1887

Abstract

SUMMARY: The percentage of bacterial carbon that was derived from exogenous labelled compounds present in the medium during the growth of some methylotrophs on trimethylamine or on non-C compounds was determined. Less than 10% of bacterial carbon was derived from acetate during the growth of the obligate methylotrophs 46 and 1, and of the restricted facultative methylotroph 6; the other restricted facultative methylotroph W3A1 gave a value of 18%. Corresponding values for three typical facultative methylotrophs growing on trimethylamine were 41. 42 and 52%. Aspartate, fructose, pyruvate and succinate made only a small percentage contribution (0.4 to 12%) to bacterial carbon in 46, 1, W3AI and 6. Washed suspensions of 46, 1, 1 and 6, all grown on trimethylamine, assimilated labelled acetate only in the presence of trimethylamine and there was a linear relationship between the amount of acetate assimilated and the amount of trimethylamine oxidized. Organisms 46, 1, 1 and 6 assimilated C from labelled acetate predominantly into lipid (except 6), glutamate, arginine, proline and leucine, whereas the typical facultative methylotrophs assimilated C from acetate into lipid, nucleic acid and all the protein amino acids. These results are consistent with the presence of a deficient tricarboxylic acid cycle in the obligate methylotrophs and in the restricted facultative methylotrophs.

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/content/journal/micro/10.1099/00221287-90-1-169
1975-09-01
2021-10-19
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