1887

Abstract

Summary: A toxic substance, which destroyed leucocytes from man but was inactive against erythrocytes, was demonstrated in cultures of four out of 110 strains of tested. The toxin, designated ‘leucocidin’, was cell-bound as a precursor toxin, exhibiting little or no toxicity. It was converted into toxin with maximum activity by various proteases including an endogenous elastase. The production of leucocidin was directly proportional to the number of bacteria and was not influenced by variations in media, iron concentration, pH or temperature. The best method for large-scale production of leucocidin was autolysis of washed bacteria.

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1976-04-01
2024-04-23
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References

  1. Balke E., Scharmann W. 1974; Untcrsuchungen über eine Protease (Elastase) ausPseudomonas aeruginosa. II. Characterisierung des Enzyms. Hoppe-Seyler’s Zeitschrift für physiologische Chemie 355:958–968
    [Google Scholar]
  2. Bernheimer A.W., Schwartz L.L. 1963; Isolation and composition of staphylococcal alpha toxin. Journal of General Microbiology 30:561–568
    [Google Scholar]
  3. Brown J.A., West W.L., Banks W.M., Marshall J.D. 1970; Some biological effects of a heat labile toxin fromPasteurella pseudotuberculosis. Cytobios 2:199–205
    [Google Scholar]
  4. Dubos R.J., Geiger J.W. 1946; Preparation and properties of shiga toxin and toxoid. Journal of Experimental Medicine 84:143–156
    [Google Scholar]
  5. Dulbecco R., Vogt M. 1954; One-step growth curve of western equine encephalomyelitis virus chicken embryo cells grownin vitro and analysis of virus yield from single cells. Journal of Experimental Medicine 99:183–199
    [Google Scholar]
  6. Farmer J.J.III Herman L.G. 1969; Epidemiological fingerprinting ofPseudomonas aeruginosa by the production of and sensitivity to pyocin and bacteriophage. Applied Microbiology 18:760–765
    [Google Scholar]
  7. Foley F.D., Greenawald K.A., Nash G., Pruitt B.A. 1969; Pathology of Pseudomonas infection. Texas Medicine 63:36–39
    [Google Scholar]
  8. Fraenkel E. 1912; Über die Menschenpathogenität desBacillus pyocyaneus. Zeitschrift für Hygiene und Infektionskrankheiten 72:486–522
    [Google Scholar]
  9. Gladstone G.P., van Heyningen W.E. 1957; Staphylococcal leucocidins. British Journal of Experimental Pathology 38:123–137
    [Google Scholar]
  10. Heilmann H.D. 1972; On the peptidoglycan of the cell walls ofPseudomonas aeruginosa. European Journal of Biochemistry 31:456–463
    [Google Scholar]
  11. Helm E., Stille W. 1972; Klinik und Therapie der Sepsis durchPseudomonas aeruginosa. Deutsche medizinische Wochenschrift 97:1584–1589
    [Google Scholar]
  12. Hubbard J.D., King H., Beamer P.R. 1957; The pathogenicity ofPseudomonas aeruginosa. Experimental studies and report of a case. American Journal of Clinical Pathology 28:243–257
    [Google Scholar]
  13. Hugh R. 1970; Pseudomonas and Aeromonas. In Manual of Clinical Microbiology pp. 175–190 Blair J. E., Lenette E. H., Truant J. P. Edited by Bethesda, Maryland: American Society for Microbiology;
    [Google Scholar]
  14. Kanoe M., Blobel H., Schaeg W. 1969; Selektive Anreicherung von Staphylokokken-Panton-Valentine-Leukozidin mit Aluminium-Cholesterin. Zeitschrift für Naturforschung 24b:1431–1435
    [Google Scholar]
  15. Lamanna C., Sakaguchi G. 1971; Botulinal toxins and problem of nomenclature of simple toxins. Bacteriological Reviews 35:242–249
    [Google Scholar]
  16. Liu P.V. 1964; Factors that influence toxogenicity of Pseudomonas aeruginosa. Journal of Bacteriology 88:1421–1427
    [Google Scholar]
  17. Liu P.V. 1974; Extracellular toxins of Pseudomonas aeruginosa. Journal of Infectious Diseases 130:S94–S99
    [Google Scholar]
  18. Lowry O. H., Rosebrough N. T., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  19. Martinek R.G., Berger L., Broida D. 1964; Simplified estimation of leucine aminopeptidase activity. Clinical Chemistry 10:1087–1097
    [Google Scholar]
  20. Matheson A.T. 1963; The localization and properties of an aminopeptidase in Escherichia coli B. Canadian Journal of Biochemistry 41:9–18
    [Google Scholar]
  21. Moncrief J.A., Teplitz C. 1964; Changing concepts in burn sepsis. Journal of Trauma 4:233–245
    [Google Scholar]
  22. Morihara K., Tsuzuki H., Oka T., Inoue H., Ebata M. 1965; Pseudomonas aeruginosa elastase: Isolation, crystallisation, and preliminary characterization. Journal of Biological Chemistry 240:3295–3304
    [Google Scholar]
  23. Pappenheimer A.M., Johnson S.J. 1936; Studies in diphtheria toxin production. I. The effect of copper and iron. British Journal of Experimental Medicine 17:335–341
    [Google Scholar]
  24. Pavlovskis O.R., Gordon F.B. 1972; Pseudomonas aeruginosa exotoxin: effect on cell cultures. Journal of Infectious Diseases 125:631–636
    [Google Scholar]
  25. Pflüger R., Scharmann W., Blobel H. 1975; Toxicity of culture supernatants from Pseudomonas aeruginosa. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I) (in the Press).
    [Google Scholar]
  26. Pulverer G., Korth H. 1961; Zur Biochemie und Fermentstruktur von Pseudomonas aeruginosa. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I) 186:36–44
    [Google Scholar]
  27. Sbarra A.J., Gilfillan R.E., Bardawil W.A. 1960; A plate assay for elastase. Nature; London: 188322–323
    [Google Scholar]
  28. Schaffer A.J., Oppenheimer E.H. 1948; Pseudomonas (Pyocyaneus) infection of the gastrointestinal tract in infants and children. Southern Medical Journal 41:460–466
    [Google Scholar]
  29. Scharmann W., Balke E. 1974; Untersuchungen iiber eine Protease (Elastase) von Pseudomonas aeruginosa. I. Bildung und Reinigung der Protease. Hoppe-Seyler’s Zeitschrift für physiologische Chemie 355:443–450
    [Google Scholar]
  30. Scharmann W., Jacob F., Porstendürfer J. 1976; The cytotoxic action of leucocidin irom Pseudomonas aeruginosa on human polymorphonuclear leucocytes. Journal of General Microbiology 93:303–308
    [Google Scholar]
  31. Seki T., Takaki M., Hirabayashi H., Yamaguchi H. 1954; Toxin production by autolysis of Clostridium tetani. Medical Journal of Osaka University 5:271–289
    [Google Scholar]
  32. Sensakovic J.W., Bartell P.F. 1974; The slime oiPseudomonas aeruginosa: biological characterization and possible role in experimental infection. Journal of Infectious Diseases 129:101–109
    [Google Scholar]
  33. Teplitz C. 1965; Pathogenesis of Pseudomonas vasculitis and septic lesions. Archives of Pathology 80:297–307
    [Google Scholar]
  34. Turner A.W., Rodwell A.W. 1943; The epsilon toxin of Clostridium welchii type D. I. Proteolytic conversion of epsilon protoxin into episilon toxin by trypsin and other proteases. Australian Journal of Experimental Biology and Medical Sciences 21:17–25
    [Google Scholar]
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