SUMMARY: The methyl esters of free mycolic acids from representative strains of and each gave, on mass spectro-scopy, homologous series of anhydromycolic esters containing from one to four double bonds with the main components of the parent mycolic acids centred on 56, 58, 62 or 64 carbon atoms (total range from C to C). The mycolic acids from the Gordona strains, with chain lengths centred around C, form a group intermediate in size between nocardomycolic acids (centred around C) and mycolic acids (centred around C to C); these are different from those of the complex which have anhydromycolates ranging from C to C. Gordonae are thus more closely related in their mycolic acid composition to than to but can be distinguished from each of these genera.


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