1887

Abstract

Summary: A new temperature-sensitive mutant of , defective in cell division, was isolated after selection for tolerance to colicin E2. The mutant strain, 124, growing in either minimal or complex medium, commences filament formation immediately upon shift to high temperature. High densities of bacteria or the presence of 0·44 -sucrose prevents filament formation at 42 °C and division continues. Filament formation in the mutant is reversible and upon return to 29 °C the multinucleate filaments divide up into normal-sized bacteria by a series of rapid but sequential divisions. In the presence of chloramphenicol at 29 °C, 25% of these division sites are still expressed. A genetic locus designated , apparently controlling both temperature sensitivity and filament formation, was provisionally mapped at minute 80 on the 12 map.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-92-1-156
1976-01-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/92/1/mic-92-1-156.html?itemId=/content/journal/micro/10.1099/00221287-92-1-156&mimeType=html&fmt=ahah

References

  1. Broda P., Meacock P., Achtman M. 1972; Early transfer of genes determining transfer functions by some Hfr strains in Escherichia coli. Molecular and General Genetics 116:336–347
    [Google Scholar]
  2. Buxton R. S. 1971; Genetic analysis of Escherichia coli K12 mutants resistant to bacteriophage BF23 and the E-group colicins. Molecular and General Genetics 113:154–156
    [Google Scholar]
  3. Buxton R. S., Holland I. B. 1973; Genetic studies of tolerance to colicin E2 in Escherichia coli K-12.I.Re-location and dominance relationship of cet mutations. Molecular and General Genetics 127:69–88
    [Google Scholar]
  4. Buxton R. S., Holland I. B. 1974; Genetics studies of tolerance to colicin E2 in Escherichia coli K-12.II. Multiple mutations as a cause of the various phenotypic properties of Cet mutants. Molecular and General Genetics 131:159–171
    [Google Scholar]
  5. Castellazzi M. J., George J., Buttin G. 1972; Prophage induction and cell division in Escherichia coli. Molecular and General Genetics 119:153–174
    [Google Scholar]
  6. Daniels M. J. 1969; Lipid synthesis in relation to the cell cycle of Bacillus megaterium KM and Escherichia coli. Biochemical Journal 115:697–701
    [Google Scholar]
  7. Donachie W. D., Jones N. C., Teather R. 1973; The bacterial cell cycle. Symposia of the Society for General Microbiology 23:9–44
    [Google Scholar]
  8. Hill C., Holland I. B. 1967; The genetic basis of colicin E sensitivity in Escherichia coli K-12. I. Isolation and properties of refractory mutants and the preliminary mapping of their mutations. Journal of Bacteriology 94:677–686
    [Google Scholar]
  9. Holland E. M., Holland I. B. 1970; Induction of DNA breakdown and inhibition of cell division by colicin E2. Nature of some early steps in the process and properties of the E2-specific nuclease system. Journal of General Microbiology 64:223–239
    [Google Scholar]
  10. Holland I. B. 1968; Properties of Escherichia coli K12 mutants which show conditional refractivity to colicin E2. Journal of Molecular Biology 31:267–275
    [Google Scholar]
  11. Holland I. B. 1975; Physiology of colicin action. Advances in Microbial Physiology 12:56–139
    [Google Scholar]
  12. Nagai K., Tamura G. 1972; Mutant of Escherichia coli with a thermosensitive protein in the process of cellular division. Journal of Bacteriology 112:959–966
    [Google Scholar]
  13. Normark S., Boman H. G., Bloom G. D. 1971; Cell division in a chain-forming envA mutant of Escherichia K12. Acta pathologica et microbiologica scandinavica B79:651–664
    [Google Scholar]
  14. Pierucci O., Helmstetter C. E. 1969; Chromosome replication, protein synthesis and cell division in Escherichia coli. Federation Proceedings 28:1755–1760
    [Google Scholar]
  15. Reeve J. N., Clark D. J. 1972; Cell division of Escherichia coli BUG-6. Effect of varying the length of growth at the non-permissive temperature. Journal of Bacteriology 110:117–121
    [Google Scholar]
  16. Reeve J. N., Groves N. G., Clark D. J. 1970; Regulation of cell division in Escherichia coli: characterization of temperature-sensitive division mutants. Journal of Bacteriology 104:1052–1064
    [Google Scholar]
  17. Ricard M., Hirota Y. 1973; Process of cellular division in Escherichia coli: physiological study on thermosensitive mutants defective in cell division. Journal of Bacteriology 116:314–322
    [Google Scholar]
  18. Russell R. R. B. 1972; Mapping of a d-cycloserine resistance locus in Escherichia coli K-12. Journal of Bacteriology 111:622–624
    [Google Scholar]
  19. Senior B. W., Kwasniak J., Holland I. B. 1970; Colicin E3-directed changes in ribosome function and polyribosome metabolism in Escherichia coli K12. Journal of Molecular Biology 53:205–220
    [Google Scholar]
  20. Slater M., Schaechter M. 1974; Control of cell division in bacteria. Bacteriological Reviews 38:199–221
    [Google Scholar]
  21. Stone A. B. 1973; Regulation of cell division in a temperature sensitive division mutant of Escherichia coli. Journal of Bacteriology 116:741–750
    [Google Scholar]
  22. Taylor A. L., Trotter C. D. 1967; Revised linkage map of Escherichia coli. Bacteriological Reviews 31:332–353
    [Google Scholar]
  23. Taylor A. L., Trotter C. D. 1972; Linkage map of Escherichia coli strain K-12. Bacteriological Reviews 36:504–524
    [Google Scholar]
  24. Threlfall E. J., Holland I. B. 1970; Co-transduction with serB of a pleiotropic mutation affecting colicin E2 refractivity, ultraviolet sensitivity, recombination proficiency and surface properties of Escherichia coli k12. Journal of General Microbiology 62:383–398
    [Google Scholar]
  25. Zusman D. R., Inouye M., Pardee A. B. 1972; Cell division in Escherichia coli: evidence for regulation of septation by effector molecules. Journal of Molecular Biology 69:119–138
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-92-1-156
Loading
/content/journal/micro/10.1099/00221287-92-1-156
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error