1887

Abstract

SUMMARY: Human polymorphonuclear leucocytes treated with leucocidin from underwent characteristic morphological alterations as shown by phase-contrast and scanning electron microscopy. Within a few minutes of exposure to leucocidin the granulocytes became round, and protoplasmic extrusions appeared on the cell membrane, were withdrawn again and put out at another point of the cell. The final stage of the leucocidin-treated leucocyte was an enlarged, rounded vesicle with apparently intact plasma membrane. Omission of calcium ions from the diluting buffer caused certain differences in the morphologic appearance of the damaged leucocytes.

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/content/journal/micro/10.1099/00221287-93-2-303
1976-04-01
2021-05-18
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References

  1. Bernheimer A.W. 1947; Comparative kinetics of hemolysis induced by bacterial and other hemolysins. Journal of General Physiology 30:337–353
    [Google Scholar]
  2. Davson H., Danielli J.F. 1938; Studies on the permeability of erythrocytes. V. Factors in cation permeability. Biochemical Journal 32:991–1001
    [Google Scholar]
  3. Dulbecco R., Vogt M. 1954; One-step growth curve of western equine encephalomyelitis virus chicken embryo cells grownin vitro and analysis of virus yields from single cells. Journal of Experimental Medicine 99:183–199
    [Google Scholar]
  4. Gladstone G.P., Van Heyningen W.E. 1957; Staphylococcal leucocidins. British Journal of Experimental Pathology 38:123–137
    [Google Scholar]
  5. Goldberg B., Green H. 1959; The cytotoxic action of immune gamma globulin and complement on Krebs ascites tumor cells. I. Ultrastructural studies. Journal of Experimental Medicine 109:505–510
    [Google Scholar]
  6. Green H., Barrow P., Goldberg B. 1959; Effect of antibody and complement on permeability control in ascites tumor cells and erythrocytes. Journal of Experimental Medicine 110:699–713
    [Google Scholar]
  7. Madoff M.A., Cooper L.Z., Weinstein L. 1964; Hemolysis of rabbit erythrocytes by purified staphylococcal alpha-toxin. Journal of Bacteriology 87:145–149
    [Google Scholar]
  8. Manery J.F. 1969; Calcium and membranes. In Mineral Metabolism, 3: pp. 405–452 New York: Academic Press;
    [Google Scholar]
  9. Poste G., Allison A.C. 1973; Membrane fusion. Biochimica et biophysica acta 300:421–465
    [Google Scholar]
  10. Scharmann W. 1976a; Formation and isolation of leucocidin fromPseudomonas aeruginosa. Journal of General Microbiology 93:283–291
    [Google Scholar]
  11. Scharmann W. 1976b; Purification and characterization of leucocidin fromPseudomonas aeruginosa. Journal of General Microbiology 93:292–302
    [Google Scholar]
  12. Wilbrandt W. 1941; Osmotischc Natur sogenannter nichtosmotischer Hämolysen (Kolloid-osmotische Hamolyse). Archiv für die gesamte Physiologie 245:22–52
    [Google Scholar]
  13. Woodin A.M. 1970; Staphylococcal leukocidin. In Microbial Toxins 3 pp. 327–355 Montie T. C., Kadis S., Ajl S. J. Edited by New York: Academic Press;
    [Google Scholar]
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