospheric O concentrations into the gas supply of anaerobic glucose-limited N-fixing chemostat cultures; the molar growth yield for glucose and the efficiency of N fixation (g N fixed/mg glucose consumed) were increased (by up to 82%) from the anaerobic values.

Acetylene-reducing activity was inhibited reversibly by O in samples from O-limited and anaerobic glucose-limited chemostat cultures. Oxygen uptake rates in samples from these chemostat cultures were similar, but CH-reducing activity in samples from O-limited chemostat cultures was more tolerant of low atmospheric O concentrations, in part because of a higher population density. In the absence of glucose, O was required at a low atmospheric concentration for CH reduction in samples from either O-limited or anaerobic glucose-limited chemostat cultures. The possibility is discussed that ATP generated from oxidative phosphorylation can be used for N fixation in

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-93-2-335
1976-04-01
2024-12-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/93/2/mic-93-2-335.html?itemId=/content/journal/micro/10.1099/00221287-93-2-335&mimeType=html&fmt=ahah

References

  1. Baker K. 1968; Low cost continuous culture apparatus. Laboratory Practice 17:817–824
    [Google Scholar]
  2. Biggins D.R., Postgate J.R. 1971; Nitrogen fixation by extracts ofMycobacterium flavum 301, use of natural electron donors and oxygen sensitivity of cell-free preparations. European Journal of Biochemistry 19:408–415
    [Google Scholar]
  3. Chaney A.L., Marbach E.P. 1962; Modified reagents for determination of urea and ammonia. Clinical Chemistry 8:130–132
    [Google Scholar]
  4. Dalton H., Postgate J.R. 1969; Effect of oxygen on growth ofAzotobacter chroococcum in batch and continuous culture. Journal of General Microbiology 56:307–319
    [Google Scholar]
  5. Dixon R.A. 1972 Genetics of nitrogen fixation in coliform bacteria. D.Phil. thesis.; University of Sussex.:
    [Google Scholar]
  6. Drozd J., Postgate J.R. 1970; Effects of oxygen on acetylene reduction, cytochrome content and respiratory activity ofAzotobacter chroococcum. Journal of General Microbiology 63:63–73
    [Google Scholar]
  7. Eady R.R., Postgate J.R. 1974; Nitrogenase. Nature; London: 249805–810
    [Google Scholar]
  8. Harrison D.E.F. 1972; A study of the effect of growth conditions on chemostat-grownKlebsiella aerogenes and kinetic changes of a 500 nm absorption band. Biochimica et biophysica acta 275:83–92
    [Google Scholar]
  9. Harrison D.E.F., Loveless J.E. 1971; The effect of growth conditions on respiratory activity and growth efficiency in facultative anaerobes grown in chemostat culture. Journal of General Microbiology 68:35–43
    [Google Scholar]
  10. Harrison D.E.F., Pirt S.J. 1967; The influence of dissolved oxygen concentration on the respiration and glucose metabolism ofKlebsiella aerogenes during growth. Journal of General Microbiology 46:193–211
    [Google Scholar]
  11. Hill S. 1975; Acetylene reduction byKlebsiella pneumoniae in air related to colony dimorphism on low fixed nitrogen. Journal of General Microbiology 91:207–209
    [Google Scholar]
  12. Hill S., Drozd J.W., Postgate J.R. 1972; Environmental effects on the growth of nitrogen-fixing bacteria. Journal of Applied Chemistry and Biotechnology 22:541–558
    [Google Scholar]
  13. Hill S., Postgate J.R. 1969; Failure of putative nitrogen-fixing bacteria to fix nitrogen. Journal of General Microbiology 58:277–285
    [Google Scholar]
  14. Johnson M.J., Borkowski J., Engblom C. 1964; Steam sterilizable probes for dissolved oxygen measurement. Biotechnology and Bioengineering 6:457–468
    [Google Scholar]
  15. Kelly M. 1969; Comparisons and cross-reactions of nitrogenase fromKlebsiella pneumoniae, Azotobacter chroococcum andBacillus polymyxa. Biochimica et biophysica acta 191:527–540
    [Google Scholar]
  16. Klucas R. 1972; Nitrogen fixation byKlebsiella grown in the presence of oxygen. Canadian Journal of Microbiology 18:1845–1850
    [Google Scholar]
  17. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  18. Möller M.K., Ottolenghi P. 1966; The oxidation of o-dianisidine by H2O2and peroxidase at neutral pH. Comptes rendus des travaux du Laboratoire Carlsberg 35:369–389
    [Google Scholar]
  19. Peck H.D.Jr Gest H. 1957; Formic dehydrogenase and hydrogenylase enzyme complex in coli-aerogenes bacteria. Journal of Bacteriology 73:706–721
    [Google Scholar]
  20. Pirt S.J. 1957; The oxygen requirement of growing cultures of anAerobacter species determined by means of the continuous culture technique. Journal of General Microbiology 16:59–75
    [Google Scholar]
  21. Rliiz-Herrera J., Alvarez A. 1972; A physiological study of formate dehydrogenase, formate oxidase and hydrogenylase fromEscherichia coli k-12. Antonie van Leeuwenhoek 38:479–491
    [Google Scholar]
  22. St.John R.T., Shah V.K., Brill W.J. 1974; Regulation of nitrogenase synthesis by oxygen inKlebsiella pneumoniae. Journal of Bacteriology 119:266–269
    [Google Scholar]
  23. Tubb R.S., Postgate J.R. 1973; Control of nitrogenase synthesis inKlebsiella pneumoniae. Journal of General Microbiology 79:103–117
    [Google Scholar]
  24. Wimpenny J.W.T. 1969; Oxygen and carbon dioxide as regulators of microbial growth and metabolism. Symposium of the Society for General Microbiology 19:161–197
    [Google Scholar]
  25. Yates M.G. 1970; Control of respiration and nitrogen fixation by oxygen and adenine nucleotides in N2-grownAzotobacter chroococcum. Journal of General Microbiology 60:393–401
    [Google Scholar]
  26. Yates M.G., Jones C.W. 1974; Respiration and nitrogen fixation in Azotobacter. Advances in Microbial Physiology 11:97–135
    [Google Scholar]
  27. Yoch D.C. 1974; Electron transport carriers involved in nitrogen fixation by the coliform Klebsiella pneumoniae. Journal of General Microbiology 83:153–164
    [Google Scholar]
/content/journal/micro/10.1099/00221287-93-2-335
Loading
/content/journal/micro/10.1099/00221287-93-2-335
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error