1887

Abstract

Summary: The ultrastructural characteristics of Neurospora cells during dedifferentiation and redifferentiation of conidiospores into vegetative cells have been determined. This germination process occurs between 2 and 5 h after inoculation; by 3·5 h, approximately 50 % of the cells have germinated. The cells enter the exponential phase of dry-weight gain between 4 and 5 h after inoculation. Several unusual structures are observed in Neurospora cells during germination. Whorled structures are frequently seen in the cytoplasm during germination, and occasionally at other times. They appear to be derived from the cytoplasmic membrane. Whorled structures of different appearance were observed in the mitochondria between 2 and 4 h after inoculation. Their number was related to the level of metabolizable carbohydrate, and was higher in 15 % glucose- than in 2 % sucrose-supplemented medium, and very low in medium containing 15 % mannitol, or 2 % sucrose + 13 % 2-deoxyglucose, or no added carbohydrate. The mitochondrial inclusions were osmiophilic and could be removed by treatment with 90 % aqueous acetone in the cold, indicating that they were composed at least in part of lipid. The strong dependence of the number of mitochondrial inclusins on time and on carbohydrate supplementation, suggests that there is a physiological basis for these structures and that they reflect changes occurring in the mitochondria at times significant to cellular differentiation.

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/content/journal/micro/10.1099/00221287-92-1-97
1976-01-01
2021-05-06
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References

  1. Ashworth C., Leonard J., Eigenbrodt E., Wrightsman F. 1966; Hepatic intracellular osmiopbillic droplets. Effect of lipid solvents during tissue preparation. Journal of Cell Biology 31:301–318
    [Google Scholar]
  2. Beck D. P., Greenawalt J. W. 1968; Factors affecting the formation of membranous structures in the cytoplasm and mitochondria of Neurospora crassa. Journal of Cell Biology 39:11A
    [Google Scholar]
  3. Beck D. P., Greenawalt J. W. 1976; Biogenesis of mitochondrial membranes in Neurospora crassa during cellular differentiation. II. Changes in oxidative phosphorylation and synthesis of mitochondrial phospholipids. Journal of General Microbiology 92:11–19
    [Google Scholar]
  4. Bianchi D., Turian G. 1967; Lipid content of conidia of Neurospora crassa. Nature; London: 2141344–1345
    [Google Scholar]
  5. Bruni C., Porter K. 1965; The fine structure of the parenchymal cells of the normal rat liver. I. General observations. American Journal of Pathology 46:691–755
    [Google Scholar]
  6. Cochrane V. W. 1966; Respiration and spore germination. In The Fungus Spore pp. 201–215 Madelin M. F. Edited by London: Butterworths;
    [Google Scholar]
  7. Curgy J. -J. 1968; Influence du mcde de fixation sur la possibilité d’observer des structures myeliniques dans les hépatocytes d’embryons de poulet. Journal de Microscopie 7:63–80
    [Google Scholar]
  8. Ericsson J., Biberfeld P. 1967; Studies on aldehyde fixation: fixation rates and their relation to fine structure and some histochemical relations in liver. Laboratory Investigation 7:281–298
    [Google Scholar]
  9. Greenawalt J. W. 1965; The effect of growth conditions on the cytology of Neurospora crassa. Journal of Cell Biology 27:37A
    [Google Scholar]
  10. Hall D. O., Greenawalt J. W. 1964; Oxidative phosphorylation by isolated mitochondria of Neurospora crassa. Biochemical and Biophysical Research Communications 17:565–569
    [Google Scholar]
  11. Hall D. O., Greenawalt J. W. 1967; The preparation and biochemical properties of mitochondria from Neurospora crassa. Journal of General Microbiology 48:419–430
    [Google Scholar]
  12. Hanks D., Sussman A. 1969; The relation between growth, conidiation and trehalase activity in Neurospora crassa. American Journal of Botany 56:1152–1159
    [Google Scholar]
  13. Luft J. H. 1961; Improvements in epoxy resin embedding methods. Journal of Biophysical and Biochemical Cytology 9:409–414
    [Google Scholar]
  14. Malhotra S. K. 1968; Mesosome-like structures of poky Neurospora. Nature; London: 2191267–1268
    [Google Scholar]
  15. Marzlue G., Metzenberg R. 1967; Studies on the functional significance of the transmembrane location of invertase in Neurospora crassa. Archives of Biochemistry and Biophysics 120:487–496
    [Google Scholar]
  16. Millonig G. 1961; Advantages of a phosphate buffer for OsO4 solutions in fixation. Journal of Applied Physiology 32:1637–1639
    [Google Scholar]
  17. Millonig G. 1962; Further observations on a phosphate buffer for osmium solutions. In Fifth International Congress for Electron Microscopy 2 p. 8 Breese S. S. Edited by New York: Academic Press;
    [Google Scholar]
  18. Moor H., Muhlethaler K. 1963; Fine structure in frozen-etched yeast cells. Journal of Cell Biology 17:609–628
    [Google Scholar]
  19. Neville M., Suskind S., Roseman S. 1971; A derepressible active transport system for glucose in Neurospora crassa. Journal of Biological Chemistry 246:1294–1301
    [Google Scholar]
  20. Perlman D. 1950; The metabolism of carbohydrates by Neurospora. Bulletin of the Torrey Botanical Club 77:103–109
    [Google Scholar]
  21. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17:208–212
    [Google Scholar]
  22. Sabatini D. D., Bensch K., Barnett R. J. 1963; Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. Journal of Cell Biology 17:19–58
    [Google Scholar]
  23. Stine G. J. 1967; Enzyme activities during the asexual cycle of Neurospora crassa. I. Succinic dehydrogenase. Canadian Journal of Microbiology 13:1203–1210
    [Google Scholar]
  24. Stine G. J. 1969; Investigations during phases of synchronous development and differentiation in Neurospora crassa. In The Cell Cycle pp. 119–139 Padilla G. M, Whitson G. L, Cameron I. L. Edited by New York: Academic Press;
    [Google Scholar]
  25. Theodore T. S., Panos C. 1973; Protein and fatty acid composition of mesosomal vesicles and plasma membranes of Staphylococcus aureus. Journal of Bacteriology 116:571–576
    [Google Scholar]
  26. Trevithick J., Metzenberg R. 1966; Molecular sieving by Neurospora cell walls during secretion of invertase isozymes. Journal of Bacteriology 92:1010–1015
    [Google Scholar]
  27. Turian G. 1966; Morphogenesis in ascomycetes. In The Fungi: An Advanced Treatise. II. The Fungal Organism pp. 339–385 Ainsworth G. C, Sussman A. S. Edited by New York: Academic Press;
    [Google Scholar]
  28. Vogel H. J. 1956; A convenient growth medium for Neurospora (medium N). Microbiology and Genetics Bulletin 13:42–43
    [Google Scholar]
  29. Weiss B. 1965; An electron microscope and biochemical study of Neurospora crassa during development. Journal of General Microbiology 39:85–94
    [Google Scholar]
  30. Zalokar M. 1959; Enzyme activity and cell differentiation in Neurospora. American Journal of Botany 46:555–559
    [Google Scholar]
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