1887

Abstract

SUMMARY: A study has been made of the levels of 6-methylaminopurine and 5-methyl-cytosine in the DNA of during thymine deprivation. While DNA synthesis was inhibited by thymine deprivation, DNA methylation continued. Base analysis indicated that this aberrant methylation involved an increase solely in the amount of 5-methylcytosine. These aberrant 5-methylcytosine residues were removed from the DNA during continued growth of bacteria in medium lacking thymine. In contrast, 5-methylcytosine residues synthesized during normal growth were relatively unaffected by thymine deprivation. The results are interpreted to indicate that the extensive DNA damage which occurs during thymine deprivation is due in part to exonuclease digestion of regions of DNA containing aberrant 5-methylcytosine residues.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-90-2-347
1975-10-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/90/2/mic-90-2-347.html?itemId=/content/journal/micro/10.1099/00221287-90-2-347&mimeType=html&fmt=ahah

References

  1. Anagnostopoulos C., Spizizen J. 1961; Requirements for transformation in Bacillus subtilis. Journal of Bacteriology 81:741–746
    [Google Scholar]
  2. Buick R. N., Harris W. J. 1975; Thymineless death in Bacillus subtilis. Journal of General Microbiology 88:115–122
    [Google Scholar]
  3. Freifelder D. 1967; Lack of a relation between deoxyribonucleic acid methylation and thymineless death in E. coli. Journal of Bacteriology 93:1732–1733
    [Google Scholar]
  4. Fujimoto D., Srinivasan P. R., Broke E. 1965; On the nature of deoxyribonucleic acid methylases. Biological evidence for the multiple nature of the enzymes. Biochemistry 4:2849–2855
    [Google Scholar]
  5. Gold M., Hurwitz J. 1963; The enzymic methylation of nucleic acids. Cold Spring Harbor Symposia on Quantitative Biology 28:149–156
    [Google Scholar]
  6. Gold M., Hurwitz J. 1964; The enzymic methylation of ribonucleic acid and deoxyribonucleic acid. Journal of Biological Chemistry 239:3866–3874
    [Google Scholar]
  7. Harris W. J., Barr G. C. 1971; Structural features of DNA in competent Bacillus subtilis. Molecular and General Genetics 113:316–330
    [Google Scholar]
  8. Kirby K. S. 1957; A new method for the isolation of deoxyribonucleic acids: evidence on the nature of bonds between DNA and protein. Biochemical Journal 66:495–504
    [Google Scholar]
  9. Kirby K. S. 1958; Preparation of some DNA-protein complexes from rat liver homogenates. Biochemical Journal 70:260–265
    [Google Scholar]
  10. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  11. Magee P. N., Faber E. 1962; Toxic liver injury and carcinogenesis: methylation of rat liver nucleic acids by dimethylnitrosamine in vivo. Biochemical Journal 83:114–124
    [Google Scholar]
  12. Reiter H., Strauss B. 1965; Repair of damage induced by a monofunctional alkylating agent in a transformable, ultraviolet sensitive strain of Bacillus subtilis. Journal of Molecular Biology 14:179–194
    [Google Scholar]
  13. Reiter H., Strauss B., Robbins M., Maroone R. 1967; Nature of the repair of methylmethane-sulphonate induced damage in Bacillus subtilis. Journal of Bacteriology 93:1056–1062
    [Google Scholar]
  14. Rubery E. D., Newton A. A. 1971; A simple paper chromatographic method for separation of methylated adenines and cytosine from the major bases found in nucleic acids. Analytical Biochemistry 42:149–154
    [Google Scholar]
  15. Ryan A. M., Borek E. 1971; Methylation of DNA in ultra-violet irradiated bacteria. Biochimica et biophysica acta 240:203–214
    [Google Scholar]
  16. Strauss B. 1962; Differential destruction of the transforming activity of damaged deoxyribonucleic acid by a bacterial enzyme. Proceedings of the National Academy of Sciences of the United States of America 48:1670–1675
    [Google Scholar]
  17. Strauss B., Coyle M., Robbins M. 1968; Alkylation damage and its repair. Symposia on Quantitative Biology 33:277–287
    [Google Scholar]
  18. Theil E. C., Zamenhof S. 1963; Studies on 6-methylaminopurine in bacterial DNA. Journal of Biological Chemistry 238:3058–3064
    [Google Scholar]
  19. Tweats D. J., Pinney R. J., Smith J. T. 1974; R-factor mediated nuclease activity involved in thymineless elimination. Journal of Bacteriology 118:790–795
    [Google Scholar]
  20. Whittield B. L., Billen D. 1972; In vivo methylation of E. coli DNA following U.V. and X-irradiation. Journal of Molecular Biology 63:363–372
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-90-2-347
Loading
/content/journal/micro/10.1099/00221287-90-2-347
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error