1887

Abstract

SUMMARY: A procedure to enrich for auxotrophic and fermentation mutants of is described. The method is based on the amplification of the killing power of carbenicillin by the addition of lysozyme. Isolation frequencies of some types of mutants are presented, with and without the application of the proposed procedure. The yield of mutants is usually enhanced a hundredfold per enrichment treatment.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-91-1-177
1975-11-01
2021-05-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/91/1/mic-91-1-177.html?itemId=/content/journal/micro/10.1099/00221287-91-1-177&mimeType=html&fmt=ahah

References

  1. Bourgin-Rosenberg M., Garnier-Expert D., Tourneur J. 1974; Obtention de mutants auxotrophes de deux souches d ’Agrobacterium tumefaciens: étude de leurs proprietes vis-a-vis du phage Ω. Comptes rendus hebdomadaire des seances de l’Académie des sciences 278:2791–2794
    [Google Scholar]
  2. Costerton J. W., Ingram J. M., Cheng K. J. 1974; Structure and function of the cell envelope of Gram-negative bacteria. Bacteriological Reviews 38:87–110
    [Google Scholar]
  3. Davis B. D. 1948; Isolation of biochemically deficient mutants of bacteria by penicillin. Journal of the American Chemical Society 70:4267
    [Google Scholar]
  4. Hamilton R. H., Fall M. Z. 1971; The loss of tumor-initiating ability in Agrobacterium tumefaciens by incubation at high temperature. Experientia 27:229–230
    [Google Scholar]
  5. Lederberg J., Zinder N. 1948; Concentration of biochemical mutants of bacteria with penicillin. Journal of the American Chemical Society 70:4267–4268
    [Google Scholar]
  6. Miller J. H. 1972 Experiments in Molecular Genetics. Cold Spring Harbor: Cold Spring Harbor Laboratories;
    [Google Scholar]
  7. Schilperoort R. A. 1969 Investigations on plant tumors: crown gall Thesis University of Leiden; The Netherlands:
    [Google Scholar]
  8. Vervliet G., Holsters M., Teuchy H., Van Montagu M., Schell J. 1975; Characterization of. different plaque forming and defective temperate phages in Agrobacterium strains. Journal of General Virology 26:33–48
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-91-1-177
Loading
/content/journal/micro/10.1099/00221287-91-1-177
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error