@article{mbs:/content/journal/micro/10.1099/00221287-93-1-82, author = "Spencer, Rosemary and Cross, G. A. M.", title = "Lability of RNA from the Large Cytoplasmic Ribosomal Subunit of the Protozoon Crithidia oncopelti", journal= "Microbiology", year = "1976", volume = "93", number = "1", pages = "82-88", doi = "https://doi.org/10.1099/00221287-93-1-82", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-93-1-82", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Summary: Cytoplasmic ribosomal RNA extracted from Crithidia oncopelti and analysed by gel electrophoresis at 4 °C consisted of two components, with molecular weights (relative to E. coli rRNA) of 1·30 × 106 and 0·83 × 106 daltons, present in equimolar amounts. On heating briefly at 51 followed by rapid cooling, the 1·30 × 106 RNA completely dissociated into two components of molecular weights 0·70 × 106 and 0·56 × 106 (present in equimolar amounts). Fifty per cent dissociation of the molecule occurred at 28. That the integrity of the RNA molecule at low temperatures is maintained by its secondary structure was confirmed by electrophoresis under denaturing conditions (98%, v/v, formamide). To account for these phenomena, latent cleavage of the molecule in vivo is proposed.", }