SUMMARY: Chitin synthase of was present in the ‘microsomal’ fraction (100 000 precipitate), the ‘cell-wall’ fraction (2000 precipitate) and the ‘mitochondrial’ fraction (10000 g precipitate). The properties of the ‘microsomal’ enzyme were investigated. The pH optimum was between 5·8 and 6·2, and the temperature optimum was between 31 and 33°C. The for UDP -acetyl-D-glucosamine was 1·8 mM. The enzyme was stimulated by Mg and a slight stimulation was also effected by -acetyl-D-glucosamine. Soluble chitodextrins were inhibitory. A pH-dependent, heat-stable inhibitor of chitin synthase activity was present in the soluble cytoplasm from the mycelium. The effects of aeration and glucose concentration on enzyme production in growing cultures were also investigated; maximum specific activity of chitin synthase was associated with the cessation of exponential growth.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error