1887

Abstract

SUMMARY: Changes in the ethanol-soluble carbohydrate content of mycelium and sporophores grown on semi-defined media and commercial compost were studied. The accumulation of mannitol in the sporophore during its growth was not accompanied by an increase in mycelial mannitol. The other major soluble carbohydrate of the sporophore, trehalose, decreased throughout the growth of the sporophore; a parallel decrease was observed in the mycelium. The main accumulation of mannitol was in the pileus and stipe of the sporophore and was accompanied by a decrease in the soluble protein content of these tissues. Before fruiting, glucose and sucrose were present in the mycelial samples in similar quantities to mannitol, but their levels decreased during fruiting. Small quantities of glucose were present in the sporophore. The results are discussed in relation to the possible functions of the soluble carbohydrates.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-93-2-309
1976-04-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/93/2/mic-93-2-309.html?itemId=/content/journal/micro/10.1099/00221287-93-2-309&mimeType=html&fmt=ahah

References

  1. Bonner J.T, Kent Kane K., Levey R.H. 1956; Studies on the mechanics of growth in the common mushroomAgaricus campestris. Mycologia 48:13–19
    [Google Scholar]
  2. Cochrane V.W. 1958; Carbon metabolism. II. The metabolism of carbohydrates and related compounds. In Physiology of the Fungi pp. 108–122 New York: John Wiley;
    [Google Scholar]
  3. Delmas J. 1973; Valeur alimentaire du champignon de couche Agaricus bisporus cultive. Bulletin de la Federation Nationale des Syndicats Agricoles des Cultivateurs de Champignons 13:537–557
    [Google Scholar]
  4. Diem K., Lentner C. 1970 In Geigy Scientific Tables p. 504 Macclesfield: Geigy Pharmaceuticals;
    [Google Scholar]
  5. Dütsch G.A., Rast D. 1972; Biochemische Beziehung zwischen Mannitbildung und Hexosemono-phosphatzyklus in Agaricus bisporus. Phytochemistry II:2677–2681
    [Google Scholar]
  6. Edmundowicz J.M., Wriston J.C.Jr 1963; Mannitol dehydrogenase from Agaricus campestris. Journal of Biological Chemistry 238:3539–3541
    [Google Scholar]
  7. Graham D.G. 1971 Protein and energy metabolism of mitochondria of the common meadow mushroom (Agaricus bisporus) in extracellular culture. Ph.D, thesis Duke University, U.S.A.:
    [Google Scholar]
  8. Hammond J.B.W., Nichols R. 1975; Changes in respiration and soluble carbohydrates during the post-harvest storage of mushrooms (Agaricus bisporus). Journal of the Science of Food and Agriculture 26:835–842
    [Google Scholar]
  9. Holtz R.B. 1971; Qualitative and quantitative analyses of free neutral carbohydrates in mushroom tissue by gas-liquid chromatography and mass spectrometry. Journal of Agricultural and Food Chemistry 19:1272–1273
    [Google Scholar]
  10. Hughes H.D., Lynch D.L., Somers G.F. 1958; Chromatographic identification of the amino acids and carbohydrates in the cultivated mushroom, Agaricus campestris L. ex Fries. Journal of Agricultural and Food Chemistry 6:850–853
    [Google Scholar]
  11. Le Roux P. 1967; Métabolisme carboné et azoté du champignon cultivé. Mushroom Science 6:179–190
    [Google Scholar]
  12. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  13. Madelin M.F. 1960; Visible changes in the vegetative mycelium ofCoprinus lagopus Fr. at the time of fruiting. Transactions of the British Mycological Society 43:105–110
    [Google Scholar]
  14. McConnell J.E.W., Esselen W.B. 1947; Carbohydrates in cultivated mushrooms (Agaricus campestris). Food Research 12:118–121
    [Google Scholar]
  15. Rast D. 1965; Zur stoffwechselphysiologischen Bedeutung von Mannit und Trehalose in Agaricus bisporus (eine gaschromatographische Studie). Planta 64:81–93
    [Google Scholar]
  16. Schwalb M.N. 1974; Changes in activity of enzymes metabolising glucose-6-phosphate during development of the Basidiomycete Schizophyllum commune. Developmental Biology 40:84–89
    [Google Scholar]
  17. Smith J.F., Hayes W.A. 1972; Use of autoclaved substrates in nutritional investigations on the cultivated mushroom. Mushroom Science 8:355–361
    [Google Scholar]
  18. Sweeley C.C., Bentley R., Makita M., Wells W.W. 1963; Gas-liquid chromatography of tri-methylsilyl derivatives of sugars and related substances. Journal of the American Chemical Soceity 85:2497–2507
    [Google Scholar]
  19. Vogel F.S., Weaver R.F. 1972; Concerning the induction of dormancy in spores of Agaricus bisporus. Experimental Cell Research 75:95–104
    [Google Scholar]
  20. Wessels J.G.H. 1965; Morphogenesis and biochemical processes inSchizophyllum commune Fr. Wentia 13:1–113
    [Google Scholar]
  21. Wong A.-L., Willetts H.J. 1974; Polyacrylamide-gel electrophoresis of enzymes during morphogenesis of sclerotia of Sclerotinia sclerotiorum. Journal of General Microbiology 81:101–109
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-93-2-309
Loading
/content/journal/micro/10.1099/00221287-93-2-309
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error