1887

Abstract

Summary: His hybrids from a cross between a donor and an O8 recipient expressed O8 specificity and in addition Salmonella O4,12-specificity. This indicated that the recipients had received the -linked donor cluster determining the synthesis of O-specific repeat units and that the genes of both mating partners are functional in these hybrids. Chemical analyses showed that the hybrids contained an O8 lipopolysaccharide (0 antigen) and a specific lipopolysaccharide with only one O-specific repeat unit (SR antigen).

O8-negative mutants selected from the O8-positive hybrids retained the Salmonella O-specificity and represent semi-rough (SR) forms, because the gene(s) determining the polymerization of repeat units has not been transferred. Attempts to introduce the locus into O8 remained unsuccessful.

Crosses between a donor and O8 gave rise to smooth (S) and SR His recombinants exhibiting only O-specificity. The smooth recombinants are assumed to have obtained the -linked cluster and in addition the gene(s) of the donor. The exchange of the region of such smooth recombinants by that of a donor led to smooth hybrids with O4,(5), 12-specificity.

The phenotypically smooth recombinants exhibited concomitantly S-and SR-lipopolysaccharides of and O-specificity, respectively.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-92-2-311
1976-02-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/92/2/mic-92-2-311.html?itemId=/content/journal/micro/10.1099/00221287-92-2-311&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1959 Bacteriophages pp. 461–469 New York: Interscience Publishers;
    [Google Scholar]
  2. Baron L. S., Gemski P., Johnson E. M., Wohlhieter J. H. 1968; Intergeneric bacterial mating. Bacteriological Reviews 32:362–369
    [Google Scholar]
  3. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1955; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  4. Hämmerling G., Lüderitz O., Westphal O., Mäkelä P. H. 1971; Structural investigations on the core polysaccharide of Escherichia coli O100. European Journal of Biochemistry 22:331–344
    [Google Scholar]
  5. Holme T., Lindberg A. A., Garegg P. J., Onn T. 1968; Chemical composition of cell wall polysaccharides of rough mutants from Salmonella typhimurium. Journal of General Microbiology 52:45–54
    [Google Scholar]
  6. Jann K., Schmidt G., Wallenfels B., Friund-Mölbert E. 1971; Isolation and characterization of Escherichia coli bacteriophage 08 specific for E. coli strains belonging to sero-group O8. Journal of General Microbiology 67:289–297
    [Google Scholar]
  7. Johnson E. M., Baron L. S. 1969; Genetic transfer of the Vi antigen from Salmonella typhosa to Escherichia coli. Journal of Bacteriology 99:358–359
    [Google Scholar]
  8. Johnson E. M., Falkow S., Baron L. S. 1964; Chromosome transfer kinetics of Salmonella Hfr strains. Journal of Bacteriology 88:395–400
    [Google Scholar]
  9. Jones R. T., Koeltzow E. E., Stocker B. A. D. 1972; Genetic transfer of Salmonella typhimurium and Escherichia coli lipopolysaccharide antigens to Escherichia coli K12. Journal of Bacteriology 111:758–770
    [Google Scholar]
  10. Kallings L. O. 1967; Sensitivity of various Salmonella strains to Felix O-1 phage. Acta pathologica et microbiologica Scandinavia 70:446–460
    [Google Scholar]
  11. Kauffmann F. 1966 The Bacteriology of Enterobacteriaceae Copenhagen: Munksgaard;
    [Google Scholar]
  12. Kay D. 1962; The nucleic acid composition of bacteriophage ɸR. Journal of General Microbiology 27201–207
    [Google Scholar]
  13. Kiefer W. 1974 Salmonella typhimurium Escherichia coli Rekombinanten Thesis, Freiburg University
    [Google Scholar]
  14. Lindberg A. A. 1967; Studies of a receptor for Felix O-1 phage in Salmonella minnesota. Journal of General Microbiology 48225–233
    [Google Scholar]
  15. Lindberg A. A. 1973; Bacteriophage receptors. Annual Review of Microbiology 27:205–241
    [Google Scholar]
  16. Lüderitz O., Westphal O., Staub A. M., Nikaido H. 1971; isolation and chemical and immunological characterization of bacterial lipopolysaccharides. In Microbial Toxins 4 pp. 145–224 Weinbaum G., Kadis S., Ajl S. J. Edited by New York: Academic Press;
    [Google Scholar]
  17. Mäkelä P. H. 1965; Inheritance of the G antigens of Salmonella groups B and D. Journal of General Microbiology 41:57–65
    [Google Scholar]
  18. Mäkelä P. H. 1966; Genetic determinants of the O antigens of Salmonella groups B(4,5,12) and C2(6,7). Journal of Bacteriology 91:1115–1125
    [Google Scholar]
  19. Mäkelä P. H. 1973; Glucosylation of lipopolysaccharide in Salmonella mutants negative for O antigen factor 122. Journal of Bacteriology 116:847–856
    [Google Scholar]
  20. Mayer H., Schmidt G. 1973; The occurrence of three different lipopolysaccharide cores in Shigella and their relationship to known enterobacterial core types. Zentralblatt für Bakteriologie, Parasitenkunde, Infecktionskrankheiten und Hygiene(Abteilung 1) 224:345–354
    [Google Scholar]
  21. Mojica-A T., Middleton R. B. 1972; Salmonella typhimurium-Escherichia coli hybrids for the tryptophan region. Genetics 71:491–505
    [Google Scholar]
  22. Müller-Seitz E., Jann B., Jann K. 1968; Degradation studies on the lipopolysaccharide from E. coli O71:K?:H12. Separation and investigation of O-specific and core polysaccharide. FEBS Letters 1:311–314
    [Google Scholar]
  23. Ørskov F., Ørskov I. 1962; Behaviour of Escherichia coli antigens in sexual recombination. Acta pathologica microbiologica Scandinavia 55:99–109
    [Google Scholar]
  24. Reske K., Jann K. 1972; The O8 antigen of Escherichia coli. Structure of the polysaccharide chain. European Journal of Biochemistry 31:320–328
    [Google Scholar]
  25. Rudén U., Mäkelä P. H. 1974; O-Acetylation and glucosylation of lipopolysaccharide in hybrids between Salmonella groups B and C2. European Journal of Biochemistry 48:11–20
    [Google Scholar]
  26. Sanderson K. E., Ross H., Ziegler L., Mäkelä P. H. 1972; F+, Hfr and F′ strains of Salmonella typhimurium and Salmonella abony. Bacteriological Reviews 36:608–637
    [Google Scholar]
  27. Schlecht S., Westphal O. 1966; Wachstum und Lipopolysaccharid (O-Antigen)-Gehalt von Salmonellen bei Züchtung auf Agarnährböden. Zentralblatt für Bakteriologie, Parasitenkunde, Infecktionskrankheiten und Hygiene(Abteilung I) 200:241–259
    [Google Scholar]
  28. Schmidt G. 1973; Genetical studies on the lipopolysaccharide structure of Escherichia coli K12. Journal of General Microbiology 77:151–160
    [Google Scholar]
  29. Schmidt G., Jann B., Jann K. 1969; Immunochemistry of R lipopolysaccharides of Escherichia coli. Different core regions in the lipopolysaccharides of O group 8. European Journal of Biochemistry 10:501–510
    [Google Scholar]
  30. Schmidt G., Jann B., Jann K. 1970; Studies on R mutants with an incomplete core, derived from Escherichia coli O8:K27. European Journal of Biochemistry 16:382–392
    [Google Scholar]
  31. Schmidt G., Jann B., Jann K. 1974; Genetic and immunochemical studies on Escherichia coli O14.K7: H. European Journal of Biochemistry 42:303–309
    [Google Scholar]
  32. Staub A. M., Tinelli R., Löderitz O., Westphal O. 1959; Rôle de quelques sucres et en particulier des 3,6-didesoxyhexoses dans la spécificité des antigènes O du tableau Kauffmann-White. Annales de l’Institut Pasteur 96:303–332
    [Google Scholar]
  33. Stocker B. A. D., Mäkelä P. H. 1971; Genetic aspects of biosynthesis and structure of Salmonella lipopolysaccharide. In Microbial Toxins 4 pp. 369–433 Weinbaum G., Kadis S., Ajl S. J. Edited by New York: Academic Press;
    [Google Scholar]
  34. Strominger J. L., Park T. J., Thompson R. E. 1959; Composition of the cell wall of Staphylococcus aureus: its relation to the mechanism of action of penicillin. Journal of Biological Chemistry 234:3263–3368
    [Google Scholar]
  35. Waravdekar V. S., Saslaw L. D. 1959; A sensitive colorimetric estimation of 2-deoxysugars with use of the malonaldehydethiobarbituric acid reaction. Journal of Biological Chemistry 234:1945–1950
    [Google Scholar]
  36. Wilkinson R. G., Gemski P., Stocker B. A. D. 1972; Non-smooth mutants of Salmonella typhimurium.Differentiation by phage sensitivity and genetic mapping. Journal of General Microbiology 70:527–554
    [Google Scholar]
  37. Zinder N. D. 1953; Infective heredity in bacteria. Cold Spring Harbor Symposia on Quantitative Biology 18:261–269
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-92-2-311
Loading
/content/journal/micro/10.1099/00221287-92-2-311
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error