1887

Abstract

Summary: The extent of the deletions of five Tet Tra mutants of R100-1 was determined by complementation experiments with wild-type and mutants of F The presence or absence of the origin of transfer on the mutants was also investigated. Using the results, a tentative map of this region of the R factor was drawn: it was essentially similar to the analogous region of the K12 F factor, except that was located between and

Some of the deletions had removed the promoter for the transfer operon. This allowed detection of the transcription of and distal genes from a weak, -independent promoter. This is probably the Is2 promoter, since R100-1 carries an Is2 insertion sequence located immediately to the left of in the correct orientation. Since neither the transfer operon promoter nor the Is2 promoter seemed to be required for transcription of , it was concluded that, unlike the F factor, this was located in a separate operon.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-93-1-133
1976-03-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/93/1/mic-93-1-133.html?itemId=/content/journal/micro/10.1099/00221287-93-1-133&mimeType=html&fmt=ahah

References

  1. Achtman M., Willetts N. S., Clark A. J. 1971; Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transferdeficient mutants. Jorrrnal of Bacteriology 106:529–538
    [Google Scholar]
  2. Achtman M., Willetts N. S., Clark A. J. 1972; Conjugational complementation analysis of transferdeficient mutants of F lac in Escherichia coli. Journal of Bacteriology 110:831–842
    [Google Scholar]
  3. Clowes R. C., Hayes W. 1968; Experiments in Microbial Genetics. Oxford and Edinburgh: Blackwell Scientific Publications;
    [Google Scholar]
  4. Egawa R., Hirota Y. 1962; Inhibition of fertility by multiple-drug-resistance factor (R) in Escherichia coli K12. Japanese Journal of Genetics 37:66–69
    [Google Scholar]
  5. Finnegan D., Willetts N. S. 1973; The site of action of the F transfer inhibitor. Molecular and General Genetics 127:307–316
    [Google Scholar]
  6. Foster T. J. 1975; Tetracycline-sensitive mutants of the F-like R factors R100 and R-1. Molecular and General Genetics 137:85–88
    [Google Scholar]
  7. Foster T. J., Howe T. G. B. 1973; Deletion map of the chloramphenicol resistance region of Ri and R100–1. Journal of Bacteriology 116:1062–1063
    [Google Scholar]
  8. Foster T. J., Walsh A. 1974; Phenotypic characterization of R-factor tetracycline resistance determinants. Genetical Research 24:333–343
    [Google Scholar]
  9. Hashimoto H., Hirota H. 1966; Gene recombination and segregation of resistance transfer factor R in Escherichia coli. Journal of Bacteriology 91:51–62
    [Google Scholar]
  10. Helmuth R., Achtman M. 1974; The F factor carries an operon of more than 15 × 106 daltons coding for DNA transfer and surface exclusion. Microbiology 1:95–103
    [Google Scholar]
  11. Hu S., Ohtsubo E., Davidson N., Saedler H. 1975; Electron microscope heteroduplex studies of sequence relations among bacterial plasmids: identification and mapping of the insertion sequences Is1 and Is2 in F and R plasmids. Journal of Bacteriology 122:764–775
    [Google Scholar]
  12. Ippen-Ihler K., Achtman M., Willetts N. S. 1972; Deletion map of the E. coli. K12 sex factor F: the order of eleven transfer cistrons. Journal of Bacteriology 110:857–863
    [Google Scholar]
  13. Ohtsubo E., Nishimura Y., Hirota Y. 1970; Transfer-defective mutants of sex factors in E. coli. I. Defective mutants and complementation analysis. Genetics 64:173–188
    [Google Scholar]
  14. Reeves P., Willetts N. S. 1974; Plasmid specificity of the origin of transfer of sex factor F:. Journal of Bacteriology 120:125–130
    [Google Scholar]
  15. Sharp P. A., Cohen S. N., Davidson N. 1973; Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors. Journal of Molecular Biology 75:235–255
    [Google Scholar]
  16. Sharp P. A., Hsu M., Ohtsubo E., Davidson N. 1972; Electron microscope heteroduplex studies of sequence relations among plasmids of E. coli. I. Structure of F-prime factors. Journal of Molecular Biology 71:471–497
    [Google Scholar]
  17. Willetts N. S. 1971; Plasmid specificity of two proteins required for conjugation in E. coli K12. Nature New Biology 230:183–185
    [Google Scholar]
  18. Willetts N. S. 1972; Location of the origin of transfer of the sex factor F. Journal of Bacteriology 112:773–778
    [Google Scholar]
  19. Willetts N. S. 1974; Mapping loci for surface exclusion and incompatibility on the F factor of Escherichia coli K-12. Journal of Bacteriology 118:778–782
    [Google Scholar]
  20. Willetts N. S., Achtman M. 1972; Genetic analysis of transfer by the Escherichia coli sex factor F, using P1 transductional complementation. Journal of Bacteriology 110:843–851
    [Google Scholar]
  21. Willetts N. S., Maule J. 1974; Interactions between the surface exclusion systems of some F-like plasmids. Genetical Research 24:81–89
    [Google Scholar]
  22. Yoshikawa M. 1974; Identification and mapping of the replication genes of an R factor, R100–1, integrated into the chromosome of Escherichia coli K-12. Journal of Bacteriology 118:1123–1131
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-93-1-133
Loading
/content/journal/micro/10.1099/00221287-93-1-133
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error