@article{mbs:/content/journal/micro/10.1099/00221287-91-2-383, author = "Coultate, T. P. and Sundaram, T. K. and Cazzulo, J. J.", title = "Stability of Protein and Ribonucleic Acid in Bacillus stearothermophilus", journal= "Microbiology", year = "1975", volume = "91", number = "2", pages = "383-390", doi = "https://doi.org/10.1099/00221287-91-2-383", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-91-2-383", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: The turnover of protein in a prototrophic strain of Bacillus stearothermophilus during exponential growth in a salts medium with glucose or succinate as carbon source was about 4 %/h and in a richer nutrient broth medium about 23 %/h. Protein degradation under non-growing conditions conformed to a similar pattern. The turnover of RNA (non-messenger) was about 1 %/h in salts medium and about 9 %/h in nutrient broth. The turnover of protein and RNA in the thermophile is thus moderate rather than massive. This conclusion was confirmed by measurement of the decay of a specific enzyme, isocitrate lyase, in the proto-troph and of the overall protein turnover in a non-prototrophic strain of B. stearothermophilus. The half-lives of a number of enzyme systems in intact cells of the prototrophic thermophile at its optimum growth temperature showed some variation but indicated a significant rate of inactivation. Such decay of protein in vivo apparently accounts for the moderate protein turnover observed during growth.", }