1887

Abstract

Summary

A requirement of choline for the growth of in a simplified culture medium has been demonstrated. Ethanolamine, -methylethanolamine, or -dimethylethanolamine were ineffective as substitutes. In the rumen, the normal environment of this organism, levels of free choline were virtually zero even after ingestion of pasture containing phosphatidylcholine which was rapidly catabolized. Free [ -C]choline is very rapidly cleared from rumen fluid, a little being incorporated into the phosphatidylcholine of protozoa, but the clearance also occurs in animals with defaunated rumens. It is suggested that obtains choline for growth mainly from plant membrane material which it has ingested, rather than from the free base in the rumen liquor.

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/content/journal/micro/10.1099/00221287-92-2-391
1976-02-01
2024-12-13
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References

  1. Broad T. E., Dawson R. M. C. 1975; Phospholipid biosynthesis in the anaerobic protozoon Entodinium caudatum. Biochemical Journal 146:317–328
    [Google Scholar]
  2. Coleman G. S. 1962; The preparation and survival of almost bacteria-free suspensions of Entodinium caudatum. Journal of General Microbiology 28:271–281
    [Google Scholar]
  3. Dawson R. M. C. 1959; Hydrolysis of lecithin and lysolecithin by rumen micro-organisms of the sheep. Nature; London: 1831822–1823
    [Google Scholar]
  4. Dawson R. M. C., Hemington N. 1974; Digestion of grass lipids and pigments in the sheep rumen. British Journal of Nutrition 32:327–340
    [Google Scholar]
  5. Dawson R. M. C., Hemington N., Davenport J. B. 1962; Improvements in the method of determining individual phospholipids in a complex mixture by successive chemical hydrolyses. Biochemical Journal 84:497–501
    [Google Scholar]
  6. Dawson R. M. C., Kemp P. 1967; The aminoethylphosphonate-containing lipids of rumen protozoa. Biochemical Journal 105:837–842
    [Google Scholar]
  7. Dawson R. M. C., Kemp P. 1969; The effect of defaunation on the phospholipids and on the hydrogenation of unsaturated fatty acids in the rumen. Biochemical Journal 115:351–352
    [Google Scholar]
  8. Eadie I., Hebb C., Mann S. P. 1973; Free choline levels in the rat brain. Journal of Neurochemistry 20:1499–1502
    [Google Scholar]
  9. Hall F. J., West J., Coleman G. S. 1974; Fine structural studies on the digestion of chloroplasts in the rumen ciliate Entodinium caudatum. Tissue and Cell 6:243–253
    [Google Scholar]
  10. Hazlewood G. P., Dawson R. M. C. 1975; Isolation and properties of a phospholipid-hydrolysing bacterium from ovine rumen fluid. Journal of General Microbiology 89:163–174
    [Google Scholar]
  11. Hyden S. 1955; A turbidimetric method for the determination of higher polyethylene glycols in biological materials. Lantbrukshögskolans Annaler 22:139–145
    [Google Scholar]
  12. Whelan W. J. 1955; Starch, glycogen, fructosans and similar polysaccharides. In Modern Methods of Plant Analysis 2 pp. 145–196 Paech K., Tracey M. V. Edited by Berlin: Springer-Verlag;
    [Google Scholar]
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