1887

Abstract

SUMMARY: A mycelial suspension of (ATCC24938), grown on glucose-peptone-yeast extract broth and exposed to NaHCO for 5 h, fixed significant quantities of C into the following fractions (%): small molecular weight components, 74; lipid and lipoproteins, 3·9; nucleic acids, 59; the residual protein and cell wall fragments, 29·2. The labelled protein components were (%): aspartate, 39; glutamate, 18; cystine, 15; threonine, 9. Radioactive nucleic acid components were (%): adenine, 18; guanine, 18; cytidylate, 34; uridylate, 30. When the mycelium was grown in Czapek-Dox glucose medium and incubated in this medium plus NaHCO, the nucleic acid fraction contained 29·9% and the residual protein 49·5% of the cellular radioactivity. The removal of CO from the atmosphere did not reduce growth. Pyruvate carboxylase (PC) and phosphoenolpyruvate carboxykinase (PEPCK) activities were demonstrated in extracts of . Synthesis of PEPCK was stimulated under conditions promoting gluconeogenesis and was reduced under conditions promoting glycolysis, while PC synthesis was similar under both conditions.

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1976-02-01
2021-05-15
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References

  1. Bartnicki-Garcia S. 1963; Symposium on biochemical basis of morphogenesis in fungi. III. Mold-yeast dimorphism of Mucor. Bacteriological Reviews 27:293–304
    [Google Scholar]
  2. Block R. J. 1950; Estimation of amino acids and amines on paper chromatograms. Analytical Chemistry 22:1327–1332
    [Google Scholar]
  3. Block R. J. 1952 Paper Chromatography, a Laboratory Manual New York: Academic Press;
    [Google Scholar]
  4. Bloom S. J., Johnson M. J. 1962; The pyruvate carboxylase of Aspergillus niger. Journal of Biological Chemistry 237:2718–2720
    [Google Scholar]
  5. Budd K. 1969; The assimilation of bicarbonate by Neocosmospora vasinfecta. Canadian Journal of Microbiology 15:389–398
    [Google Scholar]
  6. Clark J. M. 1964 Experimental Biochemistry p. 17 San Francisco: W.H. Freeman;
    [Google Scholar]
  7. Drechsler C. 1923; Some graminicolous species of Helminthosporium. Journal of Agricultural Research 24:641–740
    [Google Scholar]
  8. Flavell R. B., Fincham J. R. S. 1968; Acetate-nonutilizing mutants of Neurospora crassa. II. Biochemical deficiencies and the roles of certain enzymes. Journal of Bacteriology 95:1063–1068
    [Google Scholar]
  9. Hardy T. L., Holland D. O., Nayler J. H. C. 1955; One phase solvent mixtures for the separation of amino acids. Analytical Chemistry 27:971–974
    [Google Scholar]
  10. Hartman R. E., Keen N. T. 1973; Enzymes capable of anaplerotic carbon dioxide fixation in Verticillium albo-atrum. Phytopathology 63:947–953
    [Google Scholar]
  11. Hartman R. E., Keen N. T. 1974a; The pyruvate carboxylase of Verticillium albo-atrum. Journal of General Microbiology 81:21–26
    [Google Scholar]
  12. Hartman R. E., Keen N. T. 1974b; The phosphoenolpyruvate carboxykinase of Verticillium albo-atrum. Journal of General Microbiology 81:21–26
    [Google Scholar]
  13. Hartman R. E., Keen N. T., Long M. 1972; Carbon dioxide fixation by Verticillium albo-atrum. Journal of General Microbiology 73:29–34
    [Google Scholar]
  14. Hofer M., Becker J., Betz A. 1970; Metabolism of the obligatory aerobic yeast Rhodotorula gracilis. II. Pyruvate kinase and phosphoenolpyruvate carboxykinase activities at various metabolic states. Archiv für Mikrobiologie 71:111–115
    [Google Scholar]
  15. Kirby K. S. 1955; Some new solvent systems for the paper chromatography of nucleic acid degradation products. Biochimica et biophysica acta 18:575–576
    [Google Scholar]
  16. Krebs H. A., Eggleston L. V. 1945; Metabolism of acetoacetate in a nimal tissues. Biochemical Journal 39:409–419
    [Google Scholar]
  17. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  18. Mahler H. R., Cordes E. H. 1971 Biological Chemistry, 2nd edn.. pp. 820–834 New York: Harper & Row;
    [Google Scholar]
  19. Nelson R. R. 1964; The perfect stage of Helminthosporium cynodontis. Mycologia 56:64–69
    [Google Scholar]
  20. Rick P. D., Mirocha C. T. 1968; Fixation of CO2 in the dark by the malic enzyme of bean and oat stem rust uredospores. Plant Physiology 43:201–207
    [Google Scholar]
  21. Roberts R. B., Abelson P. H., Cowie D. B., Bolton E. T., Britten R. J. 1955 Studies of Biosynthesis in Escherichia coli pp. 13–14 Publication No. 607. Washington, D.C.: Carnegie Institution;
    [Google Scholar]
  22. Roland J. F.Jr Gross A. M. 1954; Quantitative determination of amino acids using monodimen-sional paper chromatography. Analytical Chemistry 26:502–505
    [Google Scholar]
  23. Ruiz-Amil M., De Torrontegui G., Palacian E., Cataline L., Losada M. 1965; Properties and function of yeast pyruvate carboxylase. Journal of Biological Chemistry 240:3485–3492
    [Google Scholar]
  24. Stallknecht G. F., Mirocha C. J. 1971; Fixation and incorporation of CO2 into ribonucleic acid by germinating uredospores of Uromyces phaseoli. Phytopathology 61:400–405
    [Google Scholar]
  25. Stan H. J., Schormüller J. 1968; Regulation of pyruvate carboxylase of Penicillium camemberti by the adenylate system. Biochemical and Biophysical Research Communications 32:289–294
    [Google Scholar]
  26. Staples R. C., Weinstein L. H. 1959; Dark carbon dioxide fixation by uredospores of rust fungi.Contributions. Boyce Thompson Institute for Plant Research 20:71–82
    [Google Scholar]
  27. Tanaka K., Ono T., Yanagita T. 1966; Further observations on the carbon dioxide incorporation into RNA in the early phase of conidia germination in Aspergillus oryzae with special reference to soluble RNA synthesis. Journal of General and Applied Microbiology 12:329–336
    [Google Scholar]
  28. De Torrontegui G., Palacian E., Losada M. 1966; Phosphoenolpyruvate carboxykinase in gluconeo-genesis and its repression by hexoses in yeasts. Biochemical and Biophysical Research Communications 22:227–231
    [Google Scholar]
  29. Wang C. H., Willis D. L. 1965 Radiotracer Methodology in Biological Science Englewood Cliffs, New Jersey: Prentice-Hall;
    [Google Scholar]
  30. White J. P., Johnson G. T. 1971; Zinc effects on growth and cynodontin production of Helminthosporium cynodontis. Mycologia 63:548–561
    [Google Scholar]
  31. Woronick C. L., Johnson M. J. 1960; Carbon dioxide fixation by cell-free extracts of Aspergillus niger. Journal of Biological Chemistry 235:9–15
    [Google Scholar]
  32. Wyatt G. R. 1951; The purine and pyrimidine composition of deoxypentose nucleic acids. Biochemical Journal 48:584–590
    [Google Scholar]
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