1887

Abstract

SUMMARY: The particulate fraction of disrupted grown under cyanide-evolving conditions was unable to oxidize ascorbate plus -tetra-methyl--phenylenediamine (TMPD), but oxidized NADH and succinate by a linear respiratory pathway which was very resistant to inhibition by cyanide. When the bacteria were grown under conditions where little cyanide evolution occurred, particulate fractions developed the ability to oxidize ascorbate-TMPD by a pathway highly sensitive to cyanide inhibition; respiratory activity with NADH and succinate proceeded via both the cyanide-sensitive and -resistant pathways. Studies with respiratory inhibitors, and the cytochrome compositions of the fractions derived from cultures grown under both conditions, are presented. A soluble, carbon monoxide-binding cytochrome was found, and this appears similar to those found recently in , methylotrophic bacteria and the marine pseudomonad B16.

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1975-10-01
2024-04-23
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References

  1. Ackrell B. A. C., Jones C. W. 1971; The respiratory system of Azotobacter vinelandii. I. Properties of phosphorylating respiratory membranes. European Journal of Biochemistry 20:22–28
    [Google Scholar]
  2. Arima K., Oka T. 1965; Cyanide resistance in Achromobacter. I. Induced formation of cytochrome a2 and its role in cyanide-resistant respiration. Journal of Bacteriology 90:734–743
    [Google Scholar]
  3. Bartsch R. G. 1967; Spectroscopic properties of purified cytochromes of photosynthetic bacteria. In Bacterial Photosynthesis pp. 475–494 Gest H, Vernon L.P, San Pietro A. Edited by Antioch, Ohio: Antioch Press.;
    [Google Scholar]
  4. Bauchop T., Elsden S. R. 1960; The growth of micro-organisms in relation to their energy supply. Journal of General Microbiology 23:457–469
    [Google Scholar]
  5. Bendall D. S., Bonner W. D. 1971; Cyanide-insensitive respiration in plant mitochondria. Plant Physiology 47:236–245
    [Google Scholar]
  6. Chance B. 1961; Cytochrome o. In Haematin Enzymes pp. 433–435 Falk J.E, Lemberg R., Morton R.K. Edited by Oxford ,London, New York and Paris: Pergamon Press.;
    [Google Scholar]
  7. Daniel R. M. 1970; The electron transport system of Acetobacter suboxydans with particular reference to cytochrome o. Biochimica et biophysica acta 216:328–341
    [Google Scholar]
  8. Downie J. A., Garland P. B. 1973; An antimycin A- and cyanide-resistant variant of Candida utilis arising during copper-limited growth. Biochemical Journal 134:1051–1061
    [Google Scholar]
  9. Epstein J. 1947; Estimation of microquantities of cyanide. Analytical Chemistry 19:272–274
    [Google Scholar]
  10. Gel’man N. S., Lukoyanova M. A., Ostrovskii D. N. 1967 Respiration and Phosphorylation of Bacteria pp. 140–142 New York: Plenum Press.;
    [Google Scholar]
  11. Gornall A. G., Bardawill C. S., David M. M. 1949; Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry 177:751–763
    [Google Scholar]
  12. Von Jagow G., Klingenberg M. 1970; Pathways of hydrogen in mitochondria of Saccharomyces carlsbergensis. European Journal of Biochemistry 12:583–592
    [Google Scholar]
  13. Jones C. W., Redfearn E. R. 1966; Electron transport in Azobacter vinelandii. Biochimica et biophysica acta 113:467–481
    [Google Scholar]
  14. Jones C. W., Redfearn E. R. 1967; The cytochrome system of Azobacter vinelandii. Biochimica et biophysica acta 143:340–353
    [Google Scholar]
  15. Knowles C. J., Calcott P. H., MacLeod R. A. 1974; Periplasmic CO-binding c-type cytochrome in a marine bacterium. FEBS Letters 49:78–83
    [Google Scholar]
  16. Lambowitz A. M., Slayman C. W. 1971; Cyanide-resistant respiration in Neurospora crassa. Journal of Bacteriology 108:1087–1096
    [Google Scholar]
  17. McFeters G. A., Wilson D. F., Strobel G. A. 1970; Cytochromes in a cyanide-resistant strain of Bacillus cereus. Canadian Journal of Microbiology 16:1221–1226
    [Google Scholar]
  18. Michaels R., Corpe W. A. 1965; Cyanide formation by Chromobacterium violaceum. Journal of Bacteriology 89:106–112
    [Google Scholar]
  19. Miller J. H. 1972 Experiments in Molecular Genetics p. 431 Cold Spring Harbor: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  20. Pudek M. R., Bragg P. D. 1974; Inhibition of the respiratory chain oxidases of Escherichia coli. Archives of Biochemistry and Biophysics 164:682–693
    [Google Scholar]
  21. Ray S. K., Cross G. A. M. 1972; Branched electron transport chain in Trypanosoma mega. Nature New Biology 237:174–I75
    [Google Scholar]
  22. Schonbaum G. R., Bonner W. D., Storey B. T., Bahr J. T. 1971; Specific inhibition of the cyanide- insensitive respiratory pathway in plant mitochondria by hydroxamic acids. Plant Physiology 47:124–128
    [Google Scholar]
  23. Slater E. C. 1967; Application of inhibitors and uncouplers for a study of oxidative phosphorylation. Methods in Enzymology 10:48–57
    [Google Scholar]
  24. Sneath P. H. A. 1966; Identification methods applied to Chromobacterium. In Identification Methods for Microbiologists part A pp. 15–20 Gibbs B.M, Skinner F.A. Edited by London and New York: Academic Press.;
    [Google Scholar]
  25. Tonge G. M., Knowles C. J., Harrison D. E. F., Higgins I. J. 1974; Metabolism of one carbon compounds: cytochromes of methane- and methanol-utilising bacteria. FEBS Letters 44:106–110
    [Google Scholar]
  26. Weston J. A., Collins P. A., Knowles C. J. 1974; The respiratory system of the marine bacterium Beneckea natriegens. II. Terminal branching of respiration to oxygen and resistance to inhibition by cyanide. Biochimica et biophysica acta 368:148–157
    [Google Scholar]
  27. Weston J. A., Knowles C. J. 1973; A soluble CO-binding c-type cytochrome from the marine bacterium Beneckea natriegens. Biochimica et biophysica acta 305:11–18
    [Google Scholar]
  28. Weston J. A., Knowles C. J. 1974; The respiratory system of the marine bacterium Beneckea natriegens. I. Cytochrome composition. Biochimica et biophysica acta 333:228–236
    [Google Scholar]
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