1887

Abstract

Summary: Mitochondrial structure in yeast cells under various physiological conditions has been studied by high voltage electron microscopy of sections that are 0·5 to 2·0 m thick. Such thick sections of the yeast had a small number of long, branched tubular mitochondria per cell. The mitochondria extended into cell buds and unseparated daughter cells. It was apparent from parallel studies with thin sections that most of the rounded mitochondrial profiles viewed in thin sections should not be interpreted as being numerous small individual mitochondria. Attempts to study thick sections of the yeasts and were frustrated by poor contrast.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-91-1-127
1975-11-01
2021-05-15
Loading full text...

Full text loading...

/deliver/fulltext/micro/91/1/mic-91-1-127.html?itemId=/content/journal/micro/10.1099/00221287-91-1-127&mimeType=html&fmt=ahah

References

  1. Arnold C. G., Schimmer O., Schotz F., Bathelt H. 1972; Die Mitochondrien von Chlamydomonas reinhardii. Arcftiv fiir Mikrobiologie 81:50–67
    [Google Scholar]
  2. Atkinson A. E., John P. C. L., Gunning B. E. S. 1974; The growth and division of the single mitochondrion and other organelles during the cell cycle of Chlorella studied by quantitative stereology and three dimensional reconstruction. Protoplasma 81:77–109
    [Google Scholar]
  3. Burton M. D., Moore J. 1974; The mitochondrion of the flagellate Polytomella agilis. Journal of Ultrastructure Research 48:414–419
    [Google Scholar]
  4. Dallman P. R., Goodman J. R. 1970; Enlargement of mitochondrial compartment in iron and copper deficiency. Blood 35:496–505
    [Google Scholar]
  5. Davison M. T., Downie J. A., Garland P. B. 1972; Giant mitochondria in iron- or copper-deficient Candida utilis. Biochemical Journal 129:46–47 p
    [Google Scholar]
  6. Davison M. T., Garland P. B. 1974; The intracellular morphology of yeast mitochondrial membranes: studies with high voltage electron microscopy. Proceedings of the Society for General Microbiology i:41
    [Google Scholar]
  7. Downie J. A., Garland P. B. 1973; An antimycin-A and cyanide-resistant variant of Candida utilis arising during copper limited growth. Biochemical Journal 134:1051–1061
    [Google Scholar]
  8. Favard P., Carasso N. 1973; The preparation and observation of thick biological sections in the high voltage electron microscope. Journal of Microscopy 97:59–81
    [Google Scholar]
  9. Garland P. B. 1970; A biochemical application of continuous culture: energy conservation in Torulopsis utilis. Biochemical Journal 118:329–339
    [Google Scholar]
  10. Gentsch P., Gilde H., Reimer L. 1974; Measurement of the top-bottom effect in scanning transmission electron microscopy of thick amorphous sections. Journal of Microscopy 100:81–92
    [Google Scholar]
  11. Glauert A. M. 1974; The high voltage electron microscope in biology. Journal of Cell Biology 63:717–748
    [Google Scholar]
  12. Haddock B. A., Garland P. B. 1971; Effect of sulphate-limited growth on mitochondrial electron transfer and energy conservation between reduced nicotinamide adenine dinucleotide and the cytochromes in Torulopsis utilis. Biochemical Journal 124:155–170
    [Google Scholar]
  13. Hoffmann H. P., Avers C. J. 1973; Mitochondrion of yeast: ultrastructural evidence for one giant, branched organelle per cell. Science; New York: 181749–751
    [Google Scholar]
  14. Kawakami N. 1961; Thread-like mitochondria in yeast cells. Experimental Cell Research 25:179–181
    [Google Scholar]
  15. Keddie F. M., Barajas L. 1969; Three-dimensional reconstitution of Pityrosporum yeast cells based on serial section electron microscopy. Journal of Ultrastructure Research 29:260–275
    [Google Scholar]
  16. Keyhani E. 1972; Localisation cytochimique de la cytochrome oxydase chez la levure Candida utilis. Journal de Microscopie 15:343–352
    [Google Scholar]
  17. Keyhani E. 1973; Morphological changes in yeast cell mitochondria grown at various copper concentrations. Experimental Cell Research 81:73–78
    [Google Scholar]
  18. Keyhani E., Chance B. 1971; Cytochrome synthesis under copper-limited conditions in Candida utilis. FEES Letters 17:127–132
    [Google Scholar]
  19. Light P. A. 1972; Mitochondrial effects of copper-limited growth of Torulopsis utilis. FEES Letters 19:319–322
    [Google Scholar]
  20. Light P. A., Garland P. B. 1971; A comparison of mitochondria from Torulopsis utilis grown in continuous culture with glycerol, iron, ammonium, magnesium or phosphate as the growth-limiting nutrient. Biochemical Journal 124:123–124
    [Google Scholar]
  21. Osafune T. 1973; Three-dimensional structure of giant mitochondria dictyosomes and concentric lamellar bodies formed during the cell cycle of Euglena gracilis (z) in synchronous culture. Journal of Electron Microscopy 22:51–61
    [Google Scholar]
  22. Osumi M., Miwa N., Teranishi Y., Tanaka A., Fukui S. 1974; Ultrastructure of Candida yeasts grown on n-alkanes,. Archiv fiir Mikrobiologie 99:181–201
    [Google Scholar]
  23. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron opaque stain in electron microscopy. Journal of Cell Eiology 17:209–212
    [Google Scholar]
  24. Rosen D., Edelman M., Galun E., Danon D. 1974; Biogenesis of mitochondria in Trichoderma viride: structural changes in mitochondria and other spore constituents during conidium maturation and germination. Journal of General Microbiology 83:31–49
    [Google Scholar]
  25. Seligman A. M., Karnovsky M. J., Wasserkrug L. H., Hanker J. S. 1968; Non-droplet ultrastructural demonstration of cytochrome oxidase activity with a polymerising osmiophilic reagent, diamino- benzidine. Journal of Cell Eiology 38:1–14
    [Google Scholar]
  26. Spurr A. R. 1969; A low-viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research 26:31–43
    [Google Scholar]
  27. Suzuki K. 1969; Giant mitochondria; production in mice fed with cuprizone. Science; New York: 16381
    [Google Scholar]
  28. Weibull C. 1974; Studies on thick sections of microorganisms using electron microscopes working at accelerating voltages from 60 to 1000 kV. Journal of Ultrastructure Research 47:106–114
    [Google Scholar]
  29. Yotsuyanagi Y. 1955; Mitochondria and refractive granules in the yeast cell. Nature; London: 1761208–1209
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-91-1-127
Loading
/content/journal/micro/10.1099/00221287-91-1-127
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error