1887

Abstract

SUMMARY: Chitin synthase of was present in the ‘microsomal’ fraction (100000 precipitate), the ‘cell-wall’ fraction (2000 precipitate) and the ‘mitochondrial’ fraction (10000 g precipitate). The properties of the ‘microsomal’ enzyme were investigated. The pH optimum was between 5·8 and 6·2, and the temperature optimum was between 31 and 33 °C. The for UDP -acetyl--glucosamine was 1·8 m. The enzyme was stimulated by Mg and a slight stimulation was also effected by -acetyl--glucosamine. Soluble chitodextrins were inhibitory. A pH-dependent, heat-stable inhibitor of chitin synthase activity was present in the soluble cytoplasm from the mycelium. The effects of aeration and glucose concentration on enzyme production in growing cultures were also investigated; maximum specific activity of chitin synthase was associated with the cessation of exponential growth.

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1975-10-01
2021-05-15
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