1887

Abstract

SUMMARY: The isolation and properties of a mutant of K12 that is totally unable to take up and utilize gluconate are described. Genetical analysis shows this phenotype to be associated with two lesions. One phenotype, designated GntM, is the result of a mutation in a gene co-transducible with the other, designated GntS, is the result of a mutation in a gene () co-transducible with The GntS-phenotype differs little from that of wild-type cells, but GntM GntS organisms grow on gluconate only after a prolonged lag and form a gluconate uptake system that is strongly repressed by pyruvate. Moreover, such GntM mutants readily give rise to further mutants that form a gluconate uptake system, gluconate kinase and 6-phosphogluconate dehydratase constitutively; in partial diploids, this constitutivity is recessive to the inducible character. It is postulated that the GntM phenotype is due to malfunction of a negative control gene , and that specifies the activity of a gluconate uptake system.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-90-2-321
1975-10-01
2020-01-23
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-90-2-321
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error