1887

Abstract

SUMMARY: Cytoplasmic ribosomal RNA extracted from and analysed by gel electrophoresis at 4 ° consisted of two components, with molecular weights (relative to rRNA) of 1.30 x 10 and 0.83 x 10 daltons, present in equimolar amounts. On heating briefly at 51 ° followed by rapid cooling, the 1.30 x 10 RNA completely dissociated into two components of molecular weights 0.70 x 10 and 0.56 x 10 (present in equimolar amounts). Fifty per cent dissociation of the molecule occurred at 28 °. That the integrity of the RNA molecule at low temperatures is maintained by its secondary structure was confirmed by electrophoresis under denaturing conditions (98%, v/v, formamide). To account for these phenomena, latent cleavage of the molecule is proposed.

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/content/journal/micro/10.1099/00221287-93-1-82
1976-03-01
2019-10-22
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-93-1-82
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