1887

Abstract

Summary: The concentrations of free amino acids, particularly alanine, altered as stromata developed on germinating sclerotia. Lipid reserves in the sclerotium provided the principal carbon and energy source, and the nutrients required for stromatal growth appeared to be provided by the sclerotial tissues immediately beneath the point of attachment of the stroma. Alkaloid did not disappear from the sclerotia during germination. Very high levels of polyol, particularly mannitol, were attained in the stromata as germination progressed; the synthesis of polyol was usually accompanied by that of soluble sugars, notably glucose. In an attempt to ascertain the role of mannitol in germination, the relationship between polyol and soluble sugars was investigated in excised stromata after their submergence in buffered media containing a variety of soluble carbohydrates. Mannitol was synthesized under these conditions by way of phosphorylated intermediates, and chiefly in the capitulum, after rapid, and probably passive, absorption of the exogenously supplied sugar. The tissues equilibrated rapidly with the supplied carbohydrate, whose concentration was maintained by further uptake whilst mannitol was being synthesized. The concentrations of assimilated glucose and synthesized mannitol tended toward the same value during incubation and the results suggested that the synthesis was compartmentalized within the stromata. Very little exogenously supplied mannitol was assimilated.

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1975-09-01
2021-10-15
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References

  1. Caldwell P. C. 1960; The phosphorus metabolism of squid axons and its relationship to the active transport of sodium. Journal of Physiology 152:545–560
    [Google Scholar]
  2. Caldwell P. C., Hodgkin A. L., Keynes R. D., Shaw T. I. 1960; The effect of injecting energy-rich phosphate compounds on the active transport of ions in the giant axons of Loligo. Journal of Physiology 152:561–590
    [Google Scholar]
  3. Cooke R. C., Mitchell D. T. 1966; Sclerotium size and germination in Claviceps purpurea. Transactions of the British Mycological Society 49:95–100
    [Google Scholar]
  4. Cooke R. C., Mitchell D. T. 1967; Germination pattern and capacity for repeated stroma formation in Claviceps purpurea. Transactions of the British Mycological Society 50:275–283
    [Google Scholar]
  5. Cooke R. C., Mitchell D. T. 1969; Sugars and polyols in sclerotia of Claviceps purpurea, C. nigricans and Sclerotinia curreyana during germination. Transactions of the British Mycological Society 52:365–372
    [Google Scholar]
  6. Cooke R. C., Mitchell D. T. 1970; Carbohydrate physiology of sclerotia of Claviceps purpurea during dormancy and germination. Transactions of the British Mycological Society 54:93–99
    [Google Scholar]
  7. Corbett K., Dickerson A. G., Mantle P. G. 1974; Metabolic studies on Claviceps purpurea during parasitic development on rye. Journal of General Microbiology 84:39–58
    [Google Scholar]
  8. Dickerson A. G. 1972; A β-d-fructofuranosidase from Claviceps purpurea. Biochemical Journal 129:263–272
    [Google Scholar]
  9. Dickerson A. G., Mantle P. G., Szczyrbak C. A. 1970; Autolysis of extracellular glucans produced in vitro by a strain of Claviceps fusiformis. Journal of General Microbiology 60:403–415
    [Google Scholar]
  10. Garay A. S. 1958; Der Zusammenhang zwischen Fruchtkörperbildung und Atmung im Falle des Mutter-korns unter besonderer Berücksichtigung der Bedeutung der Fumärsaure. Physiologia plantarum 11:48–55
    [Google Scholar]
  11. Glynn I. M. 1956; Sodium and potassium movements in human red cells. Journal of Physiology 134:278–310
    [Google Scholar]
  12. Mantle P. G., Morris L. J., Hall S. W. 1969; Fatty acid composition of sphaecelial and sclerotial growth forms of Claviceps purpurea in relation to the production of ergoline alkaloids in culture. Transactions of the British Mycological Society 53:441–447
    [Google Scholar]
  13. Mitchell D. T., Cooke R. C. 1968a; Some effects of temperature on germination and longevity of sclerotia in Claviceps purpurea. Transactions of the British Mycological Society 51:721–729
    [Google Scholar]
  14. Mitchell D. T., Cooke R. C. 1968b; Water uptake, respiration pattern and lipid utilisation in sclerotia of Claviceps purpurea during germination. Transactions of the British Mycological Society 51:731–736
    [Google Scholar]
  15. Morris L. T., Hall S. W. 1966; The structure of the glycerides of ergot oils. Lipids 1:188–196
    [Google Scholar]
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