Correlation of Virulence with Secretion of Three Wall-degrading Enzymes in Isolates of Obtained after Mutagen Treatment Free

Abstract

Summary: Correlation and regression analyses were carried out between the virulence (expressed as growth rate in apple fruits) and the secretion of three host wall-degrading enzymes by 119 isolates of , most of which had been obtained following exposure of conidia to -methyl--nitro--nitroso-guanidine. Virulence was found to be significantly correlated ( < 0·01) with α--arabinofuranosidase, but not with pectin esterase or, where enzyme inter-dependence had been statistically eliminated, with polygalacturonase. Approximtely 35% of the total variability in virulence could be accounted for in terms of the three enzymes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-90-1-32
1975-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/90/1/mic-90-1-32.html?itemId=/content/journal/micro/10.1099/00221287-90-1-32&mimeType=html&fmt=ahah

References

  1. Albersheim P., Anderson A. 1971; Host-pathogen interactions. III. Proteins from plant cell walls inhibit polygalacturonases secreted by plant pathogens. Proceedings of the National Academy of Sciences of the United States of America 68:1815–1819
    [Google Scholar]
  2. Albersheim P., Jones T. M., English P. D. 1969; The cell wall in relation to infective processes. Annual Review of Phytopathology 7:171–194
    [Google Scholar]
  3. Allen A. K., Neuberger A. 1973; The purification and properties of the lectin from potato tubers, a hydroxyproline-containing glycoprotein. Biochemical Journal 135:307–314
    [Google Scholar]
  4. Archer S. A. 1973 Physiological aspects of brown rot of apple. Ph. D. thesis University of Bristol;
    [Google Scholar]
  5. Barker K. R., Walker J. C. 1962; Relationship of pectolytic and cellulytic enzyme production by strains of Pellicularia filamentosa to their pathogenicity. Phytopathology 52:1119–1128
    [Google Scholar]
  6. Bateman D. F., Millar R. L. 1966; Pectic enzymes in tissue degradation. Annual Review of Phyto-pathology 4:119–146
    [Google Scholar]
  7. Byrde R. J. W., Fielding A. H. 1968; Pectin methyl-trans-eliminase as the maceration factor of Sclerotinia fructigena and its significance in brown rot of apple. Journal of General Microbiology 52:287–297
    [Google Scholar]
  8. Byrde R. J.W., Fielding A. H., Archer S. A., Davies E. 1973; The role of extracellular enzymes in the rotting of fruit tissue by Sclerotinia fructigena. In Fungal Pathogenicity and the Plant’s Response pp. 39–52 Byrde R. J. W., Cutting V. Edited by London: Academic Press;
    [Google Scholar]
  9. Cooper R. M., Wood R. K. S. 1973; Induction of synthesis of extracellular cell-wall degrading enzymes in vascular wilt fungi. Nature; London: 246309–311
    [Google Scholar]
  10. Dingle J., Reid W. W., Solomons G. L. 1953; The enzymic degradation of pectin and other polysaccharides. II. Application of the ‘cup-plate’ assay to the estimation of enzymes. Journal of Science of Food and Agriculture 4:149–155
    [Google Scholar]
  11. Eriksson K.-E., Goodell E. W. 1974; Pleiotropic mutants of the wood-rotting fungus Polyporus adustus lacking cellulase, mannanase and xylanase. Canadian Journal of Microbiology 20:371–378
    [Google Scholar]
  12. Federation of British Plant Pathologists 1973 A Guide to the Use of Terms in Plant Pathology. Phytopathological Papers17 Kew, Surrey: Commonwealth Mycological Institute;
    [Google Scholar]
  13. Fuchs A., Jobson J. A., Wonts W. N. 1965; Arabanases in phytopathogenic fungi. Nature; London: 206714–715
    [Google Scholar]
  14. Hall R. 1972; Pathogenicity of Monilinia fructicola. III. Factors influencing lesion expansion. Phytopathologische Zeitschrift 73:27–38
    [Google Scholar]
  15. Hislop E. C., Shellis C., Fielding A. H., Bourne F. J., Chidlow J. W. 1974; Antisera produced to purified extracellular pectolytic enzymes from Sclerotinia fructigena. Journal of General Microbiology 83:135–143
    [Google Scholar]
  16. Hoffmann G. M. 1970; Kernverhaltnisse bei Monilia fructigena und M. laxa. Phytopathologische Zeitschrift 68:143–154
    [Google Scholar]
  17. Hoffmann G. M. 1972; Heterokaryose bei Wildstämmen von Moniliniafructigena. Phytopathologische Zeitschrift 73:326–340
    [Google Scholar]
  18. Howell H. E. 1972 Production of extracellular enzymes by Sclerotinia fructigena Aderh. & Ruhl. Some observations on their importance in pathogenicity and on their inheritance. Ph. D. thesis University of Bristol;
    [Google Scholar]
  19. Knee M. 1975; Soluble and wall-bound glycoproteins of apple fruit tissue. Phytochemistry in the Press
    [Google Scholar]
  20. Laborda F., Fielding A. H., Byrde R. J.W. 1973; Extra- and intra-cellular α-l-arabinofuranosidase of Sclerotinia fructigena. Journal of General Microbiology 79:321–329
    [Google Scholar]
  21. Morrall R. A.A., Duczek L. J., Sheard J. W. 1972; Variations and correlations with and between morphology, pathogenicity and pectolytic enzyme activity in Sclerotinia from Saskatchewan. Canadian Journal of Botany 50:767–786
    [Google Scholar]
  22. Smith W. K. 1958; A survey of the production of pectic enzymes by plant pathogenic and other bacteria. Journal of General Microbiology 18:33–41
    [Google Scholar]
  23. Tatareau J.-C., Auriol P. 1972; Correlation entre la virulence de Colletotrichum lagenarium et son aptitude à degrader les polyosides des parois cellulaires d’un hôte sensible. Comptes rendus hebdomadaire des séances de l’Academie des sciences 274:1304–1306
    [Google Scholar]
  24. Touzé A. 1973; Anthracnose fungus virulence and changes induced in host cell wall. Abstracts of Papers, 2nd International Congress of Plant Pathology held at Minneapolis, U. S.A. 0115
    [Google Scholar]
  25. Volkova V. P. 1971; Polygalacturonase activity of Pseudomonas tabaci. Mikrobiologicheskii zhurnal 33:112–113
    [Google Scholar]
  26. Wood R. K.S. 1967 Physiological Plant Pathology. Oxford and Edinburgh: Blackwell Scientific Publications;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-90-1-32
Loading
/content/journal/micro/10.1099/00221287-90-1-32
Loading

Data & Media loading...

Most cited Most Cited RSS feed