Genetic Transfer of O Antigens to 08 Free

Abstract

Summary: His hybrids from a cross between a donor and an O8 recipient expressed O8 specificity and in addition Salmonella O4,12-specificity. This indicated that the recipients had received the -linked donor cluster determining the synthesis of O-specific repeat units and that the genes of both mating partners are functional in these hybrids. Chemical analyses showed that the hybrids contained an O8 lipopolysaccharide (0 antigen) and a specific lipopolysaccharide with only one O-specific repeat unit (SR antigen).

O8-negative mutants selected from the O8-positive hybrids retained the Salmonella O-specificity and represent semi-rough (SR) forms, because the gene(s) determining the polymerization of repeat units has not been transferred. Attempts to introduce the locus into O8 remained unsuccessful.

Crosses between a donor and O8 gave rise to smooth (S) and SR His recombinants exhibiting only O-specificity. The smooth recombinants are assumed to have obtained the -linked cluster and in addition the gene(s) of the donor. The exchange of the region of such smooth recombinants by that of a donor led to smooth hybrids with O4,(5), 12-specificity.

The phenotypically smooth recombinants exhibited concomitantly S-and SR-lipopolysaccharides of and O-specificity, respectively.

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1976-02-01
2024-03-28
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