1887

Abstract

SUMMARY: The mutagenic and lethal effects of -methyl--nitro--nitrosoguanidine (NTG), ethyl methanesulphonate (EMS), ultraviolet light irradiation and near-ultraviolet light irradiation with 8-methoxypsoralen on the bacterium 8250 were examined. The production of auxotrophic mutants was used as a measure of mutagenic efficiency. Under appropriate conditions all four agents were mutagenic. EMS and NTG although more effective than irradiation, did not cause such a high frequency of mutation as has been observed with other bacteria. A combination of vancomycin and penicillin V gave enrichment of non-metabolizing bacteria and optimum conditions were found for the use of these compounds in a selection technique.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-91-2-338
1975-12-01
2021-08-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/91/2/mic-91-2-338.html?itemId=/content/journal/micro/10.1099/00221287-91-2-338&mimeType=html&fmt=ahah

References

  1. Adelberg E. A., Mandel M., Chen G. C. C. 1965; Optimal conditions for mutagenesis by N-methyl- N′-nitro-N-nitrosoguanidine in Escherichia coli K12. Biochemical and Biophysical Research Communications 18:788–795
    [Google Scholar]
  2. CÁnovas J. L., Stanier R. Y. 1967; Regulation of the enzymes of the β-ketoadipate pathway in Moraxella calcoacetica. I. General aspects. European Journal of Biochemistry 1:289–300
    [Google Scholar]
  3. Delić V., Hopwood D. A., Friend E. J. 1970; Mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine (NTG) in Streptomyces coelicolor. Mutation Research 9:167–182
    [Google Scholar]
  4. Gale E. F., Cundliffe E., Reynolds P. E., Richmond M. H., Waring M. J. 1972 The Molecular Basis of Antibiotic Action. London: John Wiley.;
    [Google Scholar]
  5. Grant D. J. W. 1973; The degradative versatility, arylesterase activity and hydroxylation reactions of Acinetobacter Iwoffi N.C.I.B. 10553. Journal of Applied Bacteriology 36:47–59
    [Google Scholar]
  6. Herman N. J., Juni E. 1974; Isolation and characterisation of a generalised transducing bacteriophage for Acinetobacter. Journal of Virology 13:46–52
    [Google Scholar]
  7. Holliday R. 1956; A new method for the identification of biochemical mutants of micro-organisms. Nature; London: 178:987
    [Google Scholar]
  8. Hopwood D. A. 1970; The isolation of mutants. In Methods in Microbiology 3A pp. 363–433 Norris J. R., Ribbons D. W. London and New York: Academic Press.;
    [Google Scholar]
  9. Kennedy S. I. T., Fewson C. A. 1968; Enzymes of the mandelate pathway in bacterium N.C.I.B. 8250. Biochemical Journal 107:497–506
    [Google Scholar]
  10. Lederberg J., Lederberg E. M. 1952; Replica plating and indirect selection of bacterial mutants. Journal of Bacteriology 63:399–406
    [Google Scholar]
  11. Livingstone A., Fewson C. A., Kennedy S. I. T., Zatman L. J. 1972; Two benzaldehyde dehydrogenases in bacterium N.C.I.B. 8250. Distinguishing properties and regulation. Biochemical Journal 130:927–935
    [Google Scholar]
  12. Loveless A., Howarth S. 1959; Mutation of bacteria at high levels of survival by ethyl methane-sulphonate. Nature; London: 184:1780–1782
    [Google Scholar]
  13. Nečásek J., PikÁlek P., DrobnÁk J. 1966; The mutagenic effect of prolonged treatment with ethyl methanesulphonate. Mutation Research 4:409–413
    [Google Scholar]
  14. Perkins H. R. 1969; Specificity of combination between mucopeptide precursors and vancomycin or ristocetin. Biochemical Journal III:195–205
    [Google Scholar]
  15. Sawula R. V., Crawford I. P., Irving R. 1972; Mapping of the tryptophan genes of Acinetobacter calcoaceticus by transformation. Journal of Bacteriology 112:797–805
    [Google Scholar]
  16. Schlegel H. C., Jannasch H. W. 1967; Enrichment cultures. Annual Review of Microbiology 21:49–70
    [Google Scholar]
  17. Townsend M. E., Wright H. M., Hopwood D. A. 1971; Efficient mutagenesis by near-ultraviolet light in the presence of 8-methoxypsoralen in Streptomyces. Journal of Applied Bacteriology 34:799–800
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-91-2-338
Loading
/content/journal/micro/10.1099/00221287-91-2-338
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error