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Summary: The medium redox potential (E h) influenced the folate-limited growth of Lactobacillus casei; the growth response was maximal at an E h of +120 mV (pH 6·35). At raised E h serum folate would support less growth than pteroyl-glutamic acid, and the response to N 5-methyl tetrahydrofolic acid was intermediate between them. Pteroylglutamic acid was not destroyed during 24 h incubation at 37 °C in medium with E h values between +40 and +440 mV. Destruction of N 5-methyl tetrahydrofolic acid occurred within 24 h when the medium E h was greater than +125 mV. Folate was taken up rapidly by L. casei with an E h optimum at + 270 mV.
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