1887

Abstract

SUMMARY: A mycelial suspension of (ATCC24938), grown on glucose-peptone-yeast extract broth and exposed to NaHCO for 5 h, fixed significant quantities of C into the following fractions (%): small molecular weight components, 74; lipid and lipoproteins, 3·9; nucleic acids, 59; the residual protein and cell wall fragments, 29·2. The labelled protein components were (%): aspartate, 39; glutamate, 18; cystine, 15; threonine, 9. Radioactive nucleic acid components were (%): adenine, 18; guanine, 18; cytidylate, 34; uridylate, 30. When the mycelium was grown in Czapek-Dox glucose medium and incubated in this medium plus NaHCO, the nucleic acid fraction contained 29·9% and the residual protein 49·5% of the cellular radioactivity. The removal of CO from the atmosphere did not reduce growth. Pyruvate carboxylase (PC) and phosphoenolpyruvate carboxykinase (PEPCK) activities were demonstrated in extracts of . Synthesis of PEPCK was stimulated under conditions promoting gluconeogenesis and was reduced under conditions promoting glycolysis, while PC synthesis was similar under both conditions.

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1976-02-01
2024-04-20
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