1887

Abstract

Summary: An intracellular -asparaginase with antitumour activity was purified from a strain of Citrobacter. The optimum conditions for enzyme production by fermentation on scales up to 2700 l were investigated. Highest enzyme yield was obtained in corn-steep liquor medium (9·2%, w/v) at 37 °C. Oxygen limitation was not necessary for high enzyme yield. A total recovery of 4·3% from nucleic-acid-free extract and a 180-fold increase in specific activity were obtained after purification. The specific activity of the purified preparation was 45 i.u./mg protein. The enzyme hydrolysed -asparagine and -glutamine at 7 and 5%, respectively, of its activity toward -asparagine, but -glutaminase activity could be demonstrated only at substrate concentrations above 5 m. The values for -asparagine and -asparagine were 2·6 × 10 and 1·4 × 10 respectively. The anti-lymphoma activity of the enzyme was demonstrated with Gardner lymphosarcoma and was found only slightly less potent that Crasnitin, the most active asparaginase so far tested in this system.

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1975-11-01
2021-10-18
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