1887

Abstract

The mutagenic and lethal effects of -methyl--nitro--nitrosoguanidine (NTG), ethyl methanesulphonate (EMS), ultraviolet light irradiation and near-ultraviolet light irradiation with 8-methoxypsoralen on the bacterium NCIB8250 were examined. The production of auxotrophic mutants was used as a measure of mutagenic efficiency. Under appropriate conditions all four agents were mutagenic. EMS and NTG although more effective than irradiation, did not cause such a high frequency of mutation as has been observed with other bacteria. A combination of vancomycin and penicillin V gave enrichment of non-metabolizing bacteria and optimum conditions were found for the use of these compounds in a selection technique.

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/content/journal/micro/10.1099/00221287-91-2-338
1975-12-01
2019-10-20
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-91-2-338
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